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Helminthol."],"published-print":{"date-parts":[[2014,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Dichloromethane and aqueous fractions from leaves and stems of <jats:italic>Piper arboreum<\/jats:italic> Aubl., <jats:italic>P. aduncum<\/jats:italic> L., <jats:italic>P. amalago<\/jats:italic> L., <jats:italic>P. crassinervium<\/jats:italic> H.B. &amp; K., <jats:italic>P. diospyrifolium<\/jats:italic> Kunth, <jats:italic>P. hispidum<\/jats:italic> Sw. and <jats:italic>P. xylosteoides<\/jats:italic> (Kunth) Steud. were tested against adult worms of <jats:italic>Schistosoma mansoni<\/jats:italic>. The <jats:italic>in vitro<\/jats:italic> activity was evaluated in terms of mortality, number of separated worms and number of worms with reduced motor activity. Most dichloromethane fractions from all <jats:italic>Piper<\/jats:italic> species showed moderate schistosomicidal activity, but aqueous fractions were not active. The dichloromethane fraction of <jats:italic>P. amalago<\/jats:italic> leaves (at 100\u00a0<jats:roman>\u03bc<\/jats:roman>g\/ml) showed the highest activity, resulting in worm mortality, the separation of worm pairs and reduced motor activity. Chromatographic fractionation of the dichloromethane fraction of <jats:italic>P. amalago<\/jats:italic> leaves led to the isolation of its major compound, which was also tested against adults of <jats:italic>S. mansoni<\/jats:italic>. The isolated piperamide <jats:italic>N<\/jats:italic>-[7-(3\u2032,4\u2032-methylenedioxyphenyl)-2(<jats:italic>Z<\/jats:italic>),4(<jats:italic>Z<\/jats:italic>)-heptadienoyl] pyrrolidine, at 100\u00a0<jats:roman>\u03bc<\/jats:roman><jats:sc>m<\/jats:sc>, resulted in the mortality of all adult worms after 24\u00a0h of incubation. The findings suggest that species of <jats:italic>Piper<\/jats:italic> are potential sources of schistosomicidal compounds.<\/jats:p>","DOI":"10.1017\/s0022149x13000205","type":"journal-article","created":{"date-parts":[[2013,4,8]],"date-time":"2013-04-08T09:46:37Z","timestamp":1365414397000},"page":"321-326","source":"Crossref","is-referenced-by-count":23,"title":["<i>In vitro<\/i> schistosomicidal effects of aqueous and dichloromethane fractions from leaves and stems of <i>Piper<\/i> species and the isolation of an active amide from <i>P. amalago<\/i> L. (Piperaceae)"],"prefix":"10.1017","volume":"88","author":[{"given":"V.S.","family":"Carrara","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.C.H.","family":"Vieira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.G.","family":"de Paula","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V.","family":"Rodrigues","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.G.","family":"Magalh\u00e3es","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.A.G.","family":"Cortez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.A.","family":"Da Silva Filho","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2013,4,8]]},"reference":[{"key":"S0022149X13000205_ref22","doi-asserted-by":"publisher","DOI":"10.1590\/S0102-695X2009000200003"},{"key":"S0022149X13000205_ref18","unstructured":"Patent 0503951-7 (2008) Process to obtain synthetic and semi-synthetic lignan derivatives, their antiparasitic activities and corresponding pharmaceutical formulations, including the therapeutic method using said lignan for the treatment of parasitosis. 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