{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,4,1]],"date-time":"2022-04-01T09:28:49Z","timestamp":1648805329879},"reference-count":21,"publisher":"Cambridge University Press (CUP)","issue":"1","license":[{"start":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T00:00:00Z","timestamp":1244160000000},"content-version":"unspecified","delay-in-days":4844,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[1996,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The activities of selected enzymes of the respiratory chain system in <jats:italic>Onchocerca fasciata<\/jats:italic> (Filarioidea: Onchocercidae) have been investigated histochemically. Thus, the localization and distributions of NADH dehydrogenase (EC 1.6.99.3), succinate dehydrogenase (SDH) (EC 1.3.99.1) and cytochrome oxidase (EC 1.9.3.1) were investigated in various tissues of the adult female worm by employing MTT, Nitro BT (dehydrogenases) and DAB (cytochrome oxidase). Different tissues varied considerably in their enzymatic activities. The hypodermis and reproductive tissues showed strong and identical localization of NADH and SDH dehydrogenase activities reflecting high metabolic rates. Little or no dehydrogenase activities were observed in the intestine and cuticle. In contrast to the two dehyrogenases, no activity was observed for cytochrome oxidase in any of the tissues in adult or embryonic stages of the worm. The significance of these results with respect to the energy metabolism of the worm is discussed. It is suggested that <jats:italic>O. fasciata<\/jats:italic> lacks a classical, mammalian-type respiratory pathway and that oxidative phosphorylation is of no importance as an energy generating pathway in this essentially anaerobic parasite.<\/jats:p>","DOI":"10.1017\/s0022149x00015121","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T15:04:41Z","timestamp":1244214281000},"page":"47-51","source":"Crossref","is-referenced-by-count":1,"title":["<i>Onchocerca fasciata<\/i>: histochemical demonstration of succinate and NADH dehydrogenase"],"prefix":"10.1017","volume":"70","author":[{"given":"M.S.","family":"Omar","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.M.S.","family":"Raoof","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2009,6,5]]},"reference":[{"key":"S0022149X00015121_ref019","first-page":"418","article-title":"Vascular perfusion of Onchocerca volvulus nodules","volume":"39","author":"Smith","year":"1988","journal-title":"Tropical Medicine and Parasitology"},{"key":"S0022149X00015121_ref016","doi-asserted-by":"publisher","DOI":"10.1016\/0020-7519(89)90145-8"},{"key":"S0022149X00015121_ref012","doi-asserted-by":"publisher","DOI":"10.1007\/BF00932677"},{"key":"S0022149X00015121_ref017","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(85)90025-8"},{"key":"S0022149X00015121_ref009","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(85)90061-1"},{"key":"S0022149X00015121_ref021","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(87)90067-1"},{"key":"S0022149X00015121_ref015","article-title":"Onchocerca fasciata: Enzyme histochemistry and tissue distribution of various dehydrogenases in adult female worm","volume":"81","author":"Omar","year":"1995","journal-title":"Parasitology Research"},{"key":"S0022149X00015121_ref018","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.38.1.1"},{"key":"S0022149X00015121_ref003","doi-asserted-by":"publisher","DOI":"10.1016\/0020-7519(86)90081-0"},{"key":"S0022149X00015121_ref014","first-page":"295","article-title":"Histochemical distribution of esterases in adult Onchocerca fasciata (Filarioidea: Onchocercidae)","volume":"44","author":"Omar","year":"1993","journal-title":"Tropical Medicine and Parasitology"},{"key":"S0022149X00015121_ref013","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X00001607"},{"key":"S0022149X00015121_ref020","first-page":"537","volume-title":"Histochemistry","volume":"3","author":"Stoward","year":"1991"},{"key":"S0022149X00015121_ref005","doi-asserted-by":"publisher","DOI":"10.1016\/0020-7519(88)90031-8"},{"key":"S0022149X00015121_ref008","first-page":"15","article-title":"Energy generation in filariae - a target for chemotherapy","volume":"36","author":"Mendis","year":"1985","journal-title":"Tropical Medicine and Parasitology"},{"key":"S0022149X00015121_ref001","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-349-86119-4"},{"key":"S0022149X00015121_ref011","doi-asserted-by":"publisher","DOI":"10.1177\/5.4.420"},{"key":"S0022149X00015121_ref010","first-page":"26","article-title":"Characterization of respiratory electron transport in adult Onchocerca gutturosa","volume":"36","author":"Mendis","year":"1985","journal-title":"Tropical Medicine and Parasitology"},{"key":"S0022149X00015121_ref002","first-page":"1","article-title":"Biochemistry of filarial worms","volume":"52","author":"Barrett","year":"1983","journal-title":"Helminthological Abstracts Series A"},{"key":"S0022149X00015121_ref004","doi-asserted-by":"publisher","DOI":"10.1016\/0169-4758(86)90116-X"},{"key":"S0022149X00015121_ref007","first-page":"359","article-title":"The morphology of adult Onchocerca vohulus based on electron microscopy","volume":"39","author":"Franz","year":"1988","journal-title":"Tropical Medicine and Parasitology"},{"key":"S0022149X00015121_ref006","first-page":"459","volume-title":"Histochemistry in pathology","author":"Filipe","year":"1990"}],"container-title":["Journal of Helminthology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0022149X00015121","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,5,12]],"date-time":"2019-05-12T22:12:33Z","timestamp":1557699153000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0022149X00015121\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,3]]},"references-count":21,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1996,3]]}},"alternative-id":["S0022149X00015121"],"URL":"https:\/\/doi.org\/10.1017\/s0022149x00015121","relation":{},"ISSN":["0022-149X","1475-2697"],"issn-type":[{"value":"0022-149X","type":"print"},{"value":"1475-2697","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,3]]}}}