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Helminthol."],"published-print":{"date-parts":[[2015,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>To shed light on the epidemiology of ascariasis in Ecuador and Zanzibar, 177 adult worms retrieved by chemo-expulsion from either people or pigs were collected, measured and subjected to polymerase chain reaction\u2013restriction fragment length polymorphism (PCR-RFLP) analysis of the ribosomal internal transcribed spacer (ITS) region. Upon double digestion with<jats:italic>Rsa<\/jats:italic>I and<jats:italic>Hae<\/jats:italic>III, PCR-RFLP analysis revealed the presence of<jats:italic>A. lumbricoides<\/jats:italic>in people and<jats:italic>A. suum<\/jats:italic>in pigs in Ecuador. In contrast, while there are no pigs on Zanzibar, of the 56 worms obtained from people, one was genotyped as<jats:italic>A. suum<\/jats:italic>. No additional genetic variation was detected upon further PCR-RFLP analysis with several other restriction enzymes. Upon measurement, worm mass and length differed by location and by species,<jats:italic>A. suum<\/jats:italic>being lighter and longer. While there is no evidence to suggest zoonotic transmission in Ecuador, an enduring historical signature of previous zoonotic transmission remains on Zanzibar.<\/jats:p>","DOI":"10.1017\/s0022149x14000431","type":"journal-article","created":{"date-parts":[[2014,6,16]],"date-time":"2014-06-16T18:16:09Z","timestamp":1402942569000},"page":"512-515","source":"Crossref","is-referenced-by-count":11,"title":["Characterization of<i>Ascaris<\/i>from Ecuador and Zanzibar"],"prefix":"10.1017","volume":"89","author":[{"given":"A.M.","family":"Sparks","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Betson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Oviedo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Sandoval","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.J.","family":"Cooper","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.R.","family":"Stothard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2014,6,16]]},"reference":[{"key":"S0022149X14000431_ref22","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2012.04.016"},{"key":"S0022149X14000431_ref18","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0000293"},{"key":"S0022149X14000431_ref16","doi-asserted-by":"publisher","DOI":"10.1186\/1756-3305-5-42"},{"key":"S0022149X14000431_ref15","doi-asserted-by":"publisher","DOI":"10.1016\/j.trstmh.2012.06.009"},{"key":"S0022149X14000431_ref14","doi-asserted-by":"publisher","DOI":"10.2307\/3276967"},{"key":"S0022149X14000431_ref13","doi-asserted-by":"crossref","first-page":"706","DOI":"10.1002\/9781118625590","volume-title":"Applied regression analysis","author":"Draper","year":"1998"},{"key":"S0022149X14000431_ref11","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200211-1320OC"},{"key":"S0022149X14000431_ref10","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0002170"},{"key":"S0022149X14000431_ref9","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-396978-1.00013-6"},{"key":"S0022149X14000431_ref8","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiu193"},{"key":"S0022149X14000431_ref7","first-page":"266","volume-title":"Ascaris: the neglected parasite","author":"Betson","year":"2013"},{"key":"S0022149X14000431_ref6","doi-asserted-by":"publisher","DOI":"10.1016\/j.trstmh.2011.04.009"},{"key":"S0022149X14000431_ref3","doi-asserted-by":"crossref","first-page":"447","DOI":"10.7883\/yoken.63.447","article-title":"Ascariasis in Japan: is pig-derived Ascaris infecting humans?","volume":"63","author":"Arizono","year":"2010","journal-title":"Japanese Journal of Infectious Diseases"},{"key":"S0022149X14000431_ref2","doi-asserted-by":"publisher","DOI":"10.1016\/S1471-4922(00)01944-9"},{"key":"S0022149X14000431_ref12","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3156.1997.d01-320.x"},{"key":"S0022149X14000431_ref20","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182008004836"},{"key":"S0022149X14000431_ref1","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000063988"},{"key":"S0022149X14000431_ref5","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(06)68653-4"},{"key":"S0022149X14000431_ref19","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.3.1142-1148.2005"},{"key":"S0022149X14000431_ref21","first-page":"151","article-title":"Experimental infection of man with Ascaris of man and the pig","volume":"23","author":"Takata","year":"1951","journal-title":"Kitasato Archives of Experimental Medicine"},{"key":"S0022149X14000431_ref4","doi-asserted-by":"publisher","DOI":"10.3201\/eid1710.101826"},{"key":"S0022149X14000431_ref17","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2011.10.043"}],"container-title":["Journal of Helminthology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0022149X14000431","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,8]],"date-time":"2022-04-08T12:03:17Z","timestamp":1649419397000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0022149X14000431\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,6,16]]},"references-count":22,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2015,7]]}},"alternative-id":["S0022149X14000431"],"URL":"https:\/\/doi.org\/10.1017\/s0022149x14000431","relation":{},"ISSN":["0022-149X","1475-2697"],"issn-type":[{"value":"0022-149X","type":"print"},{"value":"1475-2697","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,6,16]]}}}