{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,28]],"date-time":"2025-09-28T15:34:08Z","timestamp":1759073648411},"reference-count":16,"publisher":"Cambridge University Press (CUP)","issue":"4","license":[{"start":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T00:00:00Z","timestamp":1244160000000},"content-version":"unspecified","delay-in-days":5300,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[1994,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The enzyme-linked immunosorbent assay (ELISA) and immunoelectrophoresis (IEP) were used for the serodiagnosis of larval<jats:italic>Anisakis simplex<\/jats:italic>infections in man and immunized rabbits. Sephacryl S-300 gel filtration was used for separating crude antigen. Four fractions were obtained. Sera from patients with other helminth infections sometimes cross reacted with<jats:italic>Anisakis<\/jats:italic>larval antigens. With IEP, crude antigen is more sensitive than purified antigens. With ELISA, the third fraction is the most sensitive for detecting antibodies to<jats:italic>Anisakis<\/jats:italic>larvae in the sera of humans and immunized rabbits.<\/jats:p>","DOI":"10.1017\/s0022149x0000153x","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T15:09:30Z","timestamp":1244214570000},"page":"305-309","source":"Crossref","is-referenced-by-count":4,"title":["Detection of antibodies to<i>Anisakis simplex<\/i>larvae by enzyme-linked immunosorbent assay and immunoelectrophoresis using crude or purified antigens"],"prefix":"10.1017","volume":"68","author":[{"given":"R. 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