{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T20:28:37Z","timestamp":1791404917308,"version":"4.3.3"},"reference-count":28,"publisher":"Cambridge University Press (CUP)","issue":"3","license":[{"start":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T00:00:00Z","timestamp":1244160000000},"content-version":"unspecified","delay-in-days":7217,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[1989,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Infective larvae of <jats:italic>Onchocerca lienalis<\/jats:italic> and <jats:italic>O.volvulus<\/jats:italic> implanted subcutaneously within micropore chambers into laboratory hosts moulted to the fourth stage (L4) and underwent limited development and growth. Similar recoveries of <jats:italic>O. lienalis<\/jats:italic> L4 larvae in the range of 33\u201366% were obtained from chambers implanted into CBA and BALB\/c strains of mice, jirds, and the natural bovine host. A relatively constant proportion of larvae survived up to 24 days post implantation and thereafter recoveries declined, although some worms were still alive after 96 days. Recoveries of <jats:italic>O. volvulus<\/jats:italic> L4 larvae from chambers given to normal or T-cell deprived mice were equivalent to one another and to those obtained with <jats:italic>O. lienalis<\/jats:italic>. Moulting of <jats:italic>O. lienalis<\/jats:italic> in chambers was observed on days 3 and 5, in close accordance with the timing of the third moult in cattle following systemic infection. Moulting of <jats:italic>O. volvulus<\/jats:italic> occurred between days 3\u20136. Morphological changes in developing larvae included a small but significant increase in length, a transient increase in width, and early development of the spicular primordia and genital tube. L4 larvae of <jats:italic>O. lienalis<\/jats:italic>, but not those of <jats:italic>O. volvulus<\/jats:italic>, exhibited 3 distinct caudal papillae not present on infective larvae.<\/jats:p>","DOI":"10.1017\/s0022149x00009019","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T11:17:42Z","timestamp":1244200662000},"page":"218-226","source":"Crossref","is-referenced-by-count":21,"title":["Fate of developing larvae of <i>Onchocerca lienalis<\/i> and <i>O.volvulus<\/i> in micropore chambers implanted into laboratory hosts"],"prefix":"10.1017","volume":"63","author":[{"given":"A. E.","family":"Bianco","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. B.","family":"Mustafa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. J.","family":"Ham","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2009,6,5]]},"reference":[{"key":"S0022149X00009019_ref023","first-page":"120","article-title":"Development of infective larvae of Onchocerca volvulus in diffusion chambers implanted into Mastomys natalensis","volume":"36","author":"Strote","year":"1985","journal-title":"Tropenmedizin und Parasitologie"},{"key":"S0022149X00009019_ref028","first-page":"73","article-title":"Studies on Dipetalonema viteae (Filarioidea). 3. Antibody dependent cell mediated destruction of microfilariae in vivo","volume":"30","author":"Weiss","year":"1979","journal-title":"Tropenmedizin und Parasitologie"},{"key":"S0022149X00009019_ref006","unstructured":"Bianco A. E. & Muller R. (1982) Experimental transmission of Onchocerca lienalis to calves. In: Parasites \u2013 Their World and Ours. Proceedings of the Fifth International Congress of Parasitology, August 7\u201314, Toronto, 1982. 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