{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,30]],"date-time":"2025-11-30T22:26:55Z","timestamp":1764541615681,"version":"3.37.0"},"reference-count":22,"publisher":"Cambridge University Press (CUP)","issue":"4","license":[{"start":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T00:00:00Z","timestamp":1244160000000},"content-version":"unspecified","delay-in-days":6030,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[1992,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Infective first stage larvae of<jats:italic>Trichinella spiralis<\/jats:italic>were recovered from muscles of laboratory infected mice by digesting the muscles with 1% HC1\u20131% pepsin and collecting the larvae by modified Baerman's method. The larvae were cultivated in a serum-free medium for 18 h. The ES antigen obtained from the culture medium was used in an enzyme-linked immunosorbent assay (ELISA) for detecting IgG antibodies to<jats:italic>T. spiralis<\/jats:italic>in serum samples collected from three groups of individuals. The individuals of the first group were parasitologically confirmed trichinellosis patients, while those of group 2 were patients with other helminthiasis and group 3 were healthy, parasite-free individuals. The specificity of the assay was 100%. The sensitivity of the test was also 100% when performed on sera of group I collected at days 57 and 120 after infection. Sera collected earlier (day 23) and those collected 700 days after infection had negligible reactivity. Thus IgG-ELISA using ES antigen of the L<jats:sub>1<\/jats:sub>was useful not only for diagnosis but also in evaluation of cure. Western blot analysis revealed that specific antigens of<jats:italic>T. spiralis<\/jats:italic>were 94, 67, 63, and 39 kilodalton components.<\/jats:p>","DOI":"10.1017\/s0022149x00014759","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T15:12:24Z","timestamp":1244214744000},"page":"297-304","source":"Crossref","is-referenced-by-count":15,"title":["Immunodiagnosis of human trichinellosis using excretory-secretory (ES) antigen"],"prefix":"10.1017","volume":"66","author":[{"given":"Pakpimol","family":"Mahannop","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wanpen","family":"Chaicumpa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Prasert","family":"Setasuban","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nimit","family":"Morakote","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pramuan","family":"Tapchaisri","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2009,6,5]]},"reference":[{"key":"S0022149X00014759_ref020","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0450.1980.tb02031.x"},{"key":"S0022149X00014759_ref017","first-page":"73","article-title":"Trichinellosis in Thailand: epidemiology and biochemical identification of the aetiological agent","volume":"40","author":"Pozio","year":"1989","journal-title":"Tropical Medicine and Parasitology"},{"key":"S0022149X00014759_ref016","first-page":"1078","volume-title":"Manual of Clinical Immunology","author":"Palmer","year":"1980"},{"key":"S0022149X00014759_ref012","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"S0022149X00014759_ref009","first-page":"399","volume-title":"Trichinosis, Proceeding of the Fifth International Conference on Trichnellosis Survey","author":"Knapen","year":"1980"},{"key":"S0022149X00014759_ref014","doi-asserted-by":"crossref","first-page":"1046","DOI":"10.2460\/ajvr.1986.47.05.1046","article-title":"Field evaluation of enzyme-linked immunosorbent assay for swine trichinosis. 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