{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,4,12]],"date-time":"2024-04-12T03:09:46Z","timestamp":1712891386835},"reference-count":26,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T00:00:00Z","timestamp":1244160000000},"content-version":"unspecified","delay-in-days":4022,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[1998,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Adult<jats:italic>Strongylus vulgaris<\/jats:italic>, collected from the caecum of infected horses and embedded in paraplast using standard methods, were sectioned for immunohistochemistry (IHC) studies. Antibodies were raised in rabbit against the excretory\u2013secretory product (ESP) and against two constituent protein bands (28\u201330 kDa). The use of sodium dodecyl sulphate\u2013polyacrylamide gel electrophoresis (SDS-PAGE), enzyme-linked immunosorbent assay (ELISA) and immunoblotting indicated the immunogenicity of ESP and of the subunits (28\u201330 kDa). In ELISA, both rabbit hyperimmune sera recognized the ESP and (28\u201330 kDa) bands consistently and strongly. Both hyperimmune sera recognized most ESP subunits (80, 60, 54, 42, 35, 30, 20 and 15 kDa) in immunoblots. IHC, using light microscopy, suggested that the following worm tissues reacted strongly and positively with both antisera: amphids, tooth core, intestine, excretory gland and ducts, and hypodermis. Thus, either these are antigen-producing tissues, or antigens sharing common epitopes occur in them. The following tissues reacted weakly: body cuticle, buccal capsule cuticle, oesophagus, and also somatic muscle (non-contractile portion) perhaps due to diffusion of antigen from adjacent tissues. Preimmune serum gave a negative reaction with most worm tissues.<\/jats:p>","DOI":"10.1017\/s0022149x00016357","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T14:56:38Z","timestamp":1244213798000},"page":"159-166","source":"Crossref","is-referenced-by-count":6,"title":["An immunohistochemical investigation of the adult stage of the equine parasite<i>Strongylus vulgaris<\/i>"],"prefix":"10.1017","volume":"72","author":[{"given":"M.S.","family":"Mobarak","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.F.","family":"Ryan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2009,6,5]]},"reference":[{"key":"S0022149X00016357_ref025","volume-title":"Host influence on parasite physiology","author":"Weinstein","year":"1959"},{"key":"S0022149X00016357_ref024","doi-asserted-by":"publisher","DOI":"10.2307\/3282455"},{"key":"S0022149X00016357_ref022","first-page":"290","volume-title":"The development of strongyles in horses and the diseases","volume":"2","author":"Round","year":"1970"},{"key":"S0022149X00016357_ref020","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(88)90012-6"},{"key":"S0022149X00016357_ref018","first-page":"19","article-title":"Increase of immunoglobulin T concentration in ponies as a response to experimental infection with the nematode Strongylus vulgaris","volume":"39","author":"Patton","year":"1978","journal-title":"American Journal of Veterinary Research"},{"key":"S0022149X00016357_ref016","volume-title":"Strongylus vulgaris in the horse: its biology and veterinary importance","author":"Ogbourne","year":"1985"},{"key":"S0022149X00016357_ref015","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(87)90015-6"},{"key":"S0022149X00016357_ref013","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3024.1984.tb00779.x"},{"key":"S0022149X00016357_ref012","doi-asserted-by":"crossref","first-page":"654","DOI":"10.2460\/ajvr.1990.51.04.654","article-title":"Effects of repeated Strongylus vulgaris inoculations and concurrent ivermectin treatment on mesenteric arterial lesions in pony foals","volume":"4","author":"Klei","year":"1990","journal-title":"American Journal of Veterinary Research"},{"key":"S0022149X00016357_ref008","volume-title":"Antibodies. A laboratory manual","author":"Harlow","year":"1988"},{"key":"S0022149X00016357_ref006","doi-asserted-by":"crossref","first-page":"129","DOI":"10.4049\/jimmunol.109.1.129","article-title":"Enzyme-linked immunosorbent assay, ELISA","volume":"109","author":"Engvall","year":"1972","journal-title":"Journal of Immunology"},{"key":"S0022149X00016357_ref003","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4017(94)90120-1"},{"key":"S0022149X00016357_ref002","first-page":"1181","article-title":"Host response to experimentally induced infections of Strongylus vulgaris in parasite-free and naturally infected ponies","volume":"35","author":"Amborski","year":"1974","journal-title":"American journal of Veterinary Research"},{"key":"S0022149X00016357_ref021","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3024.1990.tb00953.x"},{"key":"S0022149X00016357_ref005","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-3306.1975.tb03267.x"},{"key":"S0022149X00016357_ref001","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-308X(08)60106-2"},{"key":"S0022149X00016357_ref009","doi-asserted-by":"publisher","DOI":"10.1094\/Phyto-80-709"},{"key":"S0022149X00016357_ref026","first-page":"259","article-title":"Antigenic analysis of tissues and excretory secretory products from Strongylus vulgaris","volume":"45","author":"Wynne","year":"1981","journal-title":"Canadian Journal of Comparative Medicine"},{"key":"S0022149X00016357_ref011","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4017(83)90007-9"},{"key":"S0022149X00016357_ref007","unstructured":"Ganghish K.S. (1994) Investigation of aminopeptidase and acetylcholinesterase activity derived from the adult stage of the equine parasitic nematodes Strongylus vulgaris and Strongylus edentatus (Looss, 1900) and of proteinase activity of adult Strongylus edentatus. MSc thesis, University College Dublin."},{"key":"S0022149X00016357_ref017","article-title":"Immunodiagnosis of Fasciola hepatica infection (fasciolosis) in a human population in the Bolivian Altiplano using purified cathepsin L cysteine proteinase","author":"O'Neill","journal-title":"American Journal of Tropical Medicine and Hygiene"},{"key":"S0022149X00016357_ref019","unstructured":"Place P. (1994) Purification of a secreted\u2013excreted proteinase by affinity chromatography. BSc thesis. University College Dublin."},{"key":"S0022149X00016357_ref004","first-page":"271","volume-title":"World animal science, parasites, pests and predators","author":"Drudge","year":"1985"},{"key":"S0022149X00016357_ref014","doi-asserted-by":"publisher","DOI":"10.1017\/S003118200005349X"},{"key":"S0022149X00016357_ref010","doi-asserted-by":"publisher","DOI":"10.1016\/0169-4758(86)90164-X"},{"key":"S0022149X00016357_ref023","doi-asserted-by":"crossref","first-page":"898","DOI":"10.4049\/jimmunol.132.2.898","article-title":"Antigens from Trichinella spiralis that induce a protective response in the mouse","volume":"132","author":"Silbertstein","year":"1984","journal-title":"Journal of Immunology"}],"container-title":["Journal of Helminthology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0022149X00016357","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,3,14]],"date-time":"2024-03-14T09:06:26Z","timestamp":1710407186000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0022149X00016357\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,6]]},"references-count":26,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1998,6]]}},"alternative-id":["S0022149X00016357"],"URL":"https:\/\/doi.org\/10.1017\/s0022149x00016357","relation":{},"ISSN":["0022-149X","1475-2697"],"issn-type":[{"value":"0022-149X","type":"print"},{"value":"1475-2697","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,6]]}}}