{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T10:04:58Z","timestamp":1780394698447,"version":"3.54.1"},"reference-count":33,"publisher":"Cambridge University Press (CUP)","issue":"4","license":[{"start":{"date-parts":[[2014,5,13]],"date-time":"2014-05-13T00:00:00Z","timestamp":1399939200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[2015,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Sparganosis is a serious food-borne parasitic zoonosis caused by infection with<jats:italic>Spirometra<\/jats:italic>spargana. The prevalence of sparganum infection in wild frogs (<jats:italic>Rana nigromaculata<\/jats:italic>,<jats:italic>R. limmochari<\/jats:italic>,<jats:italic>R. temporaria<\/jats:italic>and<jats:italic>Bufo gargarizans<\/jats:italic>) was investigated in Henan Province of central China during 2008\u20132012. Of 3482 caught wild frogs, 565 (16.23%) were found to be infected with plerocercoids (spargana) of the genus<jats:italic>Spirometra<\/jats:italic>. Spargana were found in 14.85% (320\/2155) of<jats:italic>R. nigromaculata<\/jats:italic>, 20.82% (233\/1119) of<jats:italic>R. limmochari<\/jats:italic>and 10.91% (12\/110) of<jats:italic>R. temporaria<\/jats:italic>frogs. However, no sparganum was found in<jats:italic>B. gargarizans<\/jats:italic>. To investigate the phylogenetic position of collected spargana, three mitochondrial DNA (mtDNA) regions, namely cytochrome<jats:italic>c<\/jats:italic>oxidase subunits 1 and 3 (<jats:italic>cox<\/jats:italic>1 and<jats:italic>cox<\/jats:italic>3), and NADH dehydrogenase subunit 4 (<jats:italic>nad<\/jats:italic>4), were amplified, sequenced and analysed. Sequences of<jats:italic>cox<\/jats:italic>1,<jats:italic>cox<\/jats:italic>3 and p<jats:italic>nad<\/jats:italic>4 were 417, 390 and 578\u00a0bp in length, respectively. The base composition of<jats:italic>cox<\/jats:italic>1,<jats:italic>cox<\/jats:italic>3 and p<jats:italic>nad<\/jats:italic>4 were generally AT rich with a mean of 63.5%, 68.3% and 67% AT, respectively. Phylogenetic analysis showed that all the sparganum isolates in Henan Province represented<jats:italic>Spirometra erinaceieuropaei<\/jats:italic>and were a well-supported clade. These findings demonstrated clearly the usefulness of the three mtDNA sequences for molecular identification and population genetics studies of<jats:italic>S. erinaceieuropaei<\/jats:italic>spargana of human and animal health significance.<\/jats:p>","DOI":"10.1017\/s0022149x14000248","type":"journal-article","created":{"date-parts":[[2014,5,13]],"date-time":"2014-05-13T10:48:47Z","timestamp":1399978127000},"page":"433-438","source":"Crossref","is-referenced-by-count":16,"title":["Levels of sparganum infections and phylogenetic analysis of the tapeworm<i>Spirometra erinaceieuropaei<\/i>sparganum in wild frogs from Henan Province in central China"],"prefix":"10.1017","volume":"89","author":[{"given":"T.","family":"Wei","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"X.","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Cui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.N.","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Z.Q.","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2014,5,13]]},"reference":[{"key":"S0022149X14000248_ref27","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.0b013e3181c91a15"},{"key":"S0022149X14000248_ref3","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(92)90109-W"},{"key":"S0022149X14000248_ref17","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2009.07.001"},{"key":"S0022149X14000248_ref1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2011.03.011"},{"key":"S0022149X14000248_ref14","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2010.06.005"},{"key":"S0022149X14000248_ref25","volume-title":"Foundations of parasitology","author":"Roberts","year":"2009"},{"key":"S0022149X14000248_ref23","unstructured":"Rambaut A. 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