{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T16:10:29Z","timestamp":1791389429794,"version":"4.3.3"},"reference-count":20,"publisher":"Cambridge University Press (CUP)","issue":"4","license":[{"start":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T00:00:00Z","timestamp":1244160000000},"content-version":"unspecified","delay-in-days":6761,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[1990,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>To increase our understanding of the immune response to <jats:italic>Anisakis<\/jats:italic> infection, antigen specific IgG, IgA and IgE responses were identified using an immunoblot technique after polyacrylamide gel electrophoresis of excretory-secretory products from the larval stage of <jats:italic>Anisakis simplex<\/jats:italic>. Nine sera were drawn from proven cases of gastric anisakiasis within 3 days after symptoms had developed. The molecular weight of the major antigenic bands were distributed between 50 kDa and 120 kDa of the antigens. In nine cases of gastric anisakiasis, three of them were positive for IgG response, five for IgE, and six for IgA, respectively. None of control sera recognized the antigenic bands in IgA and IgE responses. In contrast, two controls had IgG antibodies against 1\u20132 proteins in the 65\u201395 kDa region. The antigenicity of the excretory\u2013secretory products was lost following treatment by 0\u00b72% trypsin, but not by 0\u00b72 M periodic acid. Based on the results of reactivity to lectins, antigenic bands of the ES products possessed mucin type glycoconjugate residues in their protein portion. This indicates that the humoral responses of IgA and IgE antibodies to the larval ES antigens are a more reliable index of infection than that of the IgG response.<\/jats:p>","DOI":"10.1017\/s0022149x00012359","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T15:15:42Z","timestamp":1244214942000},"page":"310-318","source":"Crossref","is-referenced-by-count":37,"title":["Immunoblot analysis of serum IgG, IgA and IgE responses against larval excretory-secretory antigens of <i>Anisakis simplex<\/i> in patients with gastric anisakiasis"],"prefix":"10.1017","volume":"64","author":[{"given":"Nobuaki","family":"Akao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taka-Aki","family":"Ohyama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaoru","family":"Kondo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2009,6,5]]},"reference":[{"key":"S0022149X00012359_ref019","first-page":"708","article-title":"Two cases of extra-gastrointestinal anisakiasis and evaluation of immunodiagnosis","volume":"28","author":"Yoshimura","year":"1980","journal-title":"Japanese Journal of Clinical Pathology"},{"key":"S0022149X00012359_ref015","doi-asserted-by":"publisher","DOI":"10.2307\/3281854"},{"key":"S0022149X00012359_ref013","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(86)90012-3"},{"key":"S0022149X00012359_ref018","first-page":"347","article-title":"Clinicopathological studies on larval anisakiasis, with special reference to the report of extra-gastrointestinal anisakiasis","volume":"28","author":"Yoshimura","year":"1979","journal-title":"Japanese Journal of Parasitology"},{"key":"S0022149X00012359_ref020","unstructured":"Yoshimura H. , Kondo K. , Ohnishi Y. & Akao N. (1983) Studies on immunodiagnosis of anisakiasis. Report for Scientific Research Grant from Ministry of Education of Japan."},{"key":"S0022149X00012359_ref014","first-page":"232","article-title":"Studies on the immunological diagnosis of anisakiasis: II. Isolation and purification of anisakis antigen","volume":"18","author":"Suzuki","year":"1969","journal-title":"Japanese Journal of Parasitology"},{"key":"S0022149X00012359_ref006","first-page":"31","article-title":"Gastric anisakiasis presenting acute gastrointestinal symptoms\u2014with special reference to the endoscopic and roentgenographic findings of Anisakis larva penetrating into the wall of the human stomach and to its clinical features","volume":"8","author":"Kawauchi","year":"1973","journal-title":"Stomach and Intestine"},{"key":"S0022149X00012359_ref010","first-page":"1437","article-title":"Diagnosis of acute gastric anisakiasis","volume":"5","author":"Namiki","year":"1970","journal-title":"Stomach and Intestine"},{"key":"S0022149X00012359_ref003","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(85)90351-7"},{"key":"S0022149X00012359_ref017","first-page":"213","article-title":"Establishment of a sero-diagnostic assay system for anisakiasis using a monoclonal antibody specific for Anisakis larval antigen","volume":"148","author":"Yagihashi","year":"1989","journal-title":"Journal of Clinical and Experimental Medicine"},{"key":"S0022149X00012359_ref002","doi-asserted-by":"publisher","DOI":"10.1007\/978-4-431-68290-5_14"},{"key":"S0022149X00012359_ref007","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(85)90031-4"},{"key":"S0022149X00012359_ref008","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"S0022149X00012359_ref001","first-page":"395","article-title":"Glycoconjugates of excretory-secretory antigens of second stage larvae of Toxocara canis: Analysis of their reactivity to lectins","volume":"35","author":"Akao","year":"1986","journal-title":"Japanese Journal of Parasitology"},{"key":"S0022149X00012359_ref009","first-page":"265","article-title":"Protein measurement with the Folin phenol reagent","volume":"193","author":"Lowry","year":"1951","journal-title":"Journal of Biological Chemistry"},{"key":"S0022149X00012359_ref004","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(83)90237-3"},{"key":"S0022149X00012359_ref011","doi-asserted-by":"publisher","DOI":"10.1007\/978-4-431-68290-5_18"},{"key":"S0022149X00012359_ref012","first-page":"37","volume-title":"Gastricanisakiasis in Japan, epidemiology, diagnosis, treatment","author":"Ohtaki","year":"1989"},{"key":"S0022149X00012359_ref016","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.9.4350"},{"key":"S0022149X00012359_ref005","first-page":"76","article-title":"List of the larvae of Anisakis spp. recorded from marine fish and squids caught off the Japan and its offshore islands","volume":"19","author":"Kagei","year":"1970","journal-title":"Bulletin of the Institute of Public Health"}],"container-title":["Journal of Helminthology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0022149X00012359","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,5,18]],"date-time":"2019-05-18T19:38:39Z","timestamp":1558208319000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0022149X00012359\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,12]]},"references-count":20,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1990,12]]}},"alternative-id":["S0022149X00012359"],"URL":"https:\/\/doi.org\/10.1017\/s0022149x00012359","relation":{},"ISSN":["0022-149X","1475-2697"],"issn-type":[{"value":"0022-149X","type":"print"},{"value":"1475-2697","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,12]]}}}