{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T10:52:34Z","timestamp":1784890354969,"version":"3.55.0"},"reference-count":39,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2012,3,13]],"date-time":"2012-03-13T00:00:00Z","timestamp":1331596800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[2013,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The 14.5\u00a0kDa fatty acid binding protein (FABP) was isolated from the crude extract of adult<jats:italic>Fasciola gigantica<\/jats:italic>worms. Polyclonal anti-FABP IgG was generated in rabbits immunized with prepared FABP antigen. Sandwich enzyme-linked immunosorbent assay (ELISA) was applied to detect coproantigen in stools and circulating<jats:italic>Fasciola<\/jats:italic>antigen (CA) in sera of 126 water buffaloes by using purified and horseradish peroxidase (HRP)-conjugated anti-FABP IgG. Sandwich ELISA sensitivity was 96.97% and 94.95%; while specificity was 94.12% and 82.35% for coproantigen and CA detection, respectively. However, sensitivity and specificity of the Kato\u2013Katz technique was 73.74% and 100%, respectively. The diagnostic efficacy of sandwich ELISA was 96.55% and 93.1% for coproantigen and CA detection, respectively. In contrast, the diagnostic efficacy of the Kato\u2013Katz technique was 77.59%. In conclusion, these results demonstrate that the purified 14.5\u00a0kDa FABP provides a more suitable antigen for immunodiagnosis of early and current bubaline fascioliasis by using sandwich ELISA.<\/jats:p>","DOI":"10.1017\/s0022149x12000168","type":"journal-article","created":{"date-parts":[[2012,3,13]],"date-time":"2012-03-13T10:39:19Z","timestamp":1331635159000},"page":"147-153","source":"Crossref","is-referenced-by-count":5,"title":["Diagnostic potential of<i>Fasciola gigantica<\/i>-derived 14.5\u00a0kDa fatty acid binding protein in the immunodiagnosis of bubaline fascioliasis"],"prefix":"10.1017","volume":"87","author":[{"given":"G.","family":"Allam","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.R.","family":"Bauomy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Z.M.","family":"Hemyeda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.M.","family":"Diab","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.F.","family":"Sakran","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2012,3,13]]},"reference":[{"key":"S0022149X12000168_ref8","doi-asserted-by":"crossref","first-page":"2637","DOI":"10.1128\/JCM.28.12.2637-2640.1990","article-title":"Detection of circulating excretory antigens in human fascioliasis by sandwich enzyme-linked immunosorbent assay","volume":"28","author":"Espino","year":"1990","journal-title":"Journal of Clinical Microbiology"},{"key":"S0022149X12000168_ref5","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1128\/CDLI.8.1.174-177.2001","article-title":"Immunodiagnosis of human fascioliosis by an Enzyme-Linked Immunosorbent Assay (ELISA) and a micro-ELISA","volume":"8","author":"Carnevale","year":"2001","journal-title":"Clinical and Diagnostic Laboratory Immunology"},{"key":"S0022149X12000168_ref9","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(92)90174-B"},{"key":"S0022149X12000168_ref3","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-4017(99)00026-6"},{"key":"S0022149X12000168_ref1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3024.1999.00191.x"},{"key":"S0022149X12000168_ref24","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-005-1397-4"},{"key":"S0022149X12000168_ref2","article-title":"Evaluation of a 14.5\u00a0kDa-Fasciola gigantica fatty acid binding protein as a diagnostic antigen for human fascioliasis","author":"Allam","year":"2011","journal-title":"Parasitology Research"},{"key":"S0022149X12000168_ref27","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1998.58.417"},{"key":"S0022149X12000168_ref4","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"S0022149X12000168_ref29","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2006.03.004"},{"key":"S0022149X12000168_ref30","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-4017(01)00388-0"},{"key":"S0022149X12000168_ref6","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3156.1997.d01-264.x"},{"key":"S0022149X12000168_ref11","doi-asserted-by":"publisher","DOI":"10.1079\/JOH2005303"},{"key":"S0022149X12000168_ref32","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3156.1999.00467.x"},{"key":"S0022149X12000168_ref7","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1128\/JCM.32.1.190-193.1994","article-title":"Sandwich enzyme-liked immunosorbent assay for detection of excretory-secretory antigens in humans with fascioliasis","volume":"32","author":"Espino","year":"1994","journal-title":"Journal of Clinical Microbiology"},{"key":"S0022149X12000168_ref10","doi-asserted-by":"crossref","first-page":"2723","DOI":"10.1128\/JCM.36.9.2723-2726.1998","article-title":"Dynamics of antigenemia and coproantigens during a human Fasciola hepatica outbreak","volume":"36","author":"Espino","year":"1998","journal-title":"Journal of Clinical Microbiology"},{"key":"S0022149X12000168_ref13","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4017(94)00718-R"},{"key":"S0022149X12000168_ref17","first-page":"115","article-title":"The diagnostic efficacy of Fasciola gigantica coproantigen in naturally infected cattle and buffaloes","volume":"38","author":"Hassan","year":"2008","journal-title":"Journal of the Egyptian Society of Parasitology"},{"key":"S0022149X12000168_ref39","first-page":"193","volume-title":"in Immunology: Theoretical and practical concepts in laboratory medicine","author":"Zane","year":"2001"},{"key":"S0022149X12000168_ref14","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-4017(96)01056-4"},{"key":"S0022149X12000168_ref12","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-7519(97)00096-9"},{"key":"S0022149X12000168_ref34","volume-title":"Statistical methods","author":"Snedecor","year":"1981"},{"key":"S0022149X12000168_ref16","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-4017(96)01065-5"},{"key":"S0022149X12000168_ref18","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4612-3898-0_90"},{"key":"S0022149X12000168_ref19","doi-asserted-by":"publisher","DOI":"10.1079\/JOH2005304"},{"key":"S0022149X12000168_ref20","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"S0022149X12000168_ref21","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(87)90324-3"},{"key":"S0022149X12000168_ref22","doi-asserted-by":"publisher","DOI":"10.1645\/GE-925R.1"},{"key":"S0022149X12000168_ref23","first-page":"379","article-title":"Role of ELISA in early detection of Fasciola copro-antigens in experimentally infected animals","volume":"28","author":"Moustafa","year":"1998","journal-title":"Journal of the Egyptian Society of Parasitology"},{"key":"S0022149X12000168_ref25","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-67356-6"},{"key":"S0022149X12000168_ref26","doi-asserted-by":"publisher","DOI":"10.1007\/BF00231361"},{"key":"S0022149X12000168_ref28","first-page":"40","article-title":"Evaluation of different antigens extracted from Fasciola gigantica for effective specific diagnosis of fascioliasis","volume":"36","author":"Rabia","year":"2007","journal-title":"New Egyptian Journal of Medicine"},{"key":"S0022149X12000168_ref31","first-page":"145","article-title":"Ion-exchange chromatography","volume":"59","author":"Sheehan","year":"1996","journal-title":"Methods in Molecular Biology"},{"key":"S0022149X12000168_ref33","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-4017(02)00007-9"},{"key":"S0022149X12000168_ref35","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.1.269"},{"key":"S0022149X12000168_ref37","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4347(91)80212-U"},{"key":"S0022149X12000168_ref38","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(91)90300-N"},{"key":"S0022149X12000168_ref36","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(84)90243-4"},{"key":"S0022149X12000168_ref15","doi-asserted-by":"publisher","DOI":"10.1016\/0001-706X(93)90009-Z"}],"container-title":["Journal of Helminthology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0022149X12000168","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,7,4]],"date-time":"2020-07-04T04:06:03Z","timestamp":1593835563000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0022149X12000168\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,3,13]]},"references-count":39,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2013,6]]}},"alternative-id":["S0022149X12000168"],"URL":"https:\/\/doi.org\/10.1017\/s0022149x12000168","relation":{},"ISSN":["0022-149X","1475-2697"],"issn-type":[{"value":"0022-149X","type":"print"},{"value":"1475-2697","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,3,13]]}}}