{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T18:23:11Z","timestamp":1792002191506,"version":"4.4.0"},"reference-count":15,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T00:00:00Z","timestamp":1244160000000},"content-version":"unspecified","delay-in-days":8405,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[1986,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The pathogenesis of unirradiated, 3 krad-irradiated and 20 krad-irradiated metacercarial infections was compared in zebu calves studied over a 10-week period. Calves exposed to 1000 unirradiated metacercariae (mc) became hypoalbuminaemic, and showed elevated serum concentrations of liver enzymes, whereas neither of the other groups was significantly affected. At slaughter, a mean of 332 flukes was recovered from the 0 krad group, while only 23% and 12% of this number were recovered from the 3 krad and 20 krad groups, respectively. All the worms recovered from the 20 krad group were stunted, and found in biliary ductules, but a mean of 13% of the flukes recovered from the 3 krad group were large, and dwelling in main bile-ducts. Liver lesions typical of acute fascioliasis were present in the 0 krad group, but lesions in the other groups, and particularly the 20 krad group, were far less severe. Judged on clinico-pathological criteria, a single vaccination of calves with 1000 3 krad-irradiated mc induced partial resistance to a challenge with 1000 normal mc eight weeks later, but the reduction in worm recovery was not statistically significant. There was less evidence of protection when two vaccinating doses of 3 krad mc were given within four weeks, with challenge at week 8, and a single vaccination was ineffective aganist a challenge four weeks later. However, when the irradiation dose was increased to 20 krad, a high level of resistance (69% worm reduction) was induced by a single vaccination, given eight weeks before challenge, and liver pathology was strikingly reduced in the vaccinated animals.<\/jats:p>","DOI":"10.1017\/s0022149x0000835x","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T15:23:24Z","timestamp":1244215404000},"page":"123-134","source":"Crossref","is-referenced-by-count":7,"title":["Immunization of zebu calves against<i>Fasciola gigantica<\/i>, using irradiated metacercariae"],"prefix":"10.1017","volume":"60","author":[{"given":"S. A.","family":"Younis","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. I.","family":"Yagi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E. M.","family":"Haroun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. A.","family":"Gameel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. G.","family":"Taylor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2009,6,5]]},"reference":[{"key":"S0022149X0000835X_ref015","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/16.3_ts.40"},{"key":"S0022149X0000835X_ref012","doi-asserted-by":"publisher","DOI":"10.1016\/S0007-1935(17)36487-4"},{"key":"S0022149X0000835X_ref011","first-page":"577","article-title":"Serum enzyme activity in acute ovine fascioliasis","volume":"80","author":"Sewell","year":"1967","journal-title":"Veterinary Record"},{"key":"S0022149X0000835X_ref010","doi-asserted-by":"publisher","DOI":"10.1136\/vr.78.3.98"},{"key":"S0022149X0000835X_ref008","doi-asserted-by":"publisher","DOI":"10.1016\/0020-708X(80)90146-5"},{"key":"S0022149X0000835X_ref014","first-page":"115","article-title":"Vaccination against trematodes","volume":"18","author":"Taylor","year":"1980","journal-title":"\u201cVaccination against Parasites\u201d, British Society for Parasitology Symposium"},{"key":"S0022149X0000835X_ref007","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1016\/S0034-5288(18)33502-1","article-title":"Resistance in cattle to Fasciola hepatica induced by X-ray attenuated larvae: results from a controlled field trial","volume":"19","author":"Nansen","year":"1975","journal-title":"Research in Veterinary Science"},{"key":"S0022149X0000835X_ref004","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1016\/S0034-5288(18)34899-9","article-title":"The comparative activity of serum enzymes in sheep, cattle and rat in normal serum and tissue levels and changes during experimental liver necrosis","volume":"3","author":"Boyd","year":"1962","journal-title":"Research in Veterinary Science"},{"key":"S0022149X0000835X_ref003","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-308X(08)60435-2"},{"key":"S0022149X0000835X_ref002","first-page":"23","volume-title":"Isotopes and Radiation in Parasitology","author":"Bitakaramire","year":"1973"},{"key":"S0022149X0000835X_ref005","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9975(67)90025-4"},{"key":"S0022149X0000835X_ref013","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1016\/S0034-5288(18)33506-9","article-title":"Resistance of sheep to F. hepatica","volume":"19","author":"Sinclair","year":"1975","journal-title":"Research in Veterinary Science"},{"key":"S0022149X0000835X_ref006","doi-asserted-by":"publisher","DOI":"10.1002\/path.1700830106"},{"key":"S0022149X0000835X_ref001","first-page":"494","article-title":"Immunisation against fascioliasis","volume":"1","author":"Armour","year":"1974","journal-title":"Proceedings of the Third International Congress of Parasitology, Munich"},{"key":"S0022149X0000835X_ref009","doi-asserted-by":"crossref","first-page":"478","DOI":"10.1093\/clinchem\/11.4.478","article-title":"Direct spectrophotometric determination of albumin in human serum","volume":"11","author":"Rodkey","year":"1965","journal-title":"Clinical Chemistry"}],"container-title":["Journal of Helminthology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0022149X0000835X","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,19]],"date-time":"2020-05-19T06:57:40Z","timestamp":1589871460000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0022149X0000835X\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1986,6]]},"references-count":15,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1986,6]]}},"alternative-id":["S0022149X0000835X"],"URL":"https:\/\/doi.org\/10.1017\/s0022149x0000835x","relation":{},"ISSN":["0022-149X","1475-2697"],"issn-type":[{"value":"0022-149X","type":"print"},{"value":"1475-2697","type":"electronic"}],"subject":[],"published":{"date-parts":[[1986,6]]}}}