{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,14]],"date-time":"2026-06-14T22:07:09Z","timestamp":1781474829655,"version":"3.54.1"},"reference-count":0,"publisher":"Cambridge University Press (CUP)","issue":"1","license":[{"start":{"date-parts":[[1999,2,1]],"date-time":"1999-02-01T00:00:00Z","timestamp":917827200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Dairy Research"],"published-print":{"date-parts":[[1999,2]]},"abstract":"<jats:p>Laminitis is a major cause of lameness in dairy cattle, and is\n widely \nattributed to a defect in the horny tissue that gives the hoof its mechanical\n strength. \nDefective horn is associated with, and may be preceded by, impaired keratin \ndeposition in the hoof epidermis. The cause of abnormal keratin deposition\n is not \neasily identified but, like epidermal keratinization in other tissues,\n is likely to be \ncontrolled by hormones and the paracrine action of locally produced growth\n factors. \nThe hormonal regulation of keratin synthesis and cell proliferation in\n the bovine hoof \nwas studied using tissue explants in organ culture. As the highest incidence\n of \nlaminitis is in early lactation, the study focused on insulin, cortisol\n and prolactin, \nthree hormones implicated in lactogenesis and galactopoiesis. Incubation\n of tissue \nexplants for 24 h in medium containing insulin (10\u20135000 ng\/ml)\n stimulated protein \nsynthesis measured by incorporation of <jats:sup>35<\/jats:sup> S-labelled amino acids.\n Histochemical \nexamination showed that insulin binding co-localized with the site of protein \nsynthesis. Insulin also stimulated DNA synthesis, an index of cell proliferation, \nwhich was measured by incorporation of [<jats:sup>3<\/jats:sup>H]methyl\n thymidine. Cortisol \n(10\u20135000 ng\/ml) decreased protein synthesis, whereas prolactin\n (10\u20135000 ng\/ml) had \nno significant effect on protein or DNA synthesis. Epidermal growth factor \n(10\u2013200 ng\/ml), a potent inhibitor of keratinization in other\n tissues, stimulated \nprotein synthesis compared with untreated controls. Epidermal growth factor \nbinding was located microscopically to the germinal and differentiating\n epidermal \nlayers. SDS-PAGE and fluorography showed that the population of proteins \nsynthesized in the presence of any hormone or growth factor combination\n did not \ndiffer from that in untreated controls and included the keratins involved\n in horn \ndeposition. The results show that bovine hoof keratinization is under endocrine\n and \ngrowth factor control, and suggest that systemic changes in lactogenic\n hormones \nmay act to inhibit keratin deposition.<\/jats:p>","DOI":"10.1017\/s0022029998003288","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T13:45:41Z","timestamp":1027777541000},"page":"23-33","source":"Crossref","is-referenced-by-count":15,"title":["Effect of endocrine and paracrine factors on protein synthesis and \ncell proliferation in bovine hoof tissue culture"],"prefix":"10.1017","volume":"66","author":[{"given":"KAY A. K.","family":"HENDRY","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"AMANDA J.","family":"MacCALLUM","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"CHRISTOPHER H.","family":"KNIGHT","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"COLIN J.","family":"WILDE","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[1999,2,1]]},"container-title":["Journal of Dairy Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0022029998003288","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,4]],"date-time":"2019-04-04T21:15:17Z","timestamp":1554412517000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0022029998003288\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999,2]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1999,2]]}},"alternative-id":["S0022029998003288"],"URL":"https:\/\/doi.org\/10.1017\/s0022029998003288","relation":{},"ISSN":["0022-0299","1469-7629"],"issn-type":[{"value":"0022-0299","type":"print"},{"value":"1469-7629","type":"electronic"}],"subject":[],"published":{"date-parts":[[1999,2]]}}}