{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T20:16:41Z","timestamp":1779913001931,"version":"3.53.1"},"reference-count":23,"publisher":"Cambridge University Press (CUP)","issue":"1","license":[{"start":{"date-parts":[[2013,5,13]],"date-time":"2013-05-13T00:00:00Z","timestamp":1368403200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[2015,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Nematodes of the genus <jats:italic>Trichinella<\/jats:italic> which infect wildlife and domestic animals show a cosmopolitan distribution. These zoonotic parasites are the aetiological agents of a severe human disease, trichinellosis. Twelve taxa are recognized in the <jats:italic>Trichinella<\/jats:italic> genus, but they cannot be identified by morphology since they are sibling species\/genotypes. For epidemiological studies, it is extremely important to identify each taxon since they have different distribution areas and host ranges. In the present study, polymerase chain reaction (PCR) amplification of the mitochondrial large subunit ribosomal RNA (lsu-RNA) gene coupled with a pyrosequencing technique was developed to distinguish among four <jats:italic>Trichinella<\/jats:italic> species: <jats:italic>Trichinella spiralis<\/jats:italic>, <jats:italic>T. pseudospiralis<\/jats:italic>, <jats:italic>T. papuae<\/jats:italic> and <jats:italic>T. zimbabwensis<\/jats:italic>. A PCR method was used to amplify the lsu-RNA of <jats:italic>Trichinella<\/jats:italic> sp. larvae in mouse muscles and single larvae collected from infected muscles by digestion. The results show that the four <jats:italic>Trichinella<\/jats:italic> species can be distinguished by using 26 nucleotides in the target region and the method is sensitive enough to identify individual larvae. The pyrosequencing provides a simple, rapid and high-throughput tool for the differentiation of <jats:italic>Trichinella<\/jats:italic> species.<\/jats:p>","DOI":"10.1017\/s0022149x13000308","type":"journal-article","created":{"date-parts":[[2013,5,13]],"date-time":"2013-05-13T08:32:14Z","timestamp":1368433934000},"page":"118-123","source":"Crossref","is-referenced-by-count":5,"title":["Molecular differentiation of <i>Trichinella spiralis<\/i>, <i>T. pseudospiralis<\/i>, <i>T. papuae<\/i> and <i>T. zimbabwensis<\/i> by pyrosequencing"],"prefix":"10.1017","volume":"89","author":[{"given":"L.","family":"Sadaow","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Tantrawatpan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.M.","family":"Intapan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.","family":"Lulitanond","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Boonmars","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Morakote","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Pozio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W.","family":"Maleewong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2013,5,13]]},"reference":[{"key":"S0022149X13000308_ref17","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2005.03.017"},{"key":"S0022149X13000308_ref8","doi-asserted-by":"publisher","DOI":"10.1086\/516278"},{"key":"S0022149X13000308_ref5","doi-asserted-by":"publisher","DOI":"10.1006\/mcpr.1997.0142"},{"key":"S0022149X13000308_ref23","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-7519(99)00107-1"},{"key":"S0022149X13000308_ref14","first-page":"73","article-title":"Trichinellosis 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