{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T11:01:51Z","timestamp":1791198111987,"version":"4.1.0"},"reference-count":79,"publisher":"Cambridge University Press (CUP)","license":[{"start":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T00:00:00Z","timestamp":1791158400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Dairy Research"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    This study focuses on the development of nanocapsules loaded with\n                    <jats:italic>Tagetes minuta<\/jats:italic>\n                    essential oil (TEO-NC) for milking management in sustainable dairy systems. The nanocapsules were prepared by nanoprecipitation, and their physicochemical properties were evaluated for 60\u00a0days at 4\u00b0C and 25\u00b0C. Antimicrobial and disinfectant activities were evaluated in the presence and absence of organic matter and on contaminated surfaces, such as rubber, stainless steel and ceramics. TEO-NCs had an average particle size of 266.67\u00a0\u00b1\u00a018.28\u00a0nm, polydispersity index (PdI)\u00a0&lt;0.12, zeta potential of 42.70\u00a0\u00b1\u00a01.08 and pH of 4.09\u00a0\u00b1\u00a00.08. Moreover, TEO-NCs showed no visual changes, and the physicochemical changes were minimal at the two temperatures tested. The minimum inhibitory concentration of TEO-NCs against\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    was 50% (v\/v). The disinfectant activity of TEO-NCs (100 and 50%, v\/v) was demonstrated against\n                    <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    in the absence of organic matter after 5, 15 and 30\u00a0min. Disinfectant activity against\n                    <jats:italic>S. aureus<\/jats:italic>\n                    was demonstrated after 15 and 30\u00a0min (100%, v\/v). In the presence of organic matter, the disinfectant activity of TEO-NCs (100%, v\/v) was effective only against\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    . Similarly, disinfectant activity of TEO-NCs against\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    was demonstrated after 15 and 30\u00a0min on the three surfaces tested. Our results highlight the potential of\n                    <jats:italic>T. minuta<\/jats:italic>\n                    essential oils, a natural product, which, when combined with nanocapsules, results in a potential alternative for teat disinfection in milking management, especially in the control of environmental mastitis.\n                  <\/jats:p>","DOI":"10.1017\/s002202992610257x","type":"journal-article","created":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T10:33:40Z","timestamp":1791196420000},"page":"1-9","source":"Crossref","is-referenced-by-count":0,"title":["Preparation, physicochemical characterisation, storage stability and\n                    <i>in vitro<\/i>\n                    disinfectant activity of\n                    <i>Tagetes minuta<\/i>\n                    (L.) essential oil-loaded polycaprolactone nanocapsules against mastitis pathogens"],"prefix":"10.1017","author":[{"given":"Daniela Aparecida","family":"Moreira","sequence":"first","affiliation":[{"name":"Universidade Federal de Santa Catarina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karin Possebon","family":"Bolzan","sequence":"additional","affiliation":[{"name":"Universidade Federal de Santa Catarina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leticia","family":"Mazzarino","sequence":"additional","affiliation":[{"name":"Universidade Federal de Santa Catarina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Beatriz","family":"Veleirinho","sequence":"additional","affiliation":[{"name":"Universidade Federal de Santa Catarina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luciana Aparecida","family":"Honorato","sequence":"additional","affiliation":[{"name":"Universidade Federal de Santa Catarina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luiz Filipe Dame","family":"Schuch","sequence":"additional","affiliation":[{"name":"Universidade Federal de Pelotas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luiz Carlos","family":"Pinheiro Machado Filho","sequence":"additional","affiliation":[{"name":"Universidade Federal de Santa Catarina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1882-5241","authenticated-orcid":false,"given":"Shirley","family":"Kuhnen","sequence":"additional","affiliation":[{"name":"Universidade Federal de Santa Catarina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2026,10,5]]},"reference":[{"key":"S002202992610257X_ref47","first-page":"561","article-title":"Nanoparticles - a review","volume":"5","author":"Mohanraj","year":"2006","journal-title":"Tropical Journal of Pharmaceutical Research"},{"key":"S002202992610257X_ref15","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2007.10.013"},{"key":"S002202992610257X_ref1","first-page":"441","article-title":"Acaricidal and insecticidal effects of essential oils against ectoparasites of veterinary importance","volume":"17","author":"Abbas","year":"2018","journal-title":"Boletin Latinoamericano y del Caribe de Plantas Medicinales y Arom\u00e1ticas"},{"key":"S002202992610257X_ref14","volume-title":"Methods for Dilution of Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically","year":"2015"},{"key":"S002202992610257X_ref18","doi-asserted-by":"publisher","DOI":"10.14295\/cs.v10i1.2623"},{"key":"S002202992610257X_ref24","first-page":"48","article-title":"The efficacy of Acacia nilotica extract as a disinfectant on addition silicone impressions: an in-vitro study","volume":"8","author":"Eltahir","year":"2020","journal-title":"International Journal of Herbal Medicine"},{"key":"S002202992610257X_ref42","doi-asserted-by":"publisher","DOI":"10.1080\/22311866.2019.1576542"},{"key":"S002202992610257X_ref52","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics11010108"},{"key":"S002202992610257X_ref62","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2007.01.006"},{"key":"S002202992610257X_ref76","doi-asserted-by":"publisher","DOI":"10.1016\/j.beem.2017.10.004"},{"key":"S002202992610257X_ref70","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.02934"},{"key":"S002202992610257X_ref51","first-page":"403","article-title":"Effects of plant extracts as pre-milking dairy cow teat sanitizer. JAPS","volume":"31","author":"Mureza","year":"2021","journal-title":"Journal of Animal and Plant Sciences"},{"key":"S002202992610257X_ref58","doi-asserted-by":"publisher","DOI":"10.1139\/cjas-2018-0173"},{"key":"S002202992610257X_ref73","doi-asserted-by":"publisher","DOI":"10.1590\/1678-4162-10681"},{"key":"S002202992610257X_ref32","first-page":"217","article-title":"Review of potential sources and control of thermoduric bacteria in bulk-tank milk","volume":"52","author":"Gleeson","year":"2013","journal-title":"Irish Journal of Agricultural and Food Research"},{"key":"S002202992610257X_ref53","unstructured":"Olanda, GB (2018) Aplica\u00e7\u00e3o Do \u00d3leo Essencial de Tagetes Minuta (Chinchilho) No Tratamento de Sementes de Feij\u00e3o [Application of the Essential Oil of Tagetes Minuta (Chinchilho) in the Treatment of Bean Seeds]. Doctoral Thesis, Federal University of Pelotas, Pelotas, Brazil."},{"key":"S002202992610257X_ref9","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/651593"},{"key":"S002202992610257X_ref27","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2015-9777"},{"key":"S002202992610257X_ref54","unstructured":"Organis - Conselho Brasileiro da Produ\u00e7\u00e3o Org\u00e2nica e Sustent\u00e1vel (2017) Consumo de Produtos Org\u00e2nicos No Brasil [Consumption of Organic Products in Brazil]. Available at: https:\/\/organis.org.br\/pesquisa-consumo-de-produtos-organicos-no-brasil-2017\/ (accessed 11 July 2024 )."},{"key":"S002202992610257X_ref22","first-page":"51","article-title":"Chemical composition of the essential oil of Tagetes minuta growing in Saudi Arabia","volume":"12","author":"El-deeb","year":"2004","journal-title":"Saudi Pharmaceutical Journal"},{"key":"S002202992610257X_ref21","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.jep.2017.06.046","article-title":"Systematic review and technological overview of the antimicrobial activity of Tagetes minuta and future perspectives","volume":"208","author":"Dos","year":"2017","journal-title":"Journal of Ethnopharmacology"},{"key":"S002202992610257X_ref36","doi-asserted-by":"publisher","DOI":"10.1186\/s12906-017-1861-6"},{"key":"S002202992610257X_ref38","doi-asserted-by":"publisher","DOI":"10.1111\/rda.13032"},{"key":"S002202992610257X_ref4","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2013.04.045"},{"key":"S002202992610257X_ref8","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.1967.tb00272.x"},{"key":"S002202992610257X_ref37","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgar.2018.04.010"},{"key":"S002202992610257X_ref23","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpharm.2017.06.052"},{"key":"S002202992610257X_ref50","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1520-6017(200005)89:5<614::AID-JPS7>3.0.CO;2-7"},{"key":"S002202992610257X_ref12","unstructured":"Bolzan, KP (2018) Desenvolvimento de Suspens\u00f5es Nanoparticuladas Com \u00d3leo Essencial de Tagetes Minuta L. E Sua Aplica\u00e7\u00e3o No Controle da Mastite Bovina. [Development of Nanoparticulate Suspensions with Essential Oil of Tagetes Minuta L. and Its Application in the Control of Bovine Mastitis]. Master's dissertation, Federal University of Santa Catarina, Florian\u00f3polis, Brazil."},{"key":"S002202992610257X_ref66","doi-asserted-by":"publisher","DOI":"10.1002\/ffj.1358"},{"key":"S002202992610257X_ref56","doi-asserted-by":"publisher","DOI":"10.3390\/nano10010135"},{"key":"S002202992610257X_ref25","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-819096-8.00060-4"},{"key":"S002202992610257X_ref69","doi-asserted-by":"publisher","DOI":"10.1002\/fsn3.85"},{"key":"S002202992610257X_ref77","doi-asserted-by":"publisher","DOI":"10.26444\/aaem\/161037"},{"key":"S002202992610257X_ref28","doi-asserted-by":"publisher","DOI":"10.1007\/s00394-013-0597-4"},{"key":"S002202992610257X_ref43","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2015-10666"},{"key":"S002202992610257X_ref67","first-page":"293","article-title":"Development of Baccharis dracunculifolia (Asteraceae) essential oil nanoemulsion and its biological activity on pre-pupae of Cochliomyia hominivorax (Diptera: calliphoridae)","volume":"7","author":"Seugling","year":"2019","journal-title":"Journal of Pharmacy and Pharmacology"},{"key":"S002202992610257X_ref10","doi-asserted-by":"publisher","DOI":"10.1079\/9781845935504.0095"},{"key":"S002202992610257X_ref7","doi-asserted-by":"publisher","DOI":"10.1111\/lam.12886"},{"key":"S002202992610257X_ref3","doi-asserted-by":"publisher","DOI":"10.1007\/s43994-022-00017-2"},{"key":"S002202992610257X_ref33","unstructured":"Gomes, FT (2017) Avalia\u00e7\u00e3o do perfil qu\u00edmico do \u00f3leo essencial de popula\u00e7\u00f5es espont\u00e2neas de Tagetes minuta L. (Asteraceae) na regi\u00e3o sul do Rio Grande do Sul. [Evaluation of the chemical profile of the essential oil of spontaneous populations of Tagetes minuta L. 21 (Asteraceae) in the southern region of Rio Grande do Sul]. Master's dissertation, Federal University of Pelotas, Pelotas, Brazil."},{"key":"S002202992610257X_ref34","unstructured":"Hemling, TC (2015) Sustainable teat disinfection for the prevention of mastitis. In Proceedings of the XVIII International Conference for Veterinarians, Polinica (Poland), pp. 23\u201328."},{"key":"S002202992610257X_ref26","doi-asserted-by":"publisher","DOI":"10.1016\/0378-5173(89)90281-0"},{"key":"S002202992610257X_ref17","first-page":"115","article-title":"Forma\u00e7\u00e3o de nanoemuls\u00f5es do tipo \u00f3leo em \u00e1gua contendo \u00f3leo de semente de rom\u00e3 [Formation of oil-in-water nanoemulsions containing pomegranate seed oil","volume":"14","author":"Silva Junior","year":"2013","journal-title":"Disciplinarum Scientia | Naturais E Tecnol\u00f3gicas"},{"key":"S002202992610257X_ref31","doi-asserted-by":"publisher","DOI":"10.3390\/molecules23061399"},{"key":"S002202992610257X_ref44","unstructured":"L\u00f3pez-benavides, M (2019) Must know facts about teat disinfectants and their role in the prevention of mastitis in modern dairy herds. 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