{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,29]],"date-time":"2026-03-29T11:13:38Z","timestamp":1774782818344,"version":"3.50.1"},"reference-count":31,"publisher":"Cambridge University Press (CUP)","issue":"4","license":[{"start":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T00:00:00Z","timestamp":1244160000000},"content-version":"unspecified","delay-in-days":4569,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[1996,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The random amplified polymorphic DNA (RAPD) assay was used to find molecular markers able to distinguish <jats:italic>Trichinella spiralis<\/jats:italic> from <jats:italic>T. britovi,<\/jats:italic> the two recognized Spanish <jats:italic>Trichinella<\/jats:italic> species. Fourteen Spanish <jats:italic>Trichinella<\/jats:italic> isolates, as well as reference <jats:italic>Trichinella<\/jats:italic> isolates representing the five species <jats:italic>T. spiralis<\/jats:italic> (T1), <jats:italic>T. nativa<\/jats:italic> (T2), <jats:italic>T. britovi<\/jats:italic> (T3), <jats:italic>T. pseudospiralis<\/jats:italic> (T4) and <jats:italic>T. nelsoni<\/jats:italic> (T7) and the three other taxa <jats:italic>Trichinella<\/jats:italic> T5, <jats:italic>Trichinella<\/jats:italic> T6 and <jats:italic>Trichinella<\/jats:italic> T8 of the genus, were characterized by RAPD using both purified and crude DNAs from infective muscle larvae (ML) and seven arbitrary primers. Three primers yielded diagnostic RAPD markers for the Spanish <jats:italic>T. spiralis<\/jats:italic> and <jats:italic>T. britovi<\/jats:italic> isolates as well as for the <jats:italic>Trichinella<\/jats:italic> reference isolates analysed, and in the case of crude DNAs the results were obtained in few hours. In addition, the species-specificity of the diagnostic RAPD markers from Spanish <jats:italic>Trichinella<\/jats:italic> isolates was studied by cross-hybridization assays. These assays confirmed that the selected diagnostic DNA fragments were not species-specific, but showed potential differences in the copy number among the examined <jats:italic>Trichinella<\/jats:italic> genetic clusters.<\/jats:p>","DOI":"10.1017\/s0022149x00015637","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T11:01:52Z","timestamp":1244199712000},"page":"335-343","source":"Crossref","is-referenced-by-count":12,"title":["Characterization of Spanish <i>Trichinella<\/i> isolates by random amplified polymorphic DNA 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