{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,3,31]],"date-time":"2022-03-31T18:15:07Z","timestamp":1648750507025},"reference-count":14,"publisher":"Cambridge University Press (CUP)","issue":"4","license":[{"start":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T00:00:00Z","timestamp":1244160000000},"content-version":"unspecified","delay-in-days":5665,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[1993,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Experimental completion of the life-cycle of <jats:italic>Paragonimus westermani<\/jats:italic> (Trematoda: Troglotrematidae) (diploid type) from egg to adult has been achieved in the laboratory for the first time. A dog was fed metacercariae of <jats:italic>P. westermani<\/jats:italic> from naturally infected freshwater crabs, <jats:italic>Geothelphusa dehaani<\/jats:italic>. The adult flukes were recovered and their eggs were incubated in water at 28\u00b0C, hatching after 20 days. Laboratory reared marsh snails, <jats:italic>Semisulcospira libertina<\/jats:italic>, were exposed to miracidia from embryonated eggs. One of 47 snails became infected with a large number of 2nd generation rediae containing fully mature cercariae 245 days after exposure. <jats:italic>Paragonimus<\/jats:italic>-free crabs (<jats:italic>G. dehaani<\/jats:italic>) were then infected with the intramolluscan larvae. Of the 40 crabs examined 34\u201370 days after infection, 17 were infected with a total of 84 metacercariae. A cat and 4 dogs were fed with 55 metacercariae obtained. Eighty to 100 days later, a total of 40 flukes was recovered; 39 flukes were adults with eggs in their Uteri.<\/jats:p>","DOI":"10.1017\/s0022149x00013237","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T15:10:18Z","timestamp":1244214618000},"page":"253-258","source":"Crossref","is-referenced-by-count":2,"title":["Diploid <i>Paragonimus westermani<\/i>: the experimental completion of its life-cycle"],"prefix":"10.1017","volume":"67","author":[{"given":"T.","family":"Shibahara","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2009,6,5]]},"reference":[{"key":"S0022149X00013237_ref009","first-page":"293","article-title":"Studies on the lung fluke, Paragonimus westermani (diploid type), in northern part of Hyogo Prefecture, Japan. II. Experimental oral infection with metacercariae to dogs and cats, with reference to morphological characteristics of adult worms and eggs","volume":"32","author":"Shibahara","year":"1983","journal-title":"Japanese Journal of Parasitology"},{"key":"S0022149X00013237_ref014","doi-asserted-by":"publisher","DOI":"10.7883\/yoken1952.6.107"},{"key":"S0022149X00013237_ref013","doi-asserted-by":"publisher","DOI":"10.1292\/jvms1939.51.315"},{"key":"S0022149X00013237_ref003","first-page":"1","article-title":"Investigations on the cercariae in Korea, I","volume":"21","author":"Kobayashi","year":"1918","journal-title":"Chosen Igakkai Zasshi"},{"key":"S0022149X00013237_ref001","first-page":"861","article-title":"An investigation of the first intermediate host of the lung fluke, especially on the development of encysted cercaria in the body of crabs 1st report","volume":"2175","author":"Ando","year":"1919","journal-title":"Tokyo Iji Shinshi"},{"key":"S0022149X00013237_ref010","first-page":"165","article-title":"Experimental infection of raccoon dogs with two types (diploid and triploid) of the lung fluke, Paragonimus westermani","volume":"34","author":"Shibahara","year":"1984","journal-title":"Japanese Journal of Parasitology"},{"key":"S0022149X00013237_ref011","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X00010415"},{"key":"S0022149X00013237_ref002","first-page":"493","article-title":"Experimental infection of Semisulcospira libertina with Paragonimus westermani (triploid type)","volume":"30","author":"Hamajima","year":"1981","journal-title":"Japanese Journal of Parasitology"},{"key":"S0022149X00013237_ref004","first-page":"251","volume-title":"Two types of the lung fluke which have been called Paragonimus westermani","volume":"5","author":"Miyazaki","year":"1978"},{"key":"S0022149X00013237_ref007","first-page":"23","article-title":"Chromosomes of a lung fluke, Paragonimus westermani","volume":"20","author":"Sakaguchi","year":"1976","journal-title":"Chromosome Information Service"},{"key":"S0022149X00013237_ref012","first-page":"173","article-title":"Experimental completion of the life cycle of the lung fluke, Paragonimxus westermani, in the laboratory","volume":"30","author":"Shimazu","year":"1981","journal-title":"Japanese Journal of Parasitology"},{"key":"S0022149X00013237_ref006","first-page":"349","article-title":"On the cercariae of Paragonimus westermani","volume":"2062","author":"Nakagawa","year":"1918","journal-title":"Tokyo Iji Shinshi"},{"key":"S0022149X00013237_ref008","first-page":"545","article-title":"Studies on the lung fluke, Paragonimus westermani (diploid type), in northern part of Hyogo Prefecture, Japan. I. Geographical distribution in Toyooka City and Izushi-gun, and morphological characteristics of metacercariae","volume":"31","author":"Shibahara","year":"1982","journal-title":"Japanese Journal of Parasitology"},{"key":"S0022149X00013237_ref005","first-page":"464","article-title":"A preliminary report on the discovery of the intermediate host of the human lung fluke","volume":"1910","author":"Nakagawa","year":"1915","journal-title":"Tokyo Iji Shinshi"}],"container-title":["Journal of Helminthology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0022149X00013237","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,6]],"date-time":"2019-04-06T19:28:43Z","timestamp":1554578923000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0022149X00013237\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,12]]},"references-count":14,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1993,12]]}},"alternative-id":["S0022149X00013237"],"URL":"https:\/\/doi.org\/10.1017\/s0022149x00013237","relation":{},"ISSN":["0022-149X","1475-2697"],"issn-type":[{"value":"0022-149X","type":"print"},{"value":"1475-2697","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,12]]}}}