{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T07:26:04Z","timestamp":1790925964143,"version":"4.1.0"},"reference-count":19,"publisher":"Cambridge University Press (CUP)","issue":"1","license":[{"start":{"date-parts":[[2009,8,3]],"date-time":"2009-08-03T00:00:00Z","timestamp":1249257600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[2010,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Schistosoma haematobium<\/jats:italic> and <jats:italic>S. bovis<\/jats:italic> are widespread schistosome species causing human and cattle schistosomiasis, respectively, in Africa. The sympatric occurrence of these two species and their ability to infect the same <jats:italic>Bulinus<\/jats:italic> intermediate snail hosts necessitates precise methods of identification of the larval stages. A rapid diagnostic \u2018mulitplex\u2019 one-step polymerase chain reaction protocol (RD-PCR) was developed using cytochrome oxidase subunit 1 (COX1) mitochondrial DNA (mtDNA) to discriminate between <jats:italic>S. haematobium<\/jats:italic> and <jats:italic>S. bovis<\/jats:italic>. A single forward primer and two species-specific reverse primers were used to produce a polymerase chain reaction (PCR) fragment of 306\u00a0bp and 543\u00a0bp for <jats:italic>S. bovis<\/jats:italic> and <jats:italic>S. haematobium<\/jats:italic>, respectively. Serial dilutions were carried out on various lifecycle stages and species combinations to test the sensitivity and specificity of the primers. This RD-PCR proved highly sensitive, detecting a single larval stage and as little as 0.78\u00a0ng of genomic DNA (gDNA) from an adult schistosome, providing a cost-effective, rapid and robust molecular tool for high-throughput screening of <jats:italic>S. haematobium<\/jats:italic> and <jats:italic>S. bovis<\/jats:italic> populations. In areas where human and cattle schistosomiasis overlap and are transmitted in close proximity, this mitochondrial assay will be a valuable identification tool for epidemiological studies, especially when used in conjunction with other nuclear diagnostic markers.<\/jats:p>","DOI":"10.1017\/s0022149x09990447","type":"journal-article","created":{"date-parts":[[2009,8,3]],"date-time":"2009-08-03T09:05:07Z","timestamp":1249290307000},"page":"107-114","source":"Crossref","is-referenced-by-count":82,"title":["Rapid diagnostic multiplex PCR (RD-PCR) to discriminate <i>Schistosoma haematobium<\/i> and <i>S. bovis<\/i>"],"prefix":"10.1017","volume":"84","author":[{"given":"B.L.","family":"Webster","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Rollinson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.R.","family":"Stothard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Huyse","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2009,8,3]]},"reference":[{"key":"S0022149X09990447_ref20","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(80)90091-7"},{"key":"S0022149X09990447_ref19","first-page":"2","article-title":"Prevention and control of schistosomiasis and soil-transmitted helminthiasis: report of a WHO expert committee","volume":"912","year":"2002","journal-title":"World Health Organisation Technical 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