{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,4,11]],"date-time":"2024-04-11T10:29:34Z","timestamp":1712831374722},"reference-count":11,"publisher":"Cambridge University Press (CUP)","issue":"1","license":[{"start":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T00:00:00Z","timestamp":1244160000000},"content-version":"unspecified","delay-in-days":5210,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[1995,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The outer cysts (Oc) of<jats:italic>Fasciola gigantica<\/jats:italic>metacercariae (Mc) were separated in an aqueous two-phase system composed of polyethylene glycol and dextran in distilled water. A large number of separated Mc without Oc were recovered in the bottom layer rich in dextran in the system. Ten separated Mc in the layer were used to inoculate the mouse with a syringe and juveniles were recovered from the animals on the 14th day. When the number of juveniles recovered was compared with that from the mice infected orally with the same number of Mc, there was a significant difference between them (P &lt;0.05). The former method is easier than the latter for inoculation.<\/jats:p>","DOI":"10.1017\/s0022149x00013882","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T15:06:29Z","timestamp":1244214389000},"page":"85-87","source":"Crossref","is-referenced-by-count":3,"title":["Separation of outer cysts from metacercariae of<i>Fasciola gigantica<\/i>in an aqueous two-phase system"],"prefix":"10.1017","volume":"69","author":[{"given":"S.","family":"Yoshihara","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Hazeyama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Kato","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"Goto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2009,6,5]]},"reference":[{"key":"S0022149X00013882_ref011","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(68)90214-0"},{"key":"S0022149X00013882_ref009","doi-asserted-by":"crossref","first-page":"498","DOI":"10.1016\/S0034-5288(18)34730-1","article-title":"Liver damage and the host-parasite relationship in experimental fascioliasis in the albino rat","volume":"6","author":"Thorpe","year":"1965","journal-title":"Research in Veterinary Science"},{"key":"S0022149X00013882_ref005","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/S0034-5288(18)32616-X","article-title":"The different stages of Fasciola hepatica capable of inducing immunity and the susceptibility of various stages to immunological attack in sensitised rat","volume":"30","author":"Hughes","year":"1981","journal-title":"Research in Veterinary Science"},{"key":"S0022149X00013882_ref002","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X00022343"},{"key":"S0022149X00013882_ref007","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1016\/S0034-5288(18)32267-7","article-title":"Protection against Fasciola hepatica in rats with adult fluke antigen in Freund's adjuvant: influence of antigen batch, antigen dose and number of sensitising injections","volume":"34","author":"Oldham","year":"1983","journal-title":"Research in Veterinary Science"},{"key":"S0022149X00013882_ref001","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-308X(08)60435-2"},{"key":"S0022149X00013882_ref008","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1128\/JB.82.3.331-341.1961","article-title":"Behavior of bacterial spores in aqueous polymer two-phase systems","volume":"82","author":"Sacks","year":"1961","journal-title":"Journal of Bacteriology"},{"key":"S0022149X00013882_ref004","doi-asserted-by":"crossref","first-page":"552","DOI":"10.1128\/JB.84.3.552-558.1962","article-title":"Procedure for cleaning of Clostridium botulinum spores","volume":"84","author":"Grecz","year":"1962","journal-title":"Journal of Bacteriology"},{"key":"S0022149X00013882_ref006","first-page":"186","article-title":"Response of lambs to challenge infections after repeated inoculation with Fasciola hepatica cysts","volume":"47","author":"Knight","year":"1980","journal-title":"Proceedings of the Helminthological Society of Washington"},{"key":"S0022149X00013882_ref010","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(64)90071-6"},{"key":"S0022149X00013882_ref003","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4017(85)90098-6"}],"container-title":["Journal of Helminthology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0022149X00013882","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,19]],"date-time":"2020-05-19T06:53:12Z","timestamp":1589871192000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0022149X00013882\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,3]]},"references-count":11,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1995,3]]}},"alternative-id":["S0022149X00013882"],"URL":"https:\/\/doi.org\/10.1017\/s0022149x00013882","relation":{},"ISSN":["0022-149X","1475-2697"],"issn-type":[{"value":"0022-149X","type":"print"},{"value":"1475-2697","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,3]]}}}