{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,2,6]],"date-time":"2023-02-06T13:30:53Z","timestamp":1675690253288},"reference-count":16,"publisher":"Cambridge University Press (CUP)","issue":"1","license":[{"start":{"date-parts":[[2010,3,26]],"date-time":"2010-03-26T00:00:00Z","timestamp":1269561600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[2011,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The importance of developing effective assays to diagnose, monitor and evaluate human lymphatic filariasis has been emphasized by the World Health Organization. Presently, few immunodiagnostics are available for filarial monitoring programmes. The <jats:italic>Wuchereria bancrofti (Wb)<\/jats:italic> SXP-1 parasite protein, with 84% homology to <jats:italic>Brugia malayi (Bm)<\/jats:italic> SXP-1, was found to be highly immunogenic. <jats:italic>Wb<\/jats:italic>SXP-1 is one among the diagnostic candidate molecules that were used for developing a rapid-antibody-flow-through diagnostic kit for filariasis. Studies were initiated with the aim of developing monoclonal antibodies against recombinant <jats:italic>Wb<\/jats:italic>SXP-1 and prospective applications for the detection of both circulating <jats:italic>Wb<\/jats:italic> and <jats:italic>Bm<\/jats:italic> antigens in serum samples from infected individuals. The monoclones 1A6C2 of subclass IgG1<jats:italic>k<\/jats:italic>, and 2A12F8 of class IgM, specifically detected <jats:italic>Wb<\/jats:italic> and <jats:italic>Bm<\/jats:italic> microfilaria isolated from patients and did not show cross-reactivity with other filarial recombinant antigens. We anticipate that this work will address the problems faced in the rapid diagnosis of human lymphatic filariasis in endemic areas in developing countries.<\/jats:p>","DOI":"10.1017\/s0022149x10000118","type":"journal-article","created":{"date-parts":[[2010,3,26]],"date-time":"2010-03-26T05:56:25Z","timestamp":1269582985000},"page":"1-6","source":"Crossref","is-referenced-by-count":8,"title":["Development and characterization of monoclonal antibodies against <i>Wb<\/i>SXP-1 for the detection of circulating filarial antigens"],"prefix":"10.1017","volume":"85","author":[{"given":"S.","family":"Janardhan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Pandiaraja","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V.","family":"Pandey","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Karande","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Kaliraj","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2010,3,26]]},"reference":[{"key":"S0022149X10000118_ref2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.179.13.4403-4406.1997"},{"key":"S0022149X10000118_ref3","doi-asserted-by":"publisher","DOI":"10.1089\/153685902760213895"},{"key":"S0022149X10000118_ref8","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2883-3-9"},{"key":"S0022149X10000118_ref7","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.2002.tb02703.x"},{"key":"S0022149X10000118_ref4","volume-title":"Using antibodies: a laboratory manual","author":"Harlow","year":"1998"},{"key":"S0022149X10000118_ref5","doi-asserted-by":"publisher","DOI":"10.1007\/s10295-007-0243-3"},{"key":"S0022149X10000118_ref10","first-page":"403","article-title":"A highly specific and sensitive monoclonal antibody-based ELISA for the detection of circulating antigen in bancroftian filariasis","volume":"41","author":"More","year":"1990","journal-title":"Tropical Medicine and Parasitology"},{"key":"S0022149X10000118_ref9","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2004.04.002"},{"key":"S0022149X10000118_ref16","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.9.4350"},{"key":"S0022149X10000118_ref11","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3156.2000.00620.x"},{"key":"S0022149X10000118_ref1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.2004.tb03547.x"},{"key":"S0022149X10000118_ref15","doi-asserted-by":"publisher","DOI":"10.1080\/00034983.2000.11813582"},{"key":"S0022149X10000118_ref17","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-4758(97)01130-7"},{"key":"S0022149X10000118_ref14","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-6851(99)00231-5"},{"key":"S0022149X10000118_ref13","first-page":"304","article-title":"Comparison of an immunochromatographic card test with night blood smear examination for detection of Wuchereriabancrofti microfilaria carriers","volume":"17","author":"Pani","year":"2004","journal-title":"National Medical Journal of India"},{"key":"S0022149X10000118_ref12","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3156.2000.00559.x"}],"container-title":["Journal of Helminthology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0022149X10000118","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,27]],"date-time":"2019-04-27T15:27:38Z","timestamp":1556378858000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0022149X10000118\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,3,26]]},"references-count":16,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2011,3]]}},"alternative-id":["S0022149X10000118"],"URL":"https:\/\/doi.org\/10.1017\/s0022149x10000118","relation":{},"ISSN":["0022-149X","1475-2697"],"issn-type":[{"value":"0022-149X","type":"print"},{"value":"1475-2697","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,3,26]]}}}