{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,1,23]],"date-time":"2023-01-23T17:29:43Z","timestamp":1674494983870},"reference-count":0,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[1998,5,1]],"date-time":"1998-05-01T00:00:00Z","timestamp":893980800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Dairy Research"],"published-print":{"date-parts":[[1998,5]]},"abstract":"<jats:p>Bovine \u03b1<jats:sub>s1<\/jats:sub>-casein F (\u03b1<jats:sub>s1<\/jats:sub>-CN F)\n \nwas found in a genetic resource of \nDeutsches Schwarzbuntes Niederungsrind cows at a frequency of 0\u00b7009.\n Biochemical \ncharacterization of this new variant was obtained by automated sequencing\n of \nreversed-phase HPLC-separated tryptic peptides of \u03b1<jats:sub>s1<\/jats:sub>-CN\n F \nand \u03b1<jats:sub>s1<\/jats:sub>-CN B. \u03b1<jats:sub>s1<\/jats:sub>-CN F was found to be\n a \nsubtype of \u03b1<jats:sub>s1<\/jats:sub>-CN B with a single amino acid substitution\n \n(SerP\/Leu) in position 66. DNA sequencing revealed a C\/T transition\n in position \n8418 of the gene. Sequence-specific primers were designed to perform an\n allele-specific \npolymerase chain reaction for detection of \u03b1<jats:sub>s1<\/jats:sub>Cn<jats:sup>F<\/jats:sup>.\n \nTyping of artificial insemination sperm samples included in the genetic\n resource \nsperm pool identified one sire heterozygous for \u03b1<jats:sub>s1<\/jats:sub>Cn<jats:sup>F<\/jats:sup>.<\/jats:p>","DOI":"10.1017\/s0022029997002690","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T13:44:43Z","timestamp":1027777483000},"page":"223-231","source":"Crossref","is-referenced-by-count":3,"title":["Biochemical characterization of bovine \u03b1<sub>s1<\/sub>-casein\n F and genotyping \nwith sequence-specific primers"],"prefix":"10.1017","volume":"65","author":[{"given":"EVA-MARIA","family":"PRINZENBERG","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"PATRICIA","family":"ANGLADE","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"BRUNO","family":"RIBADEAU-DUMAS","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"GEORG","family":"ERHARDT","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[1998,5,1]]},"container-title":["Journal of Dairy Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0022029997002690","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,6]],"date-time":"2019-04-06T20:15:01Z","timestamp":1554581701000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0022029997002690\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,5]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1998,5]]}},"alternative-id":["S0022029997002690"],"URL":"https:\/\/doi.org\/10.1017\/s0022029997002690","relation":{},"ISSN":["0022-0299","1469-7629"],"issn-type":[{"value":"0022-0299","type":"print"},{"value":"1469-7629","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,5]]}}}