{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T11:29:12Z","timestamp":1779103752259,"version":"3.51.4"},"reference-count":18,"publisher":"Cambridge University Press (CUP)","issue":"3","license":[{"start":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T00:00:00Z","timestamp":1244160000000},"content-version":"unspecified","delay-in-days":3930,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[1998,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>IgG and IgM antibody responses to fluke cysteine proteinases in <jats:italic>Paragonimus ohirai<\/jats:italic>- and <jats:italic>Fasciola<\/jats:italic> sp.-infected rats were followed by means of cystatin capture ELISA using fluke excretory-secretory products for 10 weeks after infection. The specific IgG antibodies were detectable at week 2 postinfection in all <jats:italic>P. ohirai<\/jats:italic>-infected and some <jats:italic>Fasciola<\/jats:italic>-infected rats. Levels of specific IgG antibodies increased rapidly between week 2 and 6, and slightly thereafter, in both infected groups. From week 3, specific IgG antibody levels were higher in <jats:italic>Fasciola<\/jats:italic>-infected than <jats:italic>P. ohirai<\/jats:italic>-infected rats. Sera from infected rats did not react with heterologous cysteine proteinases throughout the infection periods. In both infected groups, the kinetic patterns of specific IgM antibody responses were similar to those of specific IgG antibody responses although the ELISA levels of the IgM antibody responses were much lower. In abnormal infections with <jats:italic>P. ohirai<\/jats:italic> metacercariae x-irradiated at 2 krad, the specific IgG antibodies were detectable at week 2 postinfection with similar ELISA values to normal <jats:italic>P. ohirai<\/jats:italic> infection, but thereafter increased little. In infections with <jats:italic>P. westermani<\/jats:italic>, for which the rat is not a suitable host, even stunted worms induced a comparable specific IgG antibody response, although the response was lower than in normal infections with <jats:italic>P. ohirai<\/jats:italic>. These results indicate that cystatin capture ELISA can distinguish clearly between <jats:italic>Paragonimus<\/jats:italic> and <jats:italic>Fasciola<\/jats:italic> infections which show immunodiagnostic cross-reactivity and is useful even in the early stages of the infection and in the infection of unsuitable hosts.<\/jats:p>","DOI":"10.1017\/s0022149x00016424","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T10:53:03Z","timestamp":1244199183000},"page":"187-191","source":"Crossref","is-referenced-by-count":10,"title":["Antibody responses to fluke cysteine proteinases in <i>Paragonimus<\/i>- and <i>Fasciola<\/i>-infected rats"],"prefix":"10.1017","volume":"72","author":[{"given":"T.","family":"Ikeda","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2009,6,5]]},"reference":[{"key":"S0022149X00016424_ref015","first-page":"144","article-title":"Human paragonimiasis in Japan","volume":"5","author":"Norimatu","year":"1986","journal-title":"Respiration Research"},{"key":"S0022149X00016424_ref017","first-page":"373","article-title":"A cysteine proteinase from the liver fluke Fasciola spp.: purification, characterization, localization and application to immunodiagnosis","volume":"38","author":"Yamasaki","year":"1989","journal-title":"Japanese Journal of Parasitology"},{"key":"S0022149X00016424_ref008","doi-asserted-by":"publisher","DOI":"10.1159\/000234505"},{"key":"S0022149X00016424_ref003","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1988.39.66"},{"key":"S0022149X00016424_ref002","first-page":"449","volume-title":"Clinical parasitology","author":"Beaver","year":"1984"},{"key":"S0022149X00016424_ref018","doi-asserted-by":"publisher","DOI":"10.2307\/3274900"},{"key":"S0022149X00016424_ref012","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1980.29.602"},{"key":"S0022149X00016424_ref011","doi-asserted-by":"publisher","DOI":"10.3347\/kjp.1986.24.2.187"},{"key":"S0022149X00016424_ref009","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1996.55.434"},{"key":"S0022149X00016424_ref016","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/S0034-5288(18)31925-8","article-title":"Partial purification of somatic and excretory-secretory products of adult Fasciola hepatica and their application for the serodiagnosis of experimental and natural fascioliasis using an ELISA","volume":"37","author":"Pfister","year":"1984","journal-title":"Research in Veterinary Science"},{"key":"S0022149X00016424_ref007","first-page":"377","article-title":"The effect of x-irradiation of metacercariae on infection of rats with Paragonimus ohirai","volume":"33","author":"Ikeda","year":"1984","journal-title":"Japanese Journal of Parasitology"},{"key":"S0022149X00016424_ref006","doi-asserted-by":"publisher","DOI":"10.2307\/3280804"},{"key":"S0022149X00016424_ref001","first-page":"963","article-title":"Supplement of the experimental infection with lung fluke to the small animals","volume":"1054","author":"Ando","year":"1920","journal-title":"Iji Shinbun"},{"key":"S0022149X00016424_ref010","first-page":"368","article-title":"Early clinical symptoms and signs of paragonimiasis westermani, time course when intradermal test with Paragonimus westermani antigen turns positive and effects of praziquantel to paragonimiasis","volume":"23","author":"Kanzaki","year":"1985","journal-title":"Japanese Journal of Thoracic Diseases"},{"key":"S0022149X00016424_ref004","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1990.42.335"},{"key":"S0022149X00016424_ref013","doi-asserted-by":"publisher","DOI":"10.1042\/bj2560433"},{"key":"S0022149X00016424_ref014","first-page":"17","article-title":"Studies on the lung fluke. (XII) Comparison of the development of Paragonimus ohirai Miyazaki, 1939 and Paragonimus westermani in the rats","volume":"2","author":"Miyazaki","year":"1946","journal-title":"Report of Kagoshima Medical College"},{"key":"S0022149X00016424_ref005","doi-asserted-by":"publisher","DOI":"10.2307\/3280344"}],"container-title":["Journal of Helminthology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0022149X00016424","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,5,10]],"date-time":"2019-05-10T16:20:01Z","timestamp":1557505201000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0022149X00016424\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,9]]},"references-count":18,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1998,9]]}},"alternative-id":["S0022149X00016424"],"URL":"https:\/\/doi.org\/10.1017\/s0022149x00016424","relation":{},"ISSN":["0022-149X","1475-2697"],"issn-type":[{"value":"0022-149X","type":"print"},{"value":"1475-2697","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,9]]}}}