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Initially, 162<jats:italic>Staphylococcus<\/jats:italic>spp. isolates were subjected to<jats:italic>in vitro<\/jats:italic>antimicrobial sensitivity tests. Of these, 35 presented antimicrobial multi-drug resistance phenotypes. These 35 isolates were then referred for the detection of<jats:italic>norA, norB, norC, msrA, mgrA, tet-38,<\/jats:italic>and<jats:italic>lmrS<\/jats:italic>genes, all of which feature in multi-drug efflux systems. In the isolates carrying the genes, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of PPy-NPs and<jats:italic>Moringa oleifera<\/jats:italic>aqueous extract were determined. In the molecular analysis of the 35 isolates<jats:italic>norA, norC, tet-38,<\/jats:italic>and<jats:italic>msrA<\/jats:italic>genes were detected and for the other genes<jats:italic>norB, lmrS<\/jats:italic>and<jats:italic>mgrA<\/jats:italic>there was no amplification. Antimicrobial activity was verified of PPy-NPs and aqueous extract of<jats:italic>Moringa oleifera<\/jats:italic>in<jats:italic>Staphylococcus<\/jats:italic>spp. carrying multi-drug efflux system genes. We concluded that there are multi-drug efflux system genes present in the<jats:italic>Staphylococcus<\/jats:italic>spp. from the agricultural environment in Northeast Brazil, and that aqueous extract of<jats:italic>Moringa oleifera<\/jats:italic>and PPy-NPs show bactericidal activity against these isolates.<\/jats:p>","DOI":"10.1017\/s0022029920000874","type":"journal-article","created":{"date-parts":[[2020,9,22]],"date-time":"2020-09-22T07:37:11Z","timestamp":1600760231000},"page":"309-314","source":"Crossref","is-referenced-by-count":8,"title":["Antimicrobial activity of polypyrrole nanoparticles and aqueous extract of<i>Moringa oleifera<\/i>against<i>Staphylococcus<\/i>spp. carriers of multi-drug efflux system genes isolated from dairy farms"],"prefix":"10.1017","volume":"87","author":[{"given":"Jos\u00e9 Givanildo da","family":"Silva","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana de","family":"Barros","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nataly Diniz de Lima","family":"Santos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patr\u00edcia Maria Guedes","family":"Paiva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thiago Henrique","family":"Napole\u00e3o","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Jos\u00e9 de","family":"Sena","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mateus Matiuzzi da","family":"Costa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helinando Pequeno de","family":"Oliveira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Aparecida Scatamburlo","family":"Moreira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rinaldo Aparecido","family":"Mota","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2020,9,22]]},"reference":[{"key":"S0022029920000874_ref51","first-page":"1857","article-title":"Antibacterial activity of bark extracts of Moringa oleifera Lam. against some selected bacteria","volume":"27","author":"Zaffer","year":"2014","journal-title":"Pakistan Journal of Pharmaceutical Science"},{"key":"S0022029920000874_ref50","doi-asserted-by":"publisher","DOI":"10.1016\/j.snb.2014.07.018"},{"key":"S0022029920000874_ref47","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.50.3.1104-1107.2006"},{"key":"S0022029920000874_ref44","doi-asserted-by":"publisher","DOI":"10.1017\/S0022029917000851"},{"key":"S0022029920000874_ref43","doi-asserted-by":"crossref","first-page":"1251","DOI":"10.1590\/1678-4162-10681","article-title":"Atividade antimicrobiana e citotoxicidade in vitro do \u00f3leo essencial de Tagetes minuta L. visando \u00e0 aplica\u00e7\u00e3o no controle da mastite bovina","volume":"71","author":"Sperandio","year":"2019","journal-title":"Arquivo Brasileiro de Medicina Veterin\u00e1ria e Zootecnia"},{"key":"S0022029920000874_ref42","doi-asserted-by":"publisher","DOI":"10.9790\/5736-07211115"},{"key":"S0022029920000874_ref39","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2013.09.028"},{"key":"S0022029920000874_ref36","doi-asserted-by":"publisher","DOI":"10.1080\/14786419.2017.1389932"},{"key":"S0022029920000874_ref33","doi-asserted-by":"publisher","DOI":"10.1007\/s12551-018-0404-9"},{"key":"S0022029920000874_ref31","doi-asserted-by":"publisher","DOI":"10.1590\/1983-21252016v29n328rc"},{"key":"S0022029920000874_ref28","doi-asserted-by":"publisher","DOI":"10.1111\/jam.12882"},{"key":"S0022029920000874_ref26","unstructured":"Micas, AFD (2008) Avalia\u00e7\u00e3o da atividade bactericida e bacteri\u00f3stica da violace\u00edna. 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