{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T10:57:17Z","timestamp":1790506637559,"version":"4.1.0"},"reference-count":62,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2011,1,13]],"date-time":"2011-01-13T00:00:00Z","timestamp":1294876800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[2011,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The immune response of mice experimentally infected with<jats:italic>Echinococcus multilocularis<\/jats:italic>metacestodes becomes impaired so as to allow parasite survival and proliferation. Our study tackled the question on how different classes of<jats:italic>E. multilocularis<\/jats:italic>antigens (crude vesicular fluid (VF); purified proteinic rec-14-3-3; purified carbohydrate Em2(G11)) are involved in the maturation process of bone-marrow-derived dendritic cells (BMDCs) and subsequent exposure to lymph node (LN) cells. In our experiments, we used BMDCs cultivated from either na\u00efve (control) or alveolar echinococcosis (AE)-infected C57BL\/6 mice. We then tested surface markers (CD80, CD86, MHC class II) and cytokine expression levels (interleukin (IL)-10, IL-12p40 and tumour necrosis factor (TNF)-\u03b1) of non-stimulated BMDCs versus BMDCs stimulated with different Em-antigens or lipopolysaccharide (LPS). While LPS and rec-14-3-3-antigen were able to induce CD80, CD86 and (to a lower extent) MHC class II surface expression, Em2(G11) and, strikingly, also VF-antigen failed to do so. Similarly, LPS and rec-14-3-3 yielded elevated IL-12, TNF-\u03b1 and IL-10 expression levels, while Em2(G11) and VF-antigen didn't. When na\u00efve BMDCs were loaded with VF-antigen, they induced a strong non-specific proliferation of uncommitted LN cells. For both, BMDCs or LN cells, isolated from AE-infected mice, proliferation was abrogated. The most striking difference, revealed by comparing na\u00efve with AE-BMDCs, was the complete inability of LPS-stimulated AE-BMDCs to activate lymphocytes from any LN cell group. Overall, the presenting activity of BMDCs from AE-infected mice seemed to trigger unresponsiveness in T cells, especially in the case of VF-antigen stimulation, thus contributing to the suppression of clonal expansion during the chronic phase of AE infection.<\/jats:p>","DOI":"10.1017\/s0022149x10000878","type":"journal-article","created":{"date-parts":[[2011,1,13]],"date-time":"2011-01-13T14:42:25Z","timestamp":1294929745000},"page":"128-137","source":"Crossref","is-referenced-by-count":11,"title":["<i>In vitro<\/i>induction of lymph node cell proliferation by mouse bone marrow dendritic cells following stimulation with different<i>Echinococcus 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