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Helminthol."],"published-print":{"date-parts":[[2015,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The present study assessed whether the genetic variation among different hosts (sheep and cattle) and geographical isolates (<jats:italic>n<\/jats:italic>=\u00a028) of<jats:italic>Dicrocoelium dendriticum<\/jats:italic>from Iran is present based on mitochondrial (<jats:italic>nad<\/jats:italic>1) and ribosomal (ITS-2) DNA markers. Molecular analysis revealed the presence of at least ten and two distinct haplotypes in the NADH dehydrogenase gene (<jats:italic>nad<\/jats:italic>1) and internal transcribed spacer 2 (ITS-2), respectively. The<jats:italic>nad<\/jats:italic>1 and ITS-2 sequence data were deposited in GenBank under accession numbers, JX050110\u2013134 and JQ966972\u20133. According to the results of our study, ND-D and ITS-A are established as being the predominant haplotypes of<jats:italic>D. dendriticum<\/jats:italic>in Iran. The Iranian isolates showed a higher intraspecific genetic diversity of 0\u20130.97% for<jats:italic>nad<\/jats:italic>1, compared to 0\u20130.42% for ITS-2. The alignment and comparison of<jats:italic>nad<\/jats:italic>1 and ITS-2 sequences revealed eight and one polymorphic sites, respectively. In the<jats:italic>nad<\/jats:italic>1 sequences, six were silent and two nucleotide substitutions were responsible for amino acid alterations. A phylogenetic analysis of the sequence data revealed that host associations and geographic location are not likely useful markers for<jats:italic>D. dendriticum<\/jats:italic>haplotype classification. Consequently, sequencing results obtained from the<jats:italic>nad1<\/jats:italic>gene as a mitochondrial marker for the first time in this study would provide a valuable tool to analyse further molecular details of<jats:italic>D. dendriticum<\/jats:italic>worldwide.<\/jats:p>","DOI":"10.1017\/s0022149x13000679","type":"journal-article","created":{"date-parts":[[2013,10,11]],"date-time":"2013-10-11T16:28:35Z","timestamp":1381508915000},"page":"158-164","source":"Crossref","is-referenced-by-count":15,"title":["Characterization of<i>Dicrocoelium dendriticum<\/i>haplotypes from sheep and cattle in Iran based on the internal transcribed spacer 2 (ITS-2) and NADH dehydrogenase gene 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