{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,9]],"date-time":"2025-04-09T05:42:17Z","timestamp":1744177337944},"reference-count":29,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T00:00:00Z","timestamp":1244160000000},"content-version":"unspecified","delay-in-days":4022,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[1998,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Incorporation of radiolabelled tyrosine was examined in males of<jats:italic>Schistosoma mansoni<\/jats:italic>maintained<jats:italic>in vitro<\/jats:italic>. Adult worms were recovered from mice and only those that were paired were used. Males and females were separated and placed in 4\u00b0C RPMI 1640 containing 10% fetal calf serum (FCS). Individual males were incubated for 1 h either alone or with a female. Other males were incubated in a medium containing 40 ng ml<jats:sup>\u22121<\/jats:sup>recombinant tumour necrosis factor \u03b1 (rTNF\u03b1). Subsequently, males were incubated with or without females for 1 h in a medium containing 20<jats:italic>\u03bc<\/jats:italic>Ci ml<jats:sup>\u22121<\/jats:sup>[<jats:sup>14<\/jats:sup>C]-tyrosine. All incubations were carried out in RPMI 1640 containing 10% FCS at 37\u00b0C under 5% CO<jats:sub>2<\/jats:sub>. Untreated single males incorporated small amounts of labelled tyrosine. Pretreatment of single males with rTNF\u03b1 did not affect their tyrosine incorporation (<jats:italic>P<\/jats:italic>= 0.693). However, males maintained<jats:italic>in vitro<\/jats:italic>with females incorporated 221% more tyrosine than the controls (<jats:italic>P<\/jats:italic>= 0.00008), suggesting that a chemical stimulus from the female affects tyrosine incorporation in males.<\/jats:p>","DOI":"10.1017\/s0022149x00016291","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T14:56:38Z","timestamp":1244213798000},"page":"123-126","source":"Crossref","is-referenced-by-count":9,"title":["<i>Schistosoma mansoni:<\/i>females enhance [<sup>14<\/sup>C]-tyrosine incorporation in males maintained<i>in 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