{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,12]],"date-time":"2025-05-12T14:05:40Z","timestamp":1747058740029},"reference-count":12,"publisher":"Cambridge University Press (CUP)","issue":"4","license":[{"start":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T00:00:00Z","timestamp":1244160000000},"content-version":"unspecified","delay-in-days":4935,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[1995,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The assay system for antibody responses against Em2, the most specific antigen for serodiagnosis of alveolar echinococcosis (AE), has been established by enzyme-linked immunosorbent assay (ELISA) but not by Western blot assay, since Em2 antigen is not protein but carbohydrate in nature. Recently we reported that previously undescribed protein epitopes, designated Em18 and Em16 due to their molecular weights, were good serologic markers for AE by Western blot analysis. It has been shown that Em18 and Em16 are the only two epitopes recognized by commercially available weak positive (cut off) sera for the Em2<jats:sup>plus<\/jats:sup>-ELISA.<\/jats:p>","DOI":"10.1017\/s0022149x0001498x","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T15:05:45Z","timestamp":1244214345000},"page":"369-371","source":"Crossref","is-referenced-by-count":3,"title":["Em18 and Em16, new serologic marker epitopes for alveolar echinococcosis in Western blot analysis, are the only two epitopes recognized by commercially available weak positive (cut off) sera for Em2<sup>plus<\/sup>-ELISA"],"prefix":"10.1017","volume":"69","author":[{"given":"A.","family":"Ito","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.","family":"Osawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Nakao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Horii","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Okamoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Itoh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Yamashita","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2009,6,5]]},"reference":[{"key":"S0022149X0001498X_ref012","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1994.51.741"},{"key":"S0022149X0001498X_ref009","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1995.52.41"},{"key":"S0022149X0001498X_ref007","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(93)90476-7"},{"key":"S0022149X0001498X_ref002","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000079300"},{"key":"S0022149X0001498X_ref001","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(92)92693-A"},{"key":"S0022149X0001498X_ref004","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-308X(08)60024-X"},{"key":"S0022149X0001498X_ref003","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3024.1985.tb00070.x"},{"key":"S0022149X0001498X_ref011","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2125.1993.tb04182.x"},{"key":"S0022149X0001498X_ref005","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1128\/JCM.31.2.373-376.1993","article-title":"Improved primary immunodiagnosis of alveolar echinococcosis in humans by an enzyme-linked immunosorbent assay using the EM2plus antigen","volume":"31","author":"Gottstein","year":"1993","journal-title":"Journal of Clinical Microbiology"},{"key":"S0022149X0001498X_ref010","doi-asserted-by":"publisher","DOI":"10.1016\/0169-4758(91)90185-Q"},{"key":"S0022149X0001498X_ref008","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1993.49.208"},{"key":"S0022149X0001498X_ref006","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.1994.tb06549.x"}],"container-title":["Journal of Helminthology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0022149X0001498X","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,19]],"date-time":"2020-05-19T06:53:13Z","timestamp":1589871193000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0022149X0001498X\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,12]]},"references-count":12,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1995,12]]}},"alternative-id":["S0022149X0001498X"],"URL":"https:\/\/doi.org\/10.1017\/s0022149x0001498x","relation":{},"ISSN":["0022-149X","1475-2697"],"issn-type":[{"value":"0022-149X","type":"print"},{"value":"1475-2697","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,12]]}}}