{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T16:52:00Z","timestamp":1767977520063,"version":"3.49.0"},"reference-count":20,"publisher":"Cambridge University Press (CUP)","issue":"4","license":[{"start":{"date-parts":[[2008,12,1]],"date-time":"2008-12-01T00:00:00Z","timestamp":1228089600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[2008,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Parasite strain characterization is essential for the establishment of a prevention and control strategy in any endemic area. The aim of this study was to characterize different <jats:italic>Echinococcus granulosus<\/jats:italic> isolates from Iran by using DNA sequences of the mitochondrial 12S rRNA gene. Thirty livers and lungs of cattle, sheep and goats naturally infected with <jats:italic>E. granulosus<\/jats:italic> were collected from abattoirs in northern and western Iran between June and October 2007. These samples yielded 18 fertile cysts which we used for the genetic work. We designed and tested two new primer pairs which specifically amplify portions of the mitochondrial 12S rRNA gene of the two strains (G1 and G6) of <jats:italic>E. granulosus<\/jats:italic> known to occur in Iran. One primer pair amplified a fragment of 259 base pairs (bp) from only the G1 strain. The second pair amplified a fragment of 676\u00a0bp from the G6 strain. The G1 genotype was identified in all fertile cyst samples, in agreement with previous studies in Iran. Ten of our samples and a single reference sample of the G6 strain were sequenced and compared with the G1 and G6 sequences deposited in GenBank.<\/jats:p>","DOI":"10.1017\/s0022149x0804594x","type":"journal-article","created":{"date-parts":[[2008,8,21]],"date-time":"2008-08-21T09:55:14Z","timestamp":1219312514000},"page":"343-347","source":"Crossref","is-referenced-by-count":31,"title":["<i>Echinococcus granulosus<\/i> strain differentiation in Iran based on sequence heterogeneity in the mitochondrial 12S rRNA gene"],"prefix":"10.1017","volume":"82","author":[{"given":"M.","family":"Rostami Nejad","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Nazemalhosseini Mojarad","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Z.","family":"Nochi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Fasihi Harandi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Cheraghipour","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.R.","family":"Mowlavi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.R.","family":"Zali","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2008,12,1]]},"reference":[{"key":"S0022149X0804594X_ref8","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-4017(99)00151-X"},{"key":"S0022149X0804594X_ref4","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-4017(02)00005-5"},{"key":"S0022149X0804594X_ref18","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182096008062"},{"key":"S0022149X0804594X_ref5","first-page":"25","article-title":"The epidemiology of hydatidosis with special reference to the Mediterranean area","volume":"33","author":"Euzeby","year":"1991","journal-title":"Parasitologia"},{"key":"S0022149X0804594X_ref13","first-page":"45","article-title":"A new primer pair in ITS1 region for molecular studies on Echinococcus granulosus","volume":"36","author":"Rahimi","year":"2007","journal-title":"Iranian Journal of Public Health"},{"key":"S0022149X0804594X_ref19","first-page":"595","volume-title":"Principles and practice of clinical parasitology","author":"Thompson","year":"2001"},{"key":"S0022149X0804594X_ref16","volume-title":"Molecular cloning, a laboratory manual","author":"Sambrook","year":"1989"},{"key":"S0022149X0804594X_ref1","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2005.01.005"},{"key":"S0022149X0804594X_ref11","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4017(94)90160-0"},{"key":"S0022149X0804594X_ref9","doi-asserted-by":"publisher","DOI":"10.2307\/3277581"},{"key":"S0022149X0804594X_ref2","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(92)90109-W"},{"key":"S0022149X0804594X_ref14","first-page":"29","article-title":"An analysis of hydatid cyst surgeries in patients referred to hospitals in Khorram-Abad, Lorestan during 2002\u201306","volume":"3","author":"Rostami Nejad","year":"2007","journal-title":"Iranian Journal of Parasitology"},{"key":"S0022149X0804594X_ref3","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-308X(08)60035-4"},{"key":"S0022149X0804594X_ref15","first-page":"59","article-title":"A survey on liver and lung hydatid cyst of slaughtered animals in Aleshtar. Lorestan province of Iran during 2002-06","volume":"2","author":"Rostami Nejad","year":"2007","journal-title":"Journal of Yasoj University of Medical Science"},{"key":"S0022149X0804594X_ref6","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182002002172"},{"key":"S0022149X0804594X_ref7","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182003003524"},{"key":"S0022149X0804594X_ref12","first-page":"205","volume-title":"Molecular taxonomy and strain analysis in Echinococcus","volume":"Vol. 341","author":"Pearson","year":"2002"},{"key":"S0022149X0804594X_ref17","first-page":"1488","volume-title":"Pediatric infectious diseases","author":"Schantz","year":"1997"},{"key":"S0022149X0804594X_ref20","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1998.59.171"},{"key":"S0022149X0804594X_ref10","first-page":"1018","article-title":"Helminthes recovered from the liver and lungs of camel with special reference to their incidence and pathogenesis in Shiraz, Islamic Republic of Iran","volume":"62","author":"Moghaddar","year":"1992","journal-title":"Indian Journal of Animal Science"}],"container-title":["Journal of Helminthology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0022149X0804594X","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,5]],"date-time":"2019-04-05T18:51:18Z","timestamp":1554490278000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0022149X0804594X\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,12]]},"references-count":20,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2008,12]]}},"alternative-id":["S0022149X0804594X"],"URL":"https:\/\/doi.org\/10.1017\/s0022149x0804594x","relation":{},"ISSN":["0022-149X","1475-2697"],"issn-type":[{"value":"0022-149X","type":"print"},{"value":"1475-2697","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,12]]}}}