{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,5]],"date-time":"2025-10-05T19:46:10Z","timestamp":1759693570136},"reference-count":26,"publisher":"Cambridge University Press (CUP)","issue":"1","license":[{"start":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T00:00:00Z","timestamp":1244160000000},"content-version":"unspecified","delay-in-days":4114,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[1998,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>T cell populations and IL-3 mRNA expression were analysed in mesenteric lymph node cells and intestinal intraepithelial lymphocytes (IEL) in<jats:italic>Strongyloides ratti-<\/jats:italic>infected mice. On days 7 and 12 post-infection, 2.6 times as many mesenteric lymph node cells were present in<jats:italic>S. ratti-<\/jats:italic>infected mice compared with uninfected mice. Although the percentages of CD3<jats:sup>+<\/jats:sup>, CD4<jats:sup>+<\/jats:sup>and CD8<jats:sup>+<\/jats:sup>cells decreased during infection, the absolute numbers of these cell types increased on day 7 due to an overall increase in the mesenteric lymph node cell number. The CD4\/CD8 ratio in IEL was increased on day 5, whereas no significant change in the CD4\/CD8 ratio was observed in the mesenteric lymph node cells. Expression of IL-3 mRNA, which is an important cytokine for the induction of murine mucosal mastocytosis and<jats:italic>S. ratti-<\/jats:italic>expulsion, was examined in mesenteric lymph nodes and IEL of uninfected and infected mice. IL-3 mRNA was detected in mesenteric lymph nodes of<jats:italic>S. ratti<\/jats:italic>-infected mice but not detected in the lymph nodes of uninfected mice. IL-3 mRNA was detected in IEL from both infected and uninfected mice with an 20-fold increase in expression in IEL of infected mice. Overall, IL-3 mRNA levels were higher in IEL than in mesenteric lymph nodes following<jats:italic>S. ratti-<\/jats:italic>infection. Expression of IL-4, IL-10, stem cell factor (SCF or c-<jats:italic>kit<\/jats:italic>ligand) and IFN<jats:sub>-\u03b3<\/jats:sub>mRNA was also examined in these two tissues. IL-10 mRNA was not detected in any tissue examined and IFN<jats:sub>-\u03b3<\/jats:sub>mRNA levels were unaltered as a result of an<jats:italic>S. ratti-<\/jats:italic>infection. Elevated expression of mRNA for SCF (5-fold) and IL-4 (20-fold) was observed in the mesenteric lymph nodes of infected mice. In contrast, SCF mRNA levels were similar in IEL of uninfected and infected animals and only a modest increase in IL-4 mRNA was observed in IEL of infected mice.<\/jats:p>","DOI":"10.1017\/s0022149x00000894","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T14:56:20Z","timestamp":1244213780000},"page":"1-8","source":"Crossref","is-referenced-by-count":5,"title":["Analysis of T cell populations and IL-3 mRNA expression in mesenteric lymph node cells and intestinal intraepithelial lymphocytes in<i>Strongyloides ratti-<\/i>infected mice"],"prefix":"10.1017","volume":"72","author":[{"given":"T.","family":"Abe","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Sugaya","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Yoshimura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2009,6,5]]},"reference":[{"key":"S0022149X00000894_ref004","first-page":"116","article-title":"Intestinal protection against Strongyloides ratti and mastocytosis induced by administration of interleukin-in mice","volume":"80","author":"Abe","year":"1993","journal-title":"Immunology"},{"key":"S0022149X00000894_ref017","doi-asserted-by":"publisher","DOI":"10.1084\/jem.165.1.268"},{"key":"S0022149X00000894_ref005","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3024.1993.tb00578.x"},{"key":"S0022149X00000894_ref022","doi-asserted-by":"crossref","first-page":"2348","DOI":"10.4049\/jimmunol.136.7.2348","article-title":"Two types of murine helper T cell clone I. 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