{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,17]],"date-time":"2023-10-17T10:30:27Z","timestamp":1697538627076},"reference-count":17,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T00:00:00Z","timestamp":1244160000000},"content-version":"unspecified","delay-in-days":6579,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[1991,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The existence of adult<jats:italic>Schistosoma haematobium<\/jats:italic>worm proteins (Sh31\/32) homologous to the diagnostic Sm31\/32 of<jats:italic>S. mansoni<\/jats:italic>is shown. Sm31\/32 and Sh31\/32, adsorbed on nitrocellulose and kept dry on the bench before immunoblot analysis, were antigenically stable for at least 4 years including storage for 17 months in tropical climates. The antigens react with patient sera in the absence of defined buffers under rather simple conditions (\u201chumid chamber blot\u201d). The results add to the use of these antigens for serodiagnosis of schistosomiasis in endemic areas.<\/jats:p>","DOI":"10.1017\/s0022149x0001052x","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T15:15:03Z","timestamp":1244214903000},"page":"89-94","source":"Crossref","is-referenced-by-count":2,"title":["Diagnostic Mr 31\/32000 proteins of<i>Schistosoma mansoni<\/i>(Sm31\/32) and<i>S. haematobium<\/i>(Sh31\/32): stability and reaction conditions for prospective field tests"],"prefix":"10.1017","volume":"65","author":[{"given":"Mohamed A.","family":"Idris","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andreas","family":"Ruppel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2009,6,5]]},"reference":[{"key":"S0022149X0001052X_ref010","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(89)90025-X"},{"key":"S0022149X0001052X_ref008","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X00011305"},{"key":"S0022149X0001052X_ref006","doi-asserted-by":"crossref","first-page":"766","DOI":"10.4049\/jimmunol.116.3.766","article-title":"Visual detection of carcinoembryonic antigen on surfaces","volume":"116","author":"Giaever","year":"1976","journal-title":"Journal of Immunology"},{"key":"S0022149X0001052X_ref004","doi-asserted-by":"crossref","first-page":"12851","DOI":"10.1016\/S0021-9258(18)45284-2","article-title":"Cloning and gene expression of Schistosoma mansoni protease","volume":"262","author":"Davis","year":"1987","journal-title":"Journal of Biological Chemistry"},{"key":"S0022149X0001052X_ref009","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(87)90363-4"},{"key":"S0022149X0001052X_ref003","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1990.42.335"},{"key":"S0022149X0001052X_ref001","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(87)90613-8"},{"key":"S0022149X0001052X_ref002","first-page":"309","volume-title":"Molecular Strategies of Parasite Invasion","author":"Chappell","year":"1987"},{"key":"S0022149X0001052X_ref015","first-page":"127","article-title":"Schistosoma mansoni diagnostic antigens (Sm 31\/32): a seroepidemiological study in the Sudan","volume":"41","author":"Ruppel","year":"1990","journal-title":"Tropical Medicine and Parasitology"},{"key":"S0022149X0001052X_ref011","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(85)90368-2"},{"key":"S0022149X0001052X_ref005","doi-asserted-by":"publisher","DOI":"10.1080\/00034983.1988.11812246"},{"key":"S0022149X0001052X_ref012","first-page":"11","article-title":"Aspects of simplified methods for serodiagnostic use in field work","volume":"62","author":"Nilsson","year":"1985","journal-title":"Developments in Biological Standardization"},{"key":"S0022149X0001052X_ref013","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X00009810"},{"key":"S0022149X0001052X_ref017","first-page":"245","article-title":"Diagnostic 31\/32 kD schistosome proteins in Schistosoma japonicum: reactivity in immunoblots with patient sera and monoclonal antibodies","volume":"6","author":"Shi","year":"1988","journal-title":"Chinese Journal of Parasitology and Parasitic Diseases"},{"key":"S0022149X0001052X_ref014","first-page":"499","article-title":"Immunoblot analysis of Schistosoma mansoni antigens with sera of schistosomiasis patients: diagnostic potential of an adult schistosome polypeptide","volume":"62","author":"Ruppel","year":"1985","journal-title":"Clinical and Experimental Immunology"},{"key":"S0022149X0001052X_ref007","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(86)90162-6"},{"key":"S0022149X0001052X_ref016","first-page":"291","article-title":"Sera of Schistosoma japonicum-infected patients cross-react with diagnostic 31\/32 kD proteins of S. mansoni","volume":"69","author":"Ruppel","year":"1987","journal-title":"Clinical and Experimental Immunology"}],"container-title":["Journal of Helminthology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0022149X0001052X","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,25]],"date-time":"2023-05-25T14:33:35Z","timestamp":1685025215000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0022149X0001052X\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,6]]},"references-count":17,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1991,6]]}},"alternative-id":["S0022149X0001052X"],"URL":"https:\/\/doi.org\/10.1017\/s0022149x0001052x","relation":{},"ISSN":["0022-149X","1475-2697"],"issn-type":[{"value":"0022-149X","type":"print"},{"value":"1475-2697","type":"electronic"}],"subject":[],"published":{"date-parts":[[1991,6]]}}}