{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T03:42:02Z","timestamp":1773286922790,"version":"3.50.1"},"reference-count":18,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2009,6,1]],"date-time":"2009-06-01T00:00:00Z","timestamp":1243814400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[2009,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In Spain, trichinellosis represents a public health problem, with an average of five outbreaks per year, wild boar meat being the main source of infection. A trichinellosis survey (2007\u20132008 hunting campaign) was carried out on wild boars in the Toledo Mountains (south-western Spain, EU) in the context of a surveillance programme on wildlife diseases. A total of 2216 wild boars from different locations of the region were examined. The examination was carried out by veterinarians in the local abattoir (Matadero Municipal de Toledo). The positive samples were sent to the Department of Parasitology (Facultad de Farmacia, UCM) for experimental isolation and specific identification by inter-simple sequence repeat-polymerase chain reaction (ISSR-PCR). Using this technique we identified 17 isolates as <jats:italic>Trichinella spiralis<\/jats:italic> with an electrophoretic profile indistinguishable from the <jats:italic>T. spiralis<\/jats:italic> reference strain (ISS48). We confirmed that ISSR-PCR is a robust technique for the molecular identification of <jats:italic>Trichinella<\/jats:italic> isolates. According to our results, the prevalence of <jats:italic>T. spiralis<\/jats:italic> in wild boars from the Toledo Mountains (&gt;800\u00a0m above sea level) during the hunting season was approximately 0.77%. The prevalence of <jats:italic>T. spiralis<\/jats:italic> (100% of our observations) is a good example of the persistence of this species in sylvatic conditions (coming from the domestic cycle), if a good wild host is abundant. Our observations confirm the major prevalence of <jats:italic>T. spiralis<\/jats:italic> over <jats:italic>T. britovi<\/jats:italic> in this region, as well as the risk to human health represented by the consumption of uninspected wild boar meat.<\/jats:p>","DOI":"10.1017\/s0022149x09349469","type":"journal-article","created":{"date-parts":[[2009,4,24]],"date-time":"2009-04-24T05:07:12Z","timestamp":1240549632000},"page":"117-120","source":"Crossref","is-referenced-by-count":12,"title":["Trichinellosis survey in the wild boar from the Toledo mountains in south-western Spain (2007\u20132008): molecular characterization of <i>Trichinella<\/i> isolates by ISSR-PCR"],"prefix":"10.1017","volume":"83","author":[{"given":"R.N.","family":"Garc\u00eda-S\u00e1nchez","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.J.","family":"Nogal-Ruiz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Manzano-Lorenzo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.M. Arroyo","family":"D\u00edaz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. P\u00e9rez","family":"L\u00f3pez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.J. S\u00e1nchez","family":"Ruano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. Ramiro","family":"Casas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. 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