{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,18]],"date-time":"2025-09-18T10:13:20Z","timestamp":1758190400269,"version":"3.44.0"},"reference-count":20,"publisher":"Cambridge University Press (CUP)","issue":"1","license":[{"start":{"date-parts":[[2025,7,25]],"date-time":"2025-07-25T00:00:00Z","timestamp":1753401600000},"content-version":"unspecified","delay-in-days":174,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Dairy Research"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Buffalo are shy breeders and poor fertility traits are a major hindrance in exploiting the production potential of the animal. This study hypothesizes that polymorphisms in the luteinizing hormone beta (LH\u03b2) gene can affect oestrus behaviour in buffaloes. A total of 100 animals were screened by calculating the heat index (threshold-50) and animals were categorized into two groups (Group1 &gt; 50, Group2 &lt; 50). Animals were subjected to blood sampling, genomic DNA isolation, specific primer based polymerization and sequencing of amplicons. A total of six genomic variations were identified in the gene. c.V15M was a non synonymous mutation found in line with the Hardy Weinberg Equilibrium and was significantly associated with the trait. Functional impact of the variation was determined by three-dimensional structure of the protein. Effect of c.V15M on the functionality of the gene was evident and hypothesis was supported so this can potentially be used as a marker for the future development of superior animal breed or regulating the expression of the gene to get the optimal oestrus cyclicity in river buffalo of Pakistan.<\/jats:p>","DOI":"10.1017\/s0022029925000342","type":"journal-article","created":{"date-parts":[[2025,7,25]],"date-time":"2025-07-25T09:31:36Z","timestamp":1753435896000},"page":"61-65","source":"Crossref","is-referenced-by-count":0,"title":["Functional impact of c.V15M variation in LH\u03b2 gene on silent oestrus behaviour in river buffalo of Pakistan"],"prefix":"10.1017","volume":"92","author":[{"given":"Asif","family":"Nadeem","sequence":"first","affiliation":[{"name":"Virtual University of Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haroon","family":"Iqbal","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Somia","family":"Shehzadi","sequence":"additional","affiliation":[{"name":"University of Lahore Institute of Medical Laboratory Technology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maryam","family":"Javed","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2025,7,25]]},"reference":[{"key":"S0022029925000342_ref10","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2007-0669"},{"key":"S0022029925000342_ref1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygcen.2007.03.012"},{"key":"S0022029925000342_ref4","doi-asserted-by":"publisher","DOI":"10.1080\/10495398.2016.1244068"},{"key":"S0022029925000342_ref11","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1973.tb03121.x"},{"key":"S0022029925000342_ref12","first-page":"46","article-title":"Seasonality in buffaloes reproduction","author":"Phogat","year":"2016","journal-title":"International Journal of Plant, Animal and Environmental Sciences"},{"key":"S0022029925000342_ref16","first-page":"2","article-title":"Multiple sequence alignment using ClustalW and ClustalX","author":"Thompson","year":"2002","journal-title":"Current Protocols in Bioinformatics\/editoral Board"},{"key":"S0022029925000342_ref20","first-page":"816","article-title":"Comparative effects of diverse vertebrate gonadotropins on androgen formation in vitro from testes of rooster and mice","author":"Yu","year":"1987","journal-title":"Biology of Reproduction"},{"key":"S0022029925000342_ref13","doi-asserted-by":"publisher","DOI":"10.5713\/ajas.17.0679"},{"key":"S0022029925000342_ref8","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2013.09.018"},{"key":"S0022029925000342_ref2","doi-asserted-by":"publisher","DOI":"10.1017\/S1751731109991169"},{"key":"S0022029925000342_ref7","first-page":"149","article-title":"Facets of oestrus behaviour in Surti buffaloes","author":"Gamit","year":"2015","journal-title":"Advances in Dairy Research"},{"key":"S0022029925000342_ref18","doi-asserted-by":"publisher","DOI":"10.1016\/0044-8486(95)01009-2"},{"key":"S0022029925000342_ref5","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygcen.2004.12.017"},{"key":"S0022029925000342_ref3","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2006-852"},{"key":"S0022029925000342_ref19","doi-asserted-by":"publisher","DOI":"10.1006\/gcen.1996.0168"},{"key":"S0022029925000342_ref14","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2004.07.009"},{"key":"S0022029925000342_ref9","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.0500457"},{"key":"S0022029925000342_ref6","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2010.06.002"},{"volume-title":"Molecular Cloning. A Laboratory Manual. 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