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Helminthol."],"published-print":{"date-parts":[[2014,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A rapid dot immunogold filtration assay (DIGFA) was adopted for specific immunodiagnosis of human cerebral angiostrongyliasis, using purified 31-kDa glycoprotein specific to<jats:italic>Angiostrongylus cantonensis<\/jats:italic>as diagnostic antigen and protein A colloidal gold conjugate as antigen\u2013antibody detector. A total of 59 serum samples were assayed \u2013 11 samples from clinically diagnosed patients with detectable<jats:italic>A. cantonensis<\/jats:italic>-specific antibody in immunoblotting; 23 samples from patients with other related parasitic diseases, i.e. gnathostomiasis (<jats:italic>n<\/jats:italic>=\u00a08), cysticercosis (<jats:italic>n<\/jats:italic>=\u00a05), toxocariasis (<jats:italic>n<\/jats:italic>=\u00a02), filariasis (<jats:italic>n<\/jats:italic>=\u00a04), paragonimiasis (<jats:italic>n<\/jats:italic>=\u00a02) and malaria (<jats:italic>n<\/jats:italic>=\u00a02); and 25 samples from normal healthy subjects. The sensitivity and specificity of DIGFA to detect anti-<jats:italic>A. cantonensis<\/jats:italic>specific antibodies in serologically confirmed angiostrongyliasis cases, were both 100%. No positive DIGFA was observed in cases with other parasitic diseases, and the healthy control subjects. The 3-min DIGFA is as sensitive and specific as the 3-h immunoblot test in angiostrongyliasis confirmed cases that revealed a 31-kDa reactive band. The gold-based DIGFA is more rapid and easier to perform than the traditional enzyme-linked immunosorbent assay (ELISA). The test utilizing purified<jats:italic>A<\/jats:italic>.<jats:italic>cantonensis<\/jats:italic>antigen is reliable and reproducible for specific immunodiagnosis of human infection with<jats:italic>A. cantonensis<\/jats:italic>\u2013 thus can be applied as an additional routine test for clinical diagnostic support. Large-scale sero-epidemiological studies in endemic communities in north-east Thailand are under way to evaluate its usefulness under field conditions.<\/jats:p>","DOI":"10.1017\/s0022149x13000321","type":"journal-article","created":{"date-parts":[[2013,5,28]],"date-time":"2013-05-28T08:19:45Z","timestamp":1369729185000},"page":"396-401","source":"Crossref","is-referenced-by-count":17,"title":["Dot immunogold filtration assay (DIGFA) for the rapid detection of specific antibodies against the rat lungworm<i>Angiostrongylus cantonensis<\/i>(Nematoda: Metastrongyloidea) using purified 31-kDa 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PhD Thesis, University of Malaya."},{"key":"S0022149X13000321_ref3","first-page":"37","article-title":"The place of gold in rapid tests","volume":"6","author":"Chandler","year":"2000","journal-title":"IVD Technology"},{"key":"S0022149X13000321_ref11","first-page":"308","article-title":"Purification of a specific immunodiagnostic Parastrongyluscantonensis antigen by electroelution from SDS-polyacrylamide gels","volume":"32","author":"Eamsobhana","year":"2001","journal-title":"Southeast Asian Journal of Tropical Medicine and Public Health"},{"key":"S0022149X13000321_ref23","first-page":"97","article-title":"Application of dot immunogold filtration assay for detecting serum antibodies in clonorchiasis patients","volume":"19","author":"Liu","year":"2001","journal-title":"Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi"},{"key":"S0022149X13000321_ref2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(84)90518-9"},{"key":"S0022149X13000321_ref7","author":"Eamsobhana","year":"2006"},{"key":"S0022149X13000321_ref13","doi-asserted-by":"publisher","DOI":"10.1079\/JOH2004257"},{"key":"S0022149X13000321_ref8","first-page":"84","article-title":"Towards precise and rapid diagnosis of eosinophilic meningitis due to the rat lungworm Angiostrongylus cantonensis","volume":"62","author":"Eamsobhana","year":"2010","journal-title":"Siriraj Medical Journal"},{"key":"S0022149X13000321_ref12","first-page":"1","article-title":"Dot-blot ELISA for the immunological detection of specific antibody to Parastrongylus cantonensis","volume":"20","author":"Eamsobhana","year":"2003","journal-title":"Tropical Biomedicine"}],"container-title":["Journal of Helminthology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0022149X13000321","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,7,27]],"date-time":"2020-07-27T05:50:56Z","timestamp":1595829056000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0022149X13000321\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,5,28]]},"references-count":31,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2014,12]]}},"alternative-id":["S0022149X13000321"],"URL":"https:\/\/doi.org\/10.1017\/s0022149x13000321","relation":{},"ISSN":["0022-149X","1475-2697"],"issn-type":[{"value":"0022-149X","type":"print"},{"value":"1475-2697","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,5,28]]}}}