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Helminthol."],"published-print":{"date-parts":[[1995,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>To investigate antigenic differences between the developmental stages of<jats:italic>Anisakis simplex<\/jats:italic>, somatic, excretion\u2013secretion and detergent-solubilized surface antigen preparations of third- and fourth-stage<jats:italic>A. simplex<\/jats:italic>larvae, and a somatic antigen preparation of adults, were characterized by SDS\u2013PAGE and immunoblotting using mouse immune serum containing antibodies against the two larval stages. Excretion\u2013secretion and surface antigen preparations from thirdstage larvae behaved very differently from those of fourth-stage larvae, in both SDS-PAGE and immunoblotting; this suggests that certain excretion\u2013secretion and surface antigens are highly stage-specific. By contrast, somatic components of third- and fourth-stage larvae were found to have very similar banding patterns, suggesting conservation of these components during the development of the parasite in mice. The SDS-PAGE and immunoblotting results for adult somatic components seem to support this hypothesis<\/jats:p>","DOI":"10.1017\/s0022149x00014899","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T15:05:45Z","timestamp":1244214345000},"page":"319-324","source":"Crossref","is-referenced-by-count":11,"title":["<i>Anisakis simplex<\/i>: stage-specific antigens recognized by 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