{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T22:08:48Z","timestamp":1788300528345,"version":"build-2803163510"},"reference-count":26,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2008,6,1]],"date-time":"2008-06-01T00:00:00Z","timestamp":1212278400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[2008,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We estimated the prevalence of<jats:italic>Dirofilaria immitis<\/jats:italic>infection in domestic dogs in five Turkish provinces \u2013 Sakarya, Kocaeli, Ankara, Elazig and Mersin \u2013 using a commercial ELISA kit for detecting circulating antigen and a PCR test for detecting circulating microfilarial DNA. A total of 211 whole-blood and serum samples were collected from dogs of various breeds, ages and life status (owned or stray). Sample population characteristics were recorded and examined for differences in prevalence. Additionally, we collected 15 blood samples from cats (14 owned and 1 stray) from Ankara province and used PCR to detect<jats:italic>D. immitis<\/jats:italic>infection. Twenty-seven (12.8%) of 211 dog samples were positive for<jats:italic>D. immitis<\/jats:italic>antigen by ELISA. No differences in prevalence were observed by sex (female: 14.4%; male: 10.7%;<jats:italic>P<\/jats:italic>&gt;0.05). The prevalence of<jats:italic>D. immitis<\/jats:italic>infection varied with age: 11.8% in younger dogs (0.5\u20132 years) and 17.5% in older dogs (3\u20135 years). Prevalence between stray dogs (15.2%) and owned dogs (9.3%) did not differ (<jats:italic>P<\/jats:italic>&gt;0.05). Prevalence rates were highest in Kocaeli province (18.3%), followed by Ankara (14.8%), Sakarya (12.3%) and Mersin (10.5%) provinces. Prevalence in Elazig province was 0%. No dogs or cats had microfilarial DNA detectable by PCR.<\/jats:p>","DOI":"10.1017\/s0022149x0896079x","type":"journal-article","created":{"date-parts":[[2008,4,8]],"date-time":"2008-04-08T09:05:41Z","timestamp":1207645541000},"page":"181-186","source":"Crossref","is-referenced-by-count":19,"title":["Serological and molecular studies on<i>Dirofilaria immitis<\/i>in dogs from Turkey"],"prefix":"10.1017","volume":"82","author":[{"given":"S.","family":"Simsek","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.E.","family":"Utuk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Koroglu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Rishniw","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2008,6,1]]},"reference":[{"key":"S0022149X0896079X_ref22","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2005.03.033"},{"key":"S0022149X0896079X_ref21","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2005.10.013"},{"key":"S0022149X0896079X_ref20","first-page":"785","article-title":"Seroprevalence of Dirofilaria immitis in stray dogs in Istanbul and 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