{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T04:18:53Z","timestamp":1768709933822,"version":"3.49.0"},"reference-count":27,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T00:00:00Z","timestamp":1244160000000},"content-version":"unspecified","delay-in-days":8040,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[1987,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Antibodies directed against diagnostic M<jats:sub>r<\/jats:sub>31 000 polypeptide(s) of adult<jats:italic>Schistosoma mansoni<\/jats:italic>were already formed in mice during prepatency. In contrast, repeated immunization of mice with homogenates of adult schistosomes failed to elicit antibodies detectable in immunoblots in the M<jats:sub>r<\/jats:sub>31 000 region. Therefore, spleen cells of infected mice were used to produce hybridoma lines. The \u201cminiblot technique\u201d was developed in order to detect in hybridoma supernatants antibodies against schistosome M<jats:sub>r<\/jats:sub>31 000 components. Electrophoretically separated total<jats:italic>S. mansoni<\/jats:italic>proteins were transferred onto nitrocellulose, and the position of the M<jats:sub>r<\/jats:sub>31 000 components was determined with polyclonal antisera and immunoblotting. Pieces of about 3 square mm of nitrocellulose bearing the diagnostic proteins were incubated with about 100 \u03bc1 of hybridoma supernatant in microtitre plates and subsequently probed with peroxidase-conjugated antibody to mouse IgG. This screening technique identified hybridomas secreting antibody to the relevant<jats:italic>S. mansoni<\/jats:italic>antigens. It is applicable to other defined parasit antigens, which are, however, not available in biochemically purified form. The monoclonal antibodies produced against the proteins with diagnostic potential reacted with antigens localized in the schistosome gut.<\/jats:p>","DOI":"10.1017\/s0022149x00009810","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T15:22:10Z","timestamp":1244215330000},"page":"95-101","source":"Crossref","is-referenced-by-count":22,"title":["Diagnostic M<sub>r<\/sub>31 000<i>Schistosoma mansoni<\/i>proteins: requirement of infection, but not immunization, and use of the \u201cminiblot\u201d technique for the production of monoclonal antibodies"],"prefix":"10.1017","volume":"61","author":[{"given":"Andreas","family":"Ruppel","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ute","family":"Breternitz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reinhard","family":"Burger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2009,6,5]]},"reference":[{"key":"S0022149X00009810_ref008","first-page":"91","article-title":"Purification studies on an acidic protease from adult Schistosoma mansoni","volume":"45","author":"Deelder","year":"1977","journal-title":"Acta Leidensia"},{"key":"S0022149X00009810_ref025","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1981.30.96"},{"key":"S0022149X00009810_ref013","first-page":"729","article-title":"Collaborative study on antigens for immunodiagnosis of schistosomiasis","volume":"60","author":"Mott","year":"1982","journal-title":"Bulletin of the World Health Organization"},{"key":"S0022149X00009810_ref018","first-page":"351","volume-title":"Selected methods in cellular immunology","author":"Oi","year":"1980"},{"key":"S0022149X00009810_ref007","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(80)90004-1"},{"key":"S0022149X00009810_ref001","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(82)90089-8"},{"key":"S0022149X00009810_ref010","doi-asserted-by":"crossref","first-page":"1548","DOI":"10.4049\/jimmunol.123.4.1548","article-title":"A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody-secreting hybrid cell lines","volume":"123","author":"Kearney","year":"1979","journal-title":"Journal of Immunology"},{"key":"S0022149X00009810_ref022","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000055293"},{"key":"S0022149X00009810_ref012","first-page":"257","volume-title":"Parasite antigens","author":"Mitchell","year":"1986"},{"key":"S0022149X00009810_ref014","first-page":"938","article-title":"Antibody response to a polysaccharide antigen present in the schistosome gut. I Sensitivity and specificity","volume":"27","author":"Nash","year":"1978","journal-title":"American Journal of Tropical Medicine and Hygiene"},{"key":"S0022149X00009810_ref024","first-page":"205","article-title":"Properties of a proteolytic enzyme from Schistosoma mansoni","volume":"42B","author":"Sauer","year":"1972","journal-title":"Comparative Biochemistry and Parasitology"},{"key":"S0022149X00009810_ref026","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000086248"},{"key":"S0022149X00009810_ref016","doi-asserted-by":"crossref","first-page":"805","DOI":"10.4049\/jimmunol.126.2.805","article-title":"Analysis and antigenic activity of a carbohyrate fraction derived from adult Schistosoma mansoni","volume":"126","author":"Nash","year":"1981","journal-title":"Journal of Immunology"},{"key":"S0022149X00009810_ref023","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(85)90023-2"},{"key":"S0022149X00009810_ref021","first-page":"499","article-title":"Immunoblot analysis of Schistosoma mansoni antigens with sera of schistosomiasis patients: diagnostic potential of an adult schistosome polypeptide","volume":"62","author":"Ruppel","year":"1985","journal-title":"Clinical and Experimental Immunology"},{"key":"S0022149X00009810_ref017","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(84)90070-5"},{"key":"S0022149X00009810_ref027","first-page":"68","article-title":"Studies of a proteolytic enzyme from Schistosoma mansoni","volume":"14","author":"Timms","year":"1959","journal-title":"British Journal of Pharmacology"},{"key":"S0022149X00009810_ref005","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(83)90033-1"},{"key":"S0022149X00009810_ref009","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(82)90677-7"},{"key":"S0022149X00009810_ref002","doi-asserted-by":"crossref","first-page":"2042","DOI":"10.4049\/jimmunol.129.5.2042","article-title":"Identification of functionally relevant determinants on the complement component C3 with monoclonal antibodies","volume":"129","author":"Burger","year":"1982","journal-title":"Journal of Immunology"},{"key":"S0022149X00009810_ref003","doi-asserted-by":"crossref","first-page":"2442","DOI":"10.4049\/jimmunol.124.5.2442","article-title":"Purification immunochemical, and biologic characterization of the Schistosoma circulating M antigen","volume":"124","author":"Carlier","year":"1980","journal-title":"Journal of Immunology"},{"key":"S0022149X00009810_ref004","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(86)90148-7"},{"key":"S0022149X00009810_ref020","first-page":"76","article-title":"Schistosoma mansoni: Characterization of a protein with diagnostic potential using monospecific patient sera and monoclonal antibodies","volume":"170","author":"Ruppel","year":"1985","journal-title":"Immunobiology"},{"key":"S0022149X00009810_ref019","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(78)90155-8"},{"key":"S0022149X00009810_ref006","first-page":"36","article-title":"Immunodiagnosis of recently acquired Schistosoma mansoni infection. A comparison of various immunological techniques","volume":"33","author":"Deelder","year":"1981","journal-title":"Tropical and Geographical Medicine"},{"key":"S0022149X00009810_ref015","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1985.34.236"},{"key":"S0022149X00009810_ref011","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.75.11.5715"}],"container-title":["Journal of Helminthology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0022149X00009810","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,3,14]],"date-time":"2024-03-14T09:09:05Z","timestamp":1710407345000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0022149X00009810\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1987,6]]},"references-count":27,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1987,6]]}},"alternative-id":["S0022149X00009810"],"URL":"https:\/\/doi.org\/10.1017\/s0022149x00009810","relation":{},"ISSN":["0022-149X","1475-2697"],"issn-type":[{"value":"0022-149X","type":"print"},{"value":"1475-2697","type":"electronic"}],"subject":[],"published":{"date-parts":[[1987,6]]}}}