{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,4,1]],"date-time":"2022-04-01T04:16:22Z","timestamp":1648786582285},"reference-count":12,"publisher":"Cambridge University Press (CUP)","issue":"4","license":[{"start":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T00:00:00Z","timestamp":1244160000000},"content-version":"unspecified","delay-in-days":4204,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[1997,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A comparative study was undertaken between an enzymatic immunohistochemical technique (EIT) developed for the diagnosis of human trichinellosis and the indirect immunofluorescence test (IIF), analysing sera from outbreaks of human trichinellosis in Argentina. The HIT was developed using a biotinylated anti-human gammaglobulin and a preformed macromolecular complex of avidin and biotinylated peroxidase. In both tests, the antigen used consisted of infected rat tissue sections containing muscle larval cysts of <jats:italic>Trichinella spiralis<\/jats:italic>. Results showed that the HIT closely correlated with IIP and also allowed diagnosis at an early stage of infection, thus helping to provide effective treatment for the disease. When the test was performed on sera from healthy individuals and those with other parasitic infections, cross-reactions were observed only with sera from patients with toxocariasis (1\/8), Chagas' disease (3\/17) and four out of 100 healthy individuals. No cross-reactions were observed with sera from patients with toxoplasmosis (0\/7) or hydatidosis (0\/8). Assay sensitivity was 100% and its specificity 93%. Since it renders no false negative results, BIT is an effective screening tool for detecting infection and should prove to be an important diagnostic technique for trichinellosis in rural areas and for epidemiological surveys.<\/jats:p>","DOI":"10.1017\/s0022149x00016102","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T14:59:33Z","timestamp":1244213973000},"page":"299-304","source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of an enzymatic immunohistochemical technique in human trichinellosis"],"prefix":"10.1017","volume":"71","author":[{"given":"G.J.M.","family":"Ben","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.L.","family":"Malmassari","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.G.","family":"Nu\u00f1ez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.N.","family":"Costantino","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.M.","family":"Venturiello","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2009,6,5]]},"reference":[{"key":"S0022149X00016102_ref005","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4017(83)90051-1"},{"key":"S0022149X00016102_ref001","first-page":"355","article-title":"Immunofluorescence in the serological diagnosis of Trichinella spiralis infection","volume":"17","author":"Brzosko","year":"1965","journal-title":"Experimental Medicine and Microbiology"},{"key":"S0022149X00016102_ref009","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4613-3578-8_12"},{"key":"S0022149X00016102_ref007","first-page":"97","volume-title":"Diccionario de epidemiologia","author":"Last","year":"1989"},{"key":"S0022149X00016102_ref006","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1093\/infdis\/105.2.97","article-title":"Fluorescent antibody studies of Trichinella spiralis infections","volume":"105","author":"Jackson","year":"1959","journal-title":"Journal of Infection and Disease"},{"key":"S0022149X00016102_ref002","first-page":"951","article-title":"Recognition of Trichinella spiralis muscle larvae antigens by sera from humans infected with this parasite and its potential use in diagnosis","volume":"8","author":"Chapa-Ruiz","year":"1990","journal-title":"Bulletin de la Societ\u00e9 Fran\u00e7aise de Parasitologie"},{"key":"S0022149X00016102_ref003","first-page":"599","article-title":"Evaluaci\u00f3n de la inmunofluorescencia indirecta en el diagn\u00f3stico y la seroepidemiolog\u00eda de la triquinosis humana","volume":"15","author":"Coltorti","year":"1981","journal-title":"Acta Bioqu\u00edmica Clinica Latinoamericana"},{"key":"S0022149X00016102_ref004","first-page":"221","article-title":"Kinetics of humoral response during the acute and the convalescent phase of human trichinosis","volume":"264","author":"Feldmeier","year":"1986","journal-title":"Zentralblatt f\u00fcr Bakteriologie und Hygiene"},{"key":"S0022149X00016102_ref008","first-page":"449","volume-title":"Trichinellosis","author":"Ljungstr\u00f6m","year":"1974"},{"key":"S0022149X00016102_ref011","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1975.tb29179.x"},{"key":"S0022149X00016102_ref012","first-page":"39","volume-title":"Epidemiologie appliqu\u00e9e a la lutte collective centre les maladies animales transmissibles majeures","author":"Toma","year":"1995"},{"key":"S0022149X00016102_ref010","first-page":"597","article-title":"Resolution N\u00b0 XI, Internal Parasitic Diseases with Reference to Immunological Response","volume":"9","year":"1990","journal-title":"Scientific and Technical Review. Office International des Epizootics"}],"container-title":["Journal of Helminthology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0022149X00016102","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,5,11]],"date-time":"2019-05-11T19:49:53Z","timestamp":1557604193000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0022149X00016102\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,12]]},"references-count":12,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1997,12]]}},"alternative-id":["S0022149X00016102"],"URL":"https:\/\/doi.org\/10.1017\/s0022149x00016102","relation":{},"ISSN":["0022-149X","1475-2697"],"issn-type":[{"value":"0022-149X","type":"print"},{"value":"1475-2697","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,12]]}}}