{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T08:31:07Z","timestamp":1789115467918,"version":"build-2803163510"},"reference-count":31,"publisher":"Cambridge University Press (CUP)","license":[{"start":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T00:00:00Z","timestamp":1789084800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Dairy Research"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    This study reports on the safety profile of\n                    <jats:italic>Loigolactobacillus coryniformis<\/jats:italic>\n                    WBB05 for food industry applications and identifies glycerol-3-phosphate oxidase (\n                    <jats:italic>GlpO<\/jats:italic>\n                    ) as a candidate gene associated with aerobic reuterin production. The safety of\n                    <jats:italic>L. coryniformis<\/jats:italic>\n                    WBB05 was evaluated through whole-genome sequencing, phenotypic analysis of haemolytic activity and determination of minimum inhibitory concentrations (MICs) of antibiotics. Comparative genomic analysis was performed to identify candidate genetic determinants for aerobic reuterin production. The draft genome (2.83 Mb, 179 contigs) harboured no known virulence factors, acquired antimicrobial resistance (AMR) genes or biogenic amine biosynthetic genes. Prophage analysis identified only one incomplete prophage region, and four CRISPR\u2013Cas systems (212 spacers) were consistent with phage defence capacity. Secondary metabolite analysis revealed biosynthetic gene clusters encoding a coagulin-like bacteriocin. No \u03b2-haemolytic activity was observed. The MICs of all antibiotics tested were below the European Food Safety Authority cut-off values except for kanamycin (128\u00a0mg\/L), although no acquired AMR genes were detected. Comparative genomic analysis revealed that\n                    <jats:italic>L. coryniformis<\/jats:italic>\n                    WBB05 possesses two putative copies of\n                    <jats:italic>GlpO<\/jats:italic>\n                    , a gene not detected in publicly available genomes of\n                    <jats:italic>Limosilactobacillus reuteri<\/jats:italic>\n                    , which produces reuterin only under anaerobic conditions. These findings support the use of\n                    <jats:italic>L. coryniformis<\/jats:italic>\n                    WBB05 as a safe adjunct culture for dairy applications and highlight\n                    <jats:italic>GlpO<\/jats:italic>\n                    as a candidate determinant of aerobic reuterin production. Further studies comparing\n                    <jats:italic>GlpO<\/jats:italic>\n                    -positive and\n                    <jats:italic>GlpO<\/jats:italic>\n                    -negative strains under aerobic and anaerobic conditions are warranted to confirm the role of\n                    <jats:italic>GlpO<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1017\/s0022029926102659","type":"journal-article","created":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T07:36:42Z","timestamp":1789112202000},"page":"1-7","source":"Crossref","is-referenced-by-count":0,"title":["Whole-genome safety assessment of\n                    <i>Loigolactobacillus coryniformis<\/i>\n                    WBB05 and identification of a candidate gene for aerobic reuterin production"],"prefix":"10.1017","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3719-4879","authenticated-orcid":false,"given":"Harutoshi","family":"Tsuda","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02syg0q74","id-type":"ROR","asserted-by":"publisher"}],"name":"Hirosaki University"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2026,9,11]]},"reference":[{"key":"S0022029926102659_ref11","first-page":"7045","article-title":"Update of the list of QPS-recommended microbiological agents intentionally added to food or feed as notified to EFSA 15: suitability of taxonomic units notified to EFSA until September 2021","volume":"20","year":"2022","journal-title":"EFSA Journal"},{"key":"S0022029926102659_ref7","doi-asserted-by":"publisher","DOI":"10.3109\/08910608909140211"},{"key":"S0022029926102659_ref12","first-page":"e9169","article-title":"Update of the list of qualified presumption of safety (QPS) recommended microbiological agents intentionally added to food or feed as notified to EFSA 21: suitability of taxonomic units notified to EFSA until September 2024","volume":"23","year":"2025","journal-title":"EFSA Journal"},{"key":"S0022029926102659_ref30","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-003-1497-y"},{"key":"S0022029926102659_ref25","doi-asserted-by":"publisher","DOI":"10.4109\/jslab1997.13.37"},{"key":"S0022029926102659_ref8","doi-asserted-by":"publisher","DOI":"10.1128\/aem.58.10.3429-3432.1992"},{"key":"S0022029926102659_ref5","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics14060612"},{"key":"S0022029926102659_ref16","first-page":"1072","article-title":"QUAST: quality assessment tool for genome assemblies","volume":"29","author":"Gurevich","year":"2013","journal-title":"Bioinformatics (Oxford, England)"},{"key":"S0022029926102659_ref10","first-page":"2740","article-title":"Guidance on the assessment of bacterial susceptibility to antimicrobials of human and veterinary importance","volume":"10","year":"2012","journal-title":"EFSA Journal"},{"key":"S0022029926102659_ref9","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.1960.tb00188.x"},{"key":"S0022029926102659_ref4","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkad344"},{"key":"S0022029926102659_ref2","doi-asserted-by":"publisher","DOI":"10.1089\/cmb.2012.0021"},{"key":"S0022029926102659_ref20","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab1107"},{"key":"S0022029926102659_ref17","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2672.1998.00466.x"},{"key":"S0022029926102659_ref22","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-019-0299-x"},{"key":"S0022029926102659_ref31","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.004107"},{"key":"S0022029926102659_ref28","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw569"},{"key":"S0022029926102659_ref6","first-page":"i884","article-title":"fastp: an ultra-fast all-in-one FASTQ preprocessor","volume":"34","author":"Chen","year":"2018","journal-title":"Bioinformatics (Oxford, England)"},{"key":"S0022029926102659_ref13","unstructured":"FAO\/WHO (2006) Probiotics in food: health and nutritional properties and guidelines for evaluation . FAO Food and Nutrition Paper 85. Rome: FAO\/WHO."},{"key":"S0022029926102659_ref19","doi-asserted-by":"publisher","DOI":"10.1080\/10408398.2011.582813"},{"key":"S0022029926102659_ref23","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2005.03.007"},{"key":"S0022029926102659_ref27","unstructured":"Seemann, T (2020) ABRicate: mass screening of contigs for antimicrobial and virulence genes. Available at https:\/\/github.com\/tseemann\/abricate (accessed 25 October 2025 )."},{"key":"S0022029926102659_ref21","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1007\/s00253-002-1099-0","article-title":"Production and stability of 3-hydroxypropionaldehyde in Lactobacillus reuteri","volume":"60","author":"L\u00fcthi-peng","year":"2002","journal-title":"Applied Microbiology and Biotechnology"},{"key":"S0022029926102659_ref24","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2017.10.037"},{"key":"S0022029926102659_ref1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw387"},{"key":"S0022029926102659_ref26","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2016-11534"},{"key":"S0022029926102659_ref3","doi-asserted-by":"publisher","DOI":"10.1126\/science.1138140"},{"key":"S0022029926102659_ref18","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmcl.2014.12.032"},{"key":"S0022029926102659_ref15","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2016.05.031"},{"key":"S0022029926102659_ref29","doi-asserted-by":"publisher","DOI":"10.1017\/S002202992300047X"},{"key":"S0022029926102659_ref14","first-page":"e00483","article-title":"Validating the AMRFinder tool and resistance gene database by using antimicrobial resistance genotype-phenotype correlations in a collection of isolates","volume":"65","author":"Feldgarden","year":"2021","journal-title":"Antimicrobial Agents and Chemotherapy"}],"container-title":["Journal of Dairy Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0022029926102659","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T07:36:44Z","timestamp":1789112204000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0022029926102659\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,11]]},"references-count":31,"alternative-id":["S0022029926102659"],"URL":"https:\/\/doi.org\/10.1017\/s0022029926102659","relation":{},"ISSN":["0022-0299","1469-7629"],"issn-type":[{"value":"0022-0299","type":"print"},{"value":"1469-7629","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,11]]}}}