{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T16:46:01Z","timestamp":1772642761705,"version":"3.50.1"},"reference-count":10,"publisher":"Cambridge University Press (CUP)","issue":"3","license":[{"start":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T00:00:00Z","timestamp":1761523200000},"content-version":"unspecified","delay-in-days":87,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Dairy Research"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    This study was undertaken to investigate the molecular detection of biofilm-producing\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    (\n                    <jats:italic>S. aureus<\/jats:italic>\n                    ) from goat mastitis. An overall 384 milk samples were initially screened for subclinical mastitis (SCM) followed by molecular characterization of\n                    <jats:italic>S. aureus<\/jats:italic>\n                    isolates. The biofilm formation was assessed using Congo Red agar (CRA), a microtiter plate and the presence of the\n                    <jats:italic>icaA<\/jats:italic>\n                    gene. The results revealed a molecular prevalence of 53.24% (115\/216) for pathogenic\n                    <jats:italic>S. aureus<\/jats:italic>\n                    in milk samples of goats. The phenotypic prevalence of biofilm production by CRA and microtitre methods was recorded to be 38.26% (44\/115) and 26.96% (31\/115) respectively, while the molecularly confirmed biofilm-forming\n                    <jats:italic>S. aureus<\/jats:italic>\n                    through polymerase chain reaction targeting\n                    <jats:italic>icaA<\/jats:italic>\n                    gene was 58.26% (67\/115). The phylogenetic analysis of\n                    <jats:italic>icaA<\/jats:italic>\n                    gene revealed high identity between sequences of study isolates and the isolates of other neighbouring countries. The antibiogram profiling of pathogenic\n                    <jats:italic>S. aureus<\/jats:italic>\n                    showed increased resistance to cefoxitin and oxytetracycline followed by gentamicin. Out of 115, 45.22% (52\/115) were declared as multiple drug resistant with multiple antibiotic resistance index greater than 0.2. The study concluded that biofilm-producing\n                    <jats:italic>S. aureus<\/jats:italic>\n                    strains are considered to be a common cause of SCM in dairy goats of Pakistan and biofilm formation is associated with multidrug resistance of study isolates.\n                  <\/jats:p>","DOI":"10.1017\/s0022029925101192","type":"journal-article","created":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T07:31:16Z","timestamp":1761550276000},"page":"325-328","source":"Crossref","is-referenced-by-count":0,"title":["Insights into epidemiology, molecular characterization and antibiogram profiling of\u00a0biofilm-forming\n                    <i>Staphylococcus aureus<\/i>\n                    isolated from goat mastitis"],"prefix":"10.1017","volume":"92","author":[{"given":"Muhammad Umar","family":"Javed","sequence":"first","affiliation":[{"name":"University of Veterinary and Animal Sciences"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muhammad","family":"Ijaz","sequence":"additional","affiliation":[{"name":"University of Veterinary and Animal Sciences"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arslan","family":"Ahmed","sequence":"additional","affiliation":[{"name":"University of Veterinary and Animal Sciences"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hamza","family":"Rasheed","sequence":"additional","affiliation":[{"name":"University of Veterinary and Animal Sciences"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muhammad Jawad","family":"Sabir","sequence":"additional","affiliation":[{"name":"University of Veterinary and Animal Sciences"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ali Abdullah","family":"Jabir","sequence":"additional","affiliation":[{"name":"University of Veterinary and Animal Sciences"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2025,10,27]]},"reference":[{"key":"S0022029925101192_ref7","doi-asserted-by":"publisher","DOI":"10.1007\/s11250-020-02331-5"},{"key":"S0022029925101192_ref1","doi-asserted-by":"publisher","DOI":"10.29261\/pakvetj\/2022.078"},{"key":"S0022029925101192_ref2","first-page":"193","article-title":"Biofilm forming multidrug resistant Staphylococcus aureus of dairy origin: molecular and evolutionary perspectives","volume":"24","author":"Ahmed","year":"2023","journal-title":"Iranian Journal of Veterinary Research"},{"key":"S0022029925101192_ref6","volume-title":"CLSI M100-ED29: 2019 Performance Standards for Antimicrobial Susceptibility Testing","year":"2019"},{"key":"S0022029925101192_ref8","first-page":"211","article-title":"Genotypic insights to Panton-Valentine leukocidin positive methicillin-resistant Staphylococcus aureus isolated from cattle mastitis","volume":"91","author":"Ijaz","year":"2024","journal-title":"Journal of Dairy Research 2022\u20132024"},{"key":"S0022029925101192_ref3","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2019.103755"},{"key":"S0022029925101192_ref11","doi-asserted-by":"publisher","DOI":"10.17582\/journal.pjz\/20191024181008"},{"key":"S0022029925101192_ref10","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2023.106456"},{"key":"S0022029925101192_ref9","first-page":"423","article-title":"Molecular dynamics and antimicrobial resistance pattern of \u03b2-lactam resistant coagulase positive Staphylococcus aureus isolated from goat mastitis","volume":"44","author":"Javed","year":"2024","journal-title":"Pakistan Veterinary Journal"},{"key":"S0022029925101192_ref4","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics10060633"}],"container-title":["Journal of Dairy Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0022029925101192","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T12:20:57Z","timestamp":1772626857000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0022029925101192\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8]]},"references-count":10,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2025,8]]}},"alternative-id":["S0022029925101192"],"URL":"https:\/\/doi.org\/10.1017\/s0022029925101192","relation":{},"ISSN":["0022-0299","1469-7629"],"issn-type":[{"value":"0022-0299","type":"print"},{"value":"1469-7629","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,8]]}}}