{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T00:48:40Z","timestamp":1789778920698,"version":"4.0.1"},"reference-count":11,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T00:00:00Z","timestamp":1244160000000},"content-version":"unspecified","delay-in-days":6944,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[1990,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Immunoreactive components of<jats:italic>Opisthorchis viverrini<\/jats:italic>and<jats:italic>Clonorchis sinensis<\/jats:italic>were analysed by enzyme-linked immunosorbent assay (ELISA), radioimmunoprecipitation and immunoblotting. Somatic extracts from these two liver flukes as well as from other related parasites, together with the metabolic products, were tested for their reactivities with sera from patients with opisthorchiasis and clonorchiasis. A significant cross-reactivity in the ELISA was noted between<jats:italic>Opisthorchis<\/jats:italic>and<jats:italic>Clonorchis<\/jats:italic>. Immunoblotting and radioimmunoprecipitation analyses showed that the 89-kD protein which was previously shown to be a predominant metabolic product of<jats:italic>O. viverrini<\/jats:italic>reacted with sera from both groups of patients. However, an antigen with a molecular weight of 16 kD, apparently a predominant somatic component, appeared to be specific for<jats:italic>O. viverrini<\/jats:italic>.<\/jats:p>","DOI":"10.1017\/s0022149x00012049","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T15:15:42Z","timestamp":1244214942000},"page":"133-138","source":"Crossref","is-referenced-by-count":21,"title":["Immunological analysis of<i>Opisthorchis<\/i>and<i>Clonorchis<\/i>antigens"],"prefix":"10.1017","volume":"64","author":[{"given":"S.","family":"Sirisinha","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Sahassananda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Bunnag","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H. J.","family":"Rim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2009,6,5]]},"reference":[{"key":"S0022149X00012049_ref005","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(87)90154-4"},{"key":"S0022149X00012049_ref010","doi-asserted-by":"publisher","DOI":"10.2307\/3281001"},{"key":"S0022149X00012049_ref001","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3024.1990.tb00987.x"},{"key":"S0022149X00012049_ref002","doi-asserted-by":"crossref","first-page":"847","DOI":"10.1128\/JCM.23.5.847-851.1986","article-title":"Enzyme-linked immunosorbent assays for detection of antibodies to Gnathostoma antigen in patients with intermittent cutaneous migratory swellings","volume":"23","author":"Dharmkrong-At","year":"1986","journal-title":"Journal of Clinical Microbiology"},{"key":"S0022149X00012049_ref009","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.9.4350"},{"key":"S0022149X00012049_ref006","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0021-9258(19)52451-6","article-title":"Protein measurement with the Folin phenol reagent","volume":"193","author":"Lowry","year":"1951","journal-title":"Journal of Biological Chemistry"},{"key":"S0022149X00012049_ref003","doi-asserted-by":"publisher","DOI":"10.1042\/bj0890114"},{"key":"S0022149X00012049_ref004","unstructured":"Harinasuta C. (1969) Opisthorchiasis in Thailand: a review. In: Proceedings of Southeast Asian Seminar on Parasitology and Tropical Medicine, Schistosomiasis and other Snail-transmitted Helminthiasis (editor Harinasuta C. ). pp. 253\u2013264."},{"key":"S0022149X00012049_ref007","doi-asserted-by":"publisher","DOI":"10.3347\/kjp.1986.24.Suppl.1"},{"key":"S0022149X00012049_ref008","first-page":"208","article-title":"Pathology and immunology of Clonorchis sinensis infection of the liver","volume":"14","author":"Sun","year":"1984","journal-title":"Annals of Clinical and Laboratory Science"},{"key":"S0022149X00012049_ref011","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000081701"}],"container-title":["Journal of Helminthology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0022149X00012049","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,10,6]],"date-time":"2021-10-06T23:21:16Z","timestamp":1633562476000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0022149X00012049\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,6]]},"references-count":11,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1990,6]]}},"alternative-id":["S0022149X00012049"],"URL":"https:\/\/doi.org\/10.1017\/s0022149x00012049","relation":{},"ISSN":["0022-149X","1475-2697"],"issn-type":[{"value":"0022-149X","type":"print"},{"value":"1475-2697","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,6]]}}}