{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T12:57:52Z","timestamp":1792069072266,"version":"4.4.0"},"reference-count":28,"publisher":"Cambridge University Press (CUP)","issue":"1","license":[{"start":{"date-parts":[[2013,7,26]],"date-time":"2013-07-26T00:00:00Z","timestamp":1374796800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[2015,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The genetic variations in internal transcribed spacers (ITS) spanning ITS-1, 5.8S and ITS-2 rDNA of<jats:italic>Dicrocoelium dendriticum<\/jats:italic>, isolated from sheep and goats in four geographical regions in Shaanxi province, were examined. The lengths of ITS-1, 5.8S and ITS-2 rDNA sequences for<jats:italic>D. dendriticum<\/jats:italic>were 749\u00a0bp, 161\u00a0bp and 234\u00a0bp, respectively. Intra-specific sequence variations of<jats:italic>D. dendriticum<\/jats:italic>were 0\u20130.5% for ITS-1 and 0\u20131.3% for ITS-2 rDNA, while the inter-specific variations among species in genus<jats:italic>Dicrocoelium<\/jats:italic>in ITS-2 rDNA were 3.4\u201312.3%. Phylogenetic analysis based on sequences of ITS-2 rDNA showed that all<jats:italic>D. dendriticum<\/jats:italic>isolates in the present study were grouped with reference<jats:italic>D. dendriticum<\/jats:italic>isolates from sheep and goats, and<jats:italic>D. dendriticum<\/jats:italic>isolates from cattle and Japanese serow were clustered in a sister clade. However, the phylogenetic tree could not reveal geographically genetic relationships of<jats:italic>D. dendriticum<\/jats:italic>isolates in different origins and hosts. These findings provided basic information for further study of molecular epidemiology and control of<jats:italic>D. dendriticum<\/jats:italic>infection in Shaanxi province as well as in the world.<\/jats:p>","DOI":"10.1017\/s0022149x13000503","type":"journal-article","created":{"date-parts":[[2013,7,26]],"date-time":"2013-07-26T15:12:09Z","timestamp":1374851529000},"page":"124-129","source":"Crossref","is-referenced-by-count":16,"title":["Characterization of<i>Dicrocoelium dendriticum<\/i>isolates from small ruminants in Shaanxi Province, north-western China, using internal transcribed spacers of nuclear ribosomal 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