{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,3,14]],"date-time":"2024-03-14T09:42:25Z","timestamp":1710409345894},"reference-count":28,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T00:00:00Z","timestamp":1244160000000},"content-version":"unspecified","delay-in-days":6944,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[1990,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The surface antigens of the bovine filarial parasite<jats:italic>Setaria digitata<\/jats:italic>were isolated by EDTA extraction and purified by affinity chromatography using sepharose bound human filarial (<jats:italic>Wuchereria bancrofti<\/jats:italic>) antibodies obtained from chronic human filarial sera. The purified and crude antigens were used in enzyme-linked immunosorbent assay (ELISA) for the detection of serum antibodies in bancroftian filariasis. The purified antigen showed sensitive and specific reactions in ELISA for the detection of antibodies in filarial sera and showed least cross reactivity with other parasitic infections. The crude and purified antigens showed about 18 and 6 peptide bands respectively in SDS-PAGE and about 11 and 6 antigenic bands respectively in enzyme-linked immunoelectrotransfer blot (EITB). The purified antigen was observed to be glycoprotein in nature. It was possible to identify the stage-specific infection in human filariasis by using the crude and purified antigens in EITB.<\/jats:p>","DOI":"10.1017\/s0022149x00012001","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T15:15:42Z","timestamp":1244214942000},"page":"105-114","source":"Crossref","is-referenced-by-count":11,"title":["Isolation, purification and characterization of surface antigens of the bovine filarial parasite<i>Setaria digitata<\/i>for the immunodiagnosis of bancroftian filariasis"],"prefix":"10.1017","volume":"64","author":[{"given":"J. Gerald","family":"Theodore","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Kaliraj","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2009,6,5]]},"reference":[{"key":"S0022149X00012001_ref021","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X00027103"},{"key":"S0022149X00012001_ref028","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(83)92032-3"},{"key":"S0022149X00012001_ref016","first-page":"269","article-title":"Antigenic analysis of Brugia timori, a filarial nematode of man: Initial characterization by surface radioiodination and evaluation of diagnostic potential","volume":"51","author":"Maizels","year":"1983","journal-title":"Clinical and Experimental Immunology"},{"key":"S0022149X00012001_ref026","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(63)90041-9"},{"key":"S0022149X00012001_ref014","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"S0022149X00012001_ref013","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1111\/j.1749-6632.1963.tb40661.x","article-title":"Comparative immunochemistry of farval and adult worms of Schistosoma mansoni","volume":"133","author":"Kent","year":"1963","journal-title":"Annals of the New York Academy of Sciences"},{"key":"S0022149X00012001_ref010","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1017\/S0022149X00025608","article-title":"The utility of human filarial serum in the detection of circulating antigen","volume":"55","author":"Kaliraj","year":"1981","journal-title":"Journal of Helminthology"},{"key":"S0022149X00012001_ref006","first-page":"343","article-title":"The major radioiodinated cuticular antigens of Onchocerca gibsoni microfilariae are neither species nor Onchocerca specific","volume":"38","author":"Forsyth","year":"1981","journal-title":"Acta Tropica"},{"key":"S0022149X00012001_ref005","first-page":"649","article-title":"Antigens of Setaria digitata: Cross reaction with surface antigens of Wuchereria bancrofti microfilariae and serum antibodies in W. bancrofti infected subjects","volume":"58","author":"Dissanayake","year":"1980","journal-title":"Bulletin of the World Health Organization"},{"key":"S0022149X00012001_ref027","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.9.4350"},{"key":"S0022149X00012001_ref004","first-page":"30","article-title":"Litmosoides curinii\u2014 soluble antigen in circulation and immunosuppression in vitro","volume":"67","author":"Dasgupta","year":"1978","journal-title":"Indian Journal of Medical Research"},{"key":"S0022149X00012001_ref003","first-page":"345","volume-title":"Affinity chromatography\u2014Methods in Enzymology","volume":"22","author":"Cuatrecasas","year":"1971"},{"key":"S0022149X00012001_ref012","first-page":"567","article-title":"Excretory-secretory and somatic antigens in the diagnosis of human filariasis","volume":"56","author":"Kaushal","year":"1984","journal-title":"Clinical and Experimental Immunology"},{"key":"S0022149X00012001_ref015","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0021-9258(19)52451-6","article-title":"Protein measurement with the Folin-Phenol reagent","volume":"193","author":"Lowry","year":"1951","journal-title":"Journal of Biological Chemistry"},{"key":"S0022149X00012001_ref024","first-page":"427","article-title":"Studies on the skin test antigen FST for immunodiagnosis of filariasis. 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