{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T13:48:33Z","timestamp":1782913713202,"version":"3.54.5"},"reference-count":18,"publisher":"Cambridge University Press (CUP)","issue":"3","license":[{"start":{"date-parts":[[2007,9,1]],"date-time":"2007-09-01T00:00:00Z","timestamp":1188604800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[2007,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Using Western blot techniques, the specificities of crude and purified (PAK and PAS) <jats:italic>Anisakis simplex<\/jats:italic> antigens were compared against 24 sera from patients diagnosed with <jats:italic>Anisakis<\/jats:italic> sensitization. All patients recognized a 60\u00a0kDa protein against the <jats:italic>A<\/jats:italic>. <jats:italic>simplex<\/jats:italic> crude extract, while 37.5% and 12.5% reacted with proteins of 40 and 25\u00a0kDa, respectively, when IgG was tested. In the case of IgE determination, 41.6% of sera were negative, while 12.5% and 20.8% appeared to cross-react against <jats:italic>Toxocara canis<\/jats:italic> and <jats:italic>Ascaris suum<\/jats:italic>, respectively. When the PAK antigen (<jats:italic>A<\/jats:italic>. <jats:italic>simplex<\/jats:italic> antigen purified by means of a column of IgG anti-<jats:italic>A<\/jats:italic>. <jats:italic>simplex<\/jats:italic>) was tested, immune recognition towards the 60, 40 and 25\u00a0kDa proteins increased in 83.3%, 16.7% and 4.2%, respectively, when the Ig antibodies were tested. In the case of the PAS antigen (PAK antigen purified by means of a column of IgG anti-<jats:italic>A<\/jats:italic>. <jats:italic>suum<\/jats:italic>), the reaction against the 40 and 25\u00a0kDa proteins increased to 45.8% and 25%, respectively, when Ig antibodies were used. Finally, when the EAS antigen (eluted from the anti-<jats:italic>A<\/jats:italic>. <jats:italic>suum<\/jats:italic> column after PAK purification) was tested, 83.3% of the assayed sera reacted against the 14\u00a0kDa protein, when the Ig antibodies, IgG and IgM immunoglobulins were measured. With the IgE determination, the reactions were observed in 41.7% of patients with proteins between 60 and 35\u00a0kDa against the PAS antigen. With the EAS antigen, reactive bands of 184, 84 and 14\u00a0kDa appeared. In conclusion, in the purification process of the <jats:italic>A<\/jats:italic>. <jats:italic>simplex<\/jats:italic> larval crude extract, the proteins implicated in cross-reactions with <jats:italic>Ascaris<\/jats:italic> and <jats:italic>Toxocara<\/jats:italic> were eliminated, with an important concentration of proteins responsible for the induction of specific responses.<\/jats:p>","DOI":"10.1017\/s0022149x07820220","type":"journal-article","created":{"date-parts":[[2007,9,17]],"date-time":"2007-09-17T14:24:30Z","timestamp":1190039070000},"page":"307-310","source":"Crossref","is-referenced-by-count":7,"title":["Western blot antibody determination in sera from patients diagnosed with <i>Anisakis<\/i> sensitization with different antigenic fractions of <i>Anisakis simplex<\/i> purified by affinity chromatography"],"prefix":"10.1017","volume":"81","author":[{"given":"M.","family":"Rodero","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Cu\u00e9llar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Chivato","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.M.","family":"Mateos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Laguna","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2007,9,1]]},"reference":[{"key":"S0022149X07820220_ref20","doi-asserted-by":"publisher","DOI":"10.1016\/0020-7519(83)90008-5"},{"key":"S0022149X07820220_ref19","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.2.3.278"},{"key":"S0022149X07820220_ref16","doi-asserted-by":"crossref","first-page":"736","DOI":"10.1007\/s004360100439","article-title":"Purification of Anisakis simplex antigen by affinity chromatography","volume":"87","author":"Rodero","year":"2001","journal-title":"Parasitology Research"},{"key":"S0022149X07820220_ref12","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2000.106732"},{"key":"S0022149X07820220_ref8","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4612-2732-8_3"},{"key":"S0022149X07820220_ref5","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2005.11.007"},{"key":"S0022149X07820220_ref4","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X0001230X"},{"key":"S0022149X07820220_ref1","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X00010014"},{"key":"S0022149X07820220_ref3","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-6851(00)00192-4"},{"key":"S0022149X07820220_ref6","first-page":"21","volume-title":"Gel electrophoresis in proteins","author":"Hames","year":"1986"},{"key":"S0022149X07820220_ref14","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0450.1998.tb00833.x"},{"key":"S0022149X07820220_ref11","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2222.2000.00758.x"},{"key":"S0022149X07820220_ref9","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"S0022149X07820220_ref17","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02762002000200018"},{"key":"S0022149X07820220_ref18","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02762005000300015"},{"key":"S0022149X07820220_ref7","doi-asserted-by":"publisher","DOI":"10.1007\/s004360050335"},{"key":"S0022149X07820220_ref13","first-page":"762","article-title":"Analysis of antigens defined by anti-Anisakis larvae antibodies of IgE and IgG type in the sera of patients with acute gastrointestinal anisakiasis","volume":"87","author":"Nakata","year":"1990","journal-title":"Nippon Shokakibyo Gakkai Zasshi"},{"key":"S0022149X07820220_ref10","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2222.1999.00574.x"}],"container-title":["Journal of Helminthology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0022149X07820220","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,5]],"date-time":"2019-04-05T23:16:46Z","timestamp":1554506206000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0022149X07820220\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,9]]},"references-count":18,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2007,9]]}},"alternative-id":["S0022149X07820220"],"URL":"https:\/\/doi.org\/10.1017\/s0022149x07820220","relation":{},"ISSN":["0022-149X","1475-2697"],"issn-type":[{"value":"0022-149X","type":"print"},{"value":"1475-2697","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,9]]}}}