{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T09:17:22Z","timestamp":1761988642315},"reference-count":35,"publisher":"Cambridge University Press (CUP)","issue":"1","license":[{"start":{"date-parts":[[2013,10,28]],"date-time":"2013-10-28T00:00:00Z","timestamp":1382918400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[2015,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Taeniid infections in intermediate hosts manifest themselves as extraintestinal larval stages which, in early development, lack species-specific characteristics. The inability to distinguish infections of zoonotic importance such as <jats:italic>Echinococcus multilocularis<\/jats:italic> from other taeniid infections that have mainly veterinary significance stimulated the development of species-specific molecular diagnostics. In this study, the prevalence of taeniid infections in potential intermediate hosts was evaluated using both morphological diagnosis and a newly described multiplex polymerase chain reaction (PCR) for species determination. Small mammals (<jats:italic>N<\/jats:italic>=\u00a0719) were trapped in three different types of habitats in north-east Zealand, Denmark. The sensitivity of the multiplex PCR (90.5%) exceeded that of morphological examination (57.9%) for identifying 95 taeniid infections. The use of the multiplex PCR resulted in higher prevalence rates due to improved detection of immature liver infections with <jats:italic>Hydatigera taeniaeformis<\/jats:italic> and <jats:italic>Versteria mustelae,<\/jats:italic> but did not affect the observed prevalence rates of peritoneal metacestodes of <jats:italic>Taenia polyacantha<\/jats:italic>. The prevalence of taeniid infections showed a significant difference according to habitat type, potentially identifying a \u2018sylvatic\u2019 transmission and an \u2018urban\u2019 transmission, with marked variation among different taeniid species. <jats:italic>Versteria mustelae<\/jats:italic> and <jats:italic>T. polyacantha<\/jats:italic> were more prevalent in rural forests, while infections with <jats:italic>H. taeniaeformis<\/jats:italic> were dominant in urban parks\/forests and in residential and farm gardens. The multiplex PCR facilitated a better utilization of wildlife samples by yielding a higher number of definitive diagnoses of ambiguous taeniid infections in liver lesions, allowing for more accurate epidemiological data and, hence, a more accurate risk assessment.<\/jats:p>","DOI":"10.1017\/s0022149x13000680","type":"journal-article","created":{"date-parts":[[2013,10,28]],"date-time":"2013-10-28T06:49:40Z","timestamp":1382942980000},"page":"112-117","source":"Crossref","is-referenced-by-count":7,"title":["Morphological and molecular analyses of larval taeniid species in small mammals from contrasting habitats in 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