{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T12:32:21Z","timestamp":1777638741982,"version":"3.51.4"},"reference-count":38,"publisher":"Cambridge University Press (CUP)","issue":"3","license":[{"start":{"date-parts":[[2013,3,19]],"date-time":"2013-03-19T00:00:00Z","timestamp":1363651200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[2014,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The main goal of the current work was to develop and validate an<jats:italic>in vitro<\/jats:italic>fluke egg hatch test, as a method for the detection of albendazole (ABZ) resistance in the liver fluke,<jats:italic>Fasciola hepatica<\/jats:italic>. Fluke eggs (200\/ml,<jats:italic>n<\/jats:italic>=\u00a05) from six different isolates were used in the current experimental work. They were obtained from different geographical locations and named Cullompton (UK), CEDIVE (Chascomus, Argentina), INTA-Bariloche (Bariloche, Argentina), Rubino (Uruguay), Cajamarca (Per\u00fa) and R\u00edo Chico (Catamarca, Argentina). The fluke eggs were incubated (25\u00b0C) for a 12-h period in the presence of either ABZ or its sulphoxide metabolite (ABZ.SO) (5, 0.5 or 0.05\u00a0nmol\/ml). Untreated eggs were incubated as a control. Incubated eggs (with or without drug present) were kept in darkness at 25\u00b0C for 15 days. Afterwards, the trematode eggs were exposed to daylight over a 2-h period. Hatched and unhatched eggs were evaluated using an optical microscope, and the ovicidal activity was assessed for each fluke isolate. A very low ovicidal activity (\u00a0\u2264\u00a013.4%) was observed in the ABZ-resistant CEDIVE isolate for both ABZ and ABZ.SO. Conversely, in the INTA-Bariloche and R\u00edo Chico isolates, which are suspected to be susceptible to ABZ, ovicidal activities\u00a0\u2265\u00a070.3% were observed after incubation with ABZ at the lowest concentration tested (0.05\u00a0nmol\/ml). This finding correlates with that previously described for the ABZ-susceptible Cullompton. Finally, the Cajamarca and Rubino isolates behaved as ABZ resistant, since no ovicidal activity was observed after eggs were incubated with ABZ at 0.5\u00a0nmol\/ml. Considering the specific results obtained for each isolate under assessment, the egg hatch test described here may be a suitable method for detection of ABZ resistance in<jats:italic>F. hepatica<\/jats:italic>.<\/jats:p>","DOI":"10.1017\/s0022149x13000163","type":"journal-article","created":{"date-parts":[[2013,3,19]],"date-time":"2013-03-19T14:07:12Z","timestamp":1363702032000},"page":"286-292","source":"Crossref","is-referenced-by-count":40,"title":["Testing albendazole resistance in<i>Fasciola hepatica<\/i>: validation of an egg hatch test with isolates from South America and the United Kingdom"],"prefix":"10.1017","volume":"88","author":[{"given":"J.","family":"Canevari","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Ceballos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Sanabria","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Romero","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"Olaechea","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Ortiz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Cabrera","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V.","family":"Gayo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I.","family":"Fairweather","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Lanusse","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Alvarez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2013,3,19]]},"reference":[{"key":"S0022149X13000163_ref14","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2010.10.037"},{"key":"S0022149X13000163_ref29","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2010.12.047"},{"key":"S0022149X13000163_ref11","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2010.12.011"},{"key":"S0022149X13000163_ref24","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182006000114"},{"key":"S0022149X13000163_ref7","doi-asserted-by":"publisher","DOI":"10.1136\/vr.103.16.360"},{"key":"S0022149X13000163_ref3","doi-asserted-by":"publisher","DOI":"10.1136\/vr.113.14.315"},{"key":"S0022149X13000163_ref28","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-4017(00)00267-3"},{"key":"S0022149X13000163_ref22","doi-asserted-by":"crossref","first-page":"1126","DOI":"10.2460\/ajvr.1980.41.07.1126","article-title":"Pharmacokinetics 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(2011b). An experimental study in sheep, to confirm triclabendazole resistance of Fasciola hepatica in Patagonia, Argentina. 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