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The molecular weight of ECF was estimated to be 25 000\u201345 000 by high-pressure liquid chromatography. The ECF was bound with concanavalin A-Sepharose. The ECF was sensitive to periodate oxidation and to heating (56\u00b0C, 30 min). On isoelectric focusing, eosinophil chemotactic activity was clearly revealed at pI 4\u00b71. These results suggest that ECF of<jats:italic>S. erinacei<\/jats:italic>plerocercoid is an acidic glycoprotein. An intradermal injection of ECF induced eosinophil attractions in the normal guinea pig skin.<\/jats:p>","DOI":"10.1017\/s0022149x00008646","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T15:19:18Z","timestamp":1244215158000},"page":"6-12","source":"Crossref","is-referenced-by-count":5,"title":["Leukocyte accumulation in sparganosis: further characterization of an eosinophil chemotactic factor of the plerocercoid of<i>Spirometra erinacei<\/i>"],"prefix":"10.1017","volume":"63","author":[{"given":"Y.","family":"Horii","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Owhashi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Ishii","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Fujita","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2009,6,5]]},"reference":[{"key":"S0022149X00008646_ref015","doi-asserted-by":"crossref","first-page":"302","DOI":"10.4049\/jimmunol.122.1.302","article-title":"Ascaris and eosinophil. 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