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Total RNA was transcribed to cDNA by reverse transcription and an amplicon of 657\u00a0bp glutathione<jats:italic>S<\/jats:italic>-transferase gene was obtained by polymerase chain reaction (PCR). The present isolate showed 99.1% sequence homology with the published sequence of the<jats:italic>F. gigantica<\/jats:italic><jats:italic>GST<\/jats:italic>gene of cattle origin, with six nucleotide changes causing an overall change of four amino acids. Glutathione<jats:italic>S<\/jats:italic>-transferase protein was expressed in<jats:italic>Escherichia coli<\/jats:italic>using a prokaryotic expression vector pPROEXHT<jats:sup>b<\/jats:sup>. The recombinant protein was purified under non-denaturing and denaturing conditions by nickel nitrilotriacetic acid (Ni-NTA) affinity chromatography. Recombinant GST protein detected<jats:italic>F. gigantica<\/jats:italic>infection in naturally infected buffaloes by dot-ELISA.<\/jats:p>","DOI":"10.1017\/s0022149x09990046","type":"journal-article","created":{"date-parts":[[2009,7,21]],"date-time":"2009-07-21T09:56:03Z","timestamp":1248170163000},"page":"55-60","source":"Crossref","is-referenced-by-count":4,"title":["Molecular cloning and characterization of a glutathione<i>S<\/i>-transferase in the tropical liver fluke,<i>Fasciola 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