{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,14]],"date-time":"2026-11-14T09:46:52Z","timestamp":1794649612082,"version":"4.9.1"},"reference-count":13,"publisher":"Cambridge University Press (CUP)","issue":"1","license":[{"start":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T00:00:00Z","timestamp":1244160000000},"content-version":"unspecified","delay-in-days":7766,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[1988,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Transplanted infections of <jats:italic>Dipetalonema viteae and Brugia pahangi<\/jats:italic> have been evaluated as tools for experimental chemotherapy. Attempts were made to establish these filariae in similar pharmacokinetic sites within the same host, so that direct comparisons of <jats:italic>in vivo<\/jats:italic> drug susceptibilities could be made. Unfortunately, it was not possible to establish <jats:italic>B. pahangi<\/jats:italic> in the subcutaneous tissues, the preferred site of <jats:italic>D. viteae<\/jats:italic>. Therefore, intraperitoneal <jats:italic>B. pahangi<\/jats:italic> and subcutaneously implanted <jats:italic>D. viteae<\/jats:italic> in gerbils were used for the study. <jats:italic>D. viteae<\/jats:italic> infections were significantly enhanced by concomitant infections with <jats:italic>B. pahangi<\/jats:italic>, while <jats:italic>B. pahangi<\/jats:italic> infection rates were unaffected by the presence of <jats:italic>D. viteae<\/jats:italic>. Experiments with amoscanate, CGP6140 and Mel W demonstrated the importance of employing both <jats:italic>B. pahangi<\/jats:italic> and <jats:italic>D. viteae<\/jats:italic> for antifilarial discovery work and the fundamental effect of parasite location on drug efficacy. <jats:italic>D. viteae<\/jats:italic> rapidly migrate from the peritoneal cavity of gerbils following implantation; twenty one hours after infection 73% of transplanted worms were found in the subcutaneous tissues. It was shown that the migration response could be used as a stringent parameter for demonstrating antifilarial activity. <jats:italic>D. viteae<\/jats:italic> were exposed to antifilarial drugs for 24 hours <jats:italic>in vitro<\/jats:italic>, washed and implanted into the peritoneal cavity of gerbils. At autopsy, 5 days later, 10<jats:sup>\u22128<\/jats:sup>M ivermectin and milbemycin D had prevented migration; CGP6140, amoscanate, suramin, flubendazole and furapyrimidone were also detected at &lt;10<jats:sup>\u22126<\/jats:sup>M using this parameter. In all cases the migration response was more sensitive to drugs than parasite kill. Ivermectin's ability to inhibit worm migration through the tissues is discussed, with respect to the role of itinerant males in the reproductive cycle of <jats:italic>Onchocerca volvulus<\/jats:italic>.<\/jats:p>","DOI":"10.1017\/s0022149x00011123","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T11:20:54Z","timestamp":1244200854000},"page":"1-9","source":"Crossref","is-referenced-by-count":8,"title":["<i>Dipetalonema viteae<\/i> and <i>Brugia pahangi<\/i> transplant infections in gerbils for use in antifilarial screening"],"prefix":"10.1017","volume":"62","author":[{"given":"J. P.","family":"Court","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. N.","family":"Stables","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G. M.","family":"Lees","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. R.","family":"Martin-Short","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Rankin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2009,6,5]]},"reference":[{"key":"S0022149X00011123_ref011","first-page":"750","article-title":"Untersuchungen uber eine immunitat gegen microfilarien der rt Dipetalonema viteae bei Meriones libycus und beim hamster","volume":"76","author":"Weiss","year":"1969","journal-title":"Revue Suisse de Zoologie"},{"key":"S0022149X00011123_ref010","first-page":"90","article-title":"Dipetalonema viteae infected Mongolian jirds as a routine model for screening potential antifilarial compounds","volume":"37","author":"Striebel","year":"1986","journal-title":"Tropical Medicine and Parasitology"},{"key":"S0022149X00011123_ref004","doi-asserted-by":"publisher","DOI":"10.2307\/3278561"},{"key":"S0022149X00011123_ref009","first-page":"72","article-title":"Development of an adult Dipetalonema viteae in vitro\/in vivo migration assay for detecting antifilarial activity","volume":"38","author":"Stables","year":"1987","journal-title":"Tropical Medicine and Parasitology"},{"key":"S0022149X00011123_ref003","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000052392"},{"key":"S0022149X00011123_ref001","first-page":"357","article-title":"A comparison of the response of Dipetalonema viteae and Brugia pahangi adult worms to antifilarial agents in vitro","volume":"37","author":"Court","year":"1986","journal-title":"Tropical Medicine and Parasitology"},{"key":"S0022149X00011123_ref007","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(76)90112-0"},{"key":"S0022149X00011123_ref005","first-page":"137","article-title":"Migration of adult worms of Dipetalonema viteae subcutaneously transplanted into the jird","volume":"32","author":"Nogami","year":"1983","journal-title":"Japanese Journal of Parasitology"},{"key":"S0022149X00011123_ref006","first-page":"413","article-title":"Specific cellular immune unresponsiveness in human filariases","volume":"33","author":"Ottesen","year":"1977","journal-title":"Immunology"},{"key":"S0022149X00011123_ref013","unstructured":"World Health Organization (1985) Report on the eleventh meeting of the Scientific Working Group on Filariasis on review of filaricide screening methods and reults in relation to synthetic chemistry, biochemistry and toxicology held jointly with the Onchocerciasis Chemotherapy Project, TDR\/FIL-SWG (11)\/85.3 (Mimeo)."},{"key":"S0022149X00011123_ref012","first-page":"73","article-title":"Studies on Dipetalonema viteae (Filaroidea). 3. Antibody dependent cell-mediated destruction of microfilariae in vivo","volume":"30","author":"Weiss","year":"1979","journal-title":"Tropenmedizin und Parasitologie"},{"key":"S0022149X00011123_ref002","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(79)90018-X"},{"key":"S0022149X00011123_ref008","doi-asserted-by":"publisher","DOI":"10.1016\/0169-4758(86)90138-9"}],"container-title":["Journal of Helminthology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0022149X00011123","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,5,20]],"date-time":"2019-05-20T16:12:42Z","timestamp":1558368762000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0022149X00011123\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1988,3]]},"references-count":13,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1988,3]]}},"alternative-id":["S0022149X00011123"],"URL":"https:\/\/doi.org\/10.1017\/s0022149x00011123","relation":{},"ISSN":["0022-149X","1475-2697"],"issn-type":[{"value":"0022-149X","type":"print"},{"value":"1475-2697","type":"electronic"}],"subject":[],"published":{"date-parts":[[1988,3]]}}}