{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,4,2]],"date-time":"2022-04-02T18:56:59Z","timestamp":1648925819457},"reference-count":14,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T00:00:00Z","timestamp":1244160000000},"content-version":"unspecified","delay-in-days":10596,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[1980,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The indirect fluorescent antibody technique has been applied to detect antibody levels in jirds (<jats:italic>meriones unguiculatus<\/jats:italic>) infected with <jats:italic>Brugia malayi<\/jats:italic>. Sonicated antigens were prepared from microfilariae and adult worms. Sonicated microfilariae were found to be satisfactory for this purpose. Cyanogen bromide-activated sepharose coated with soluble antigens prepared from microfilariae and adult worms was also used to detect antibodies to <jats:italic>Brugia<\/jats:italic> infections. The present observation show that these techniques can be usefully applied for detection of filarial infections. Antibody titres in infected jirds generally ranged from 1: 16 \u20131:256 and were not affected by treatment with diethylcarbamazine.<\/jats:p>","DOI":"10.1017\/s0022149x00006490","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T15:33:22Z","timestamp":1244216002000},"page":"147-153","source":"Crossref","is-referenced-by-count":5,"title":["Immunofluorescent studies in filariasis: antibody levels in jirds (<i>Meriones unguiculatus<\/i>) infected with <i>Brugia malayi<\/i>"],"prefix":"10.1017","volume":"54","author":[{"given":"Mulkit","family":"Singh","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. J.","family":"Kane","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Burren","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. M.","family":"Mackinlay","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2009,6,5]]},"reference":[{"key":"S0022149X00006490_ref013","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(69)90123-0"},{"key":"S0022149X00006490_ref011","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(75)90118-0"},{"key":"S0022149X00006490_ref010","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(01)81233-4"},{"key":"S0022149X00006490_ref008","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(77)90105-5"},{"key":"S0022149X00006490_ref006","doi-asserted-by":"publisher","DOI":"10.2307\/3277959"},{"key":"S0022149X00006490_ref003","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(74)90067-2"},{"key":"S0022149X00006490_ref002","doi-asserted-by":"publisher","DOI":"10.2307\/3277515"},{"key":"S0022149X00006490_ref014","first-page":"493","article-title":"Studies on immunization against Brugia malayi infection in the rhesus monkey","volume":"40","author":"Wong","year":"1969","journal-title":"Bulletin of the World Health Organization"},{"key":"S0022149X00006490_ref007","first-page":"184","article-title":"Worm burdens and prepatent periods in jirds (Meriones unguiculatus) infected with Brugia malayi","volume":"4","author":"El Bihari","year":"1973","journal-title":"Southeast Asian Journal of Tropical Medicine and Public Health"},{"key":"S0022149X00006490_ref001","unstructured":"Ash L. R. (1974) Rodent models for the study of lymphatic-dwelling filarial worms. WHO mimeographed document WHO\/FIL\/74.121 (unpublished)."},{"key":"S0022149X00006490_ref004","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-308X(08)60530-8"},{"key":"S0022149X00006490_ref012","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(78)90209-2"},{"key":"S0022149X00006490_ref005","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(58)90112-3"},{"key":"S0022149X00006490_ref009","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1971.tb35040.x"}],"container-title":["Journal of Helminthology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0022149X00006490","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,5,26]],"date-time":"2019-05-26T19:10:14Z","timestamp":1558897814000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0022149X00006490\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1980,6]]},"references-count":14,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1980,6]]}},"alternative-id":["S0022149X00006490"],"URL":"https:\/\/doi.org\/10.1017\/s0022149x00006490","relation":{},"ISSN":["0022-149X","1475-2697"],"issn-type":[{"value":"0022-149X","type":"print"},{"value":"1475-2697","type":"electronic"}],"subject":[],"published":{"date-parts":[[1980,6]]}}}