{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T03:11:19Z","timestamp":1767928279229,"version":"3.49.0"},"reference-count":38,"publisher":"Cambridge University Press (CUP)","issue":"1","license":[{"start":{"date-parts":[[2014,10,2]],"date-time":"2014-10-02T00:00:00Z","timestamp":1412208000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[2016,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Leucine aminopeptidase (LAP) and cathepsin L1 (CL1) are important enzymes for the pathogenesis and physiology of<jats:italic>Fasciola hepatica<\/jats:italic>. These enzymes were analysed<jats:italic>in silico<\/jats:italic>to design a chimeric protein containing the most antigenic sequences of LAP (GenBank; AAV59016.1; amino acids 192\u2013281) and CL1 (GenBank CAC12806.1; amino acids 173\u2013309). The cloned 681-bp chimeric fragment (rFhLAP-CL1) contains 270\u00a0bp from LAP and 411\u00a0bp from CL1, comprising three epitopes, DGRVVHLKY (amino acids 54\u201362) from LAP, VTGYYTVHSGSEVELKNLV (amino acids 119\u2013137) and YQSQTCLPF (amino acids 161\u2013169) from CL1. The ~25\u00a0kDa rFhLAP-CL1 chimeric protein was expressed from the pET15b plasmid in the Rosetta (DE3)<jats:italic>Escherichia coli<\/jats:italic>strain. The chimeric protein rFhLAP-CL1, which showed antigenic and immunogenic properties, was recognized in Western blot assays using<jats:italic>F. hepatica<\/jats:italic>-positive bovine sera, and induced strong, specific antibody responses following immunization in rabbits. The newly generated chimeric protein may be used as a diagnostic tool for detection of antibodies against<jats:italic>F. hepatica<\/jats:italic>in bovine sera and as an immunogen to induce protection against bovine fasciolosis.<\/jats:p>","DOI":"10.1017\/s0022149x14000686","type":"journal-article","created":{"date-parts":[[2014,10,2]],"date-time":"2014-10-02T07:09:36Z","timestamp":1412233776000},"page":"7-13","source":"Crossref","is-referenced-by-count":9,"title":["Construction and evaluation of a chimeric protein made from<i>Fasciola hepatica<\/i>leucine aminopeptidase and cathepsin L1"],"prefix":"10.1017","volume":"90","author":[{"given":"K.","family":"Hern\u00e1ndez-Guzm\u00e1n","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Sahag\u00fan-Ruiz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.J.","family":"Vallecillo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I.","family":"Cruz-Mendoza","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Quiroz-Romero","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2014,10,2]]},"reference":[{"key":"S0022149X14000686_ref29","doi-asserted-by":"crossref","unstructured":"Saha S. & Raghava G.P.S. (2004) BcePred: Prediction of continuous B-cell epitopes in antigenic sequences using physico-chemical properties. pp. 197\u2013204 in Nicosia G. , Cutello V. , Bentley P.J. & Timis J. (Eds) Third International Conference on Artificial Immune Systems, Catania, Italy, 13\u201316 September. Berlin, Springer.","DOI":"10.1007\/978-3-540-30220-9_16"},{"key":"S0022149X14000686_ref38","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcpa.2010.01.011"},{"key":"S0022149X14000686_ref37","doi-asserted-by":"publisher","DOI":"10.1023\/A:1008848517221"},{"key":"S0022149X14000686_ref35","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.9.4350"},{"key":"S0022149X14000686_ref34","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-308X(08)60243-2"},{"key":"S0022149X14000686_ref33","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/22.22.4673"},{"key":"S0022149X14000686_ref32","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(93)90171-S"},{"key":"S0022149X14000686_ref31","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-6851(01)00438-8"},{"key":"S0022149X14000686_ref19","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"S0022149X14000686_ref24","doi-asserted-by":"publisher","DOI":"10.1053\/rvsc.1998.0270"},{"key":"S0022149X14000686_ref16","doi-asserted-by":"publisher","DOI":"10.1007\/s00239-002-2434-x"},{"key":"S0022149X14000686_ref4","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexmp.2007.01.009"},{"key":"S0022149X14000686_ref36","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2008.01.005"},{"key":"S0022149X14000686_ref22","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2010.01.055"},{"key":"S0022149X14000686_ref26","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2013.01.001"},{"key":"S0022149X14000686_ref2","doi-asserted-by":"publisher","DOI":"10.1136\/vr.159.13.424"},{"key":"S0022149X14000686_ref14","doi-asserted-by":"publisher","DOI":"10.1093\/protein\/10.9.1099"},{"key":"S0022149X14000686_ref7","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(93)90172-T"},{"key":"S0022149X14000686_ref10","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-7519(03)00171-1"},{"key":"S0022149X14000686_ref21","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2011.09.020"},{"key":"S0022149X14000686_ref28","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M700560-MCP200"},{"key":"S0022149X14000686_ref6","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-003-1059-3"},{"key":"S0022149X14000686_ref27","doi-asserted-by":"crossref","first-page":"1954","DOI":"10.1128\/IAI.67.4.1954-1961.1999","article-title":"Vaccination with cathepsin L proteinases with leucine aminopeptidase induces high levels of protection against fascioliasis in sheep","volume":"67","author":"Piacenza","year":"1999","journal-title":"Infection and Immunity"},{"key":"S0022149X14000686_ref1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2007.11.011"},{"key":"S0022149X14000686_ref3","doi-asserted-by":"publisher","DOI":"10.2307\/3284308"},{"key":"S0022149X14000686_ref20","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2008.01.023"},{"key":"S0022149X14000686_ref5","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpddr.2013.11.005"},{"key":"S0022149X14000686_ref18","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(90)80535-Q"},{"key":"S0022149X14000686_ref23","first-page":"399","article-title":"Triclabendazole-resistant liver fluke in Scottish sheep","volume":"143","author":"Mitchell","year":"1998","journal-title":"Veterinary Record"},{"key":"S0022149X14000686_ref15","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00452-12"},{"key":"S0022149X14000686_ref11","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkp985"},{"key":"S0022149X14000686_ref9","doi-asserted-by":"crossref","first-page":"5066","DOI":"10.1128\/IAI.64.12.5066-5074.1996","article-title":"Induction of protective immunity in cattle against infection with Fasciola hepatica by vaccination with cathepsin L proteinases and with hemoglobin","volume":"64","author":"Dalton","year":"1996","journal-title":"Infection and Immunity"},{"key":"S0022149X14000686_ref17","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2008.10.099"},{"key":"S0022149X14000686_ref8","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-7519(01)00175-8"},{"key":"S0022149X14000686_ref30","doi-asserted-by":"publisher","DOI":"10.1002\/prot.21078"},{"key":"S0022149X14000686_ref13","first-page":"167","article-title":"Fasciolosis bovina. Evaluaci\u00f3n de las principales p\u00e9rdidas provocadas en una empresa ganadera","volume":"29","author":"Gonz\u00e1lez","year":"2007","journal-title":"Revista de Salud Animal"},{"key":"S0022149X14000686_ref12","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2010.06.039"},{"key":"S0022149X14000686_ref25","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1998.58.417"}],"container-title":["Journal of Helminthology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0022149X14000686","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,8,25]],"date-time":"2020-08-25T08:57:04Z","timestamp":1598345824000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0022149X14000686\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,10,2]]},"references-count":38,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2016,1]]}},"alternative-id":["S0022149X14000686"],"URL":"https:\/\/doi.org\/10.1017\/s0022149x14000686","relation":{},"ISSN":["0022-149X","1475-2697"],"issn-type":[{"value":"0022-149X","type":"print"},{"value":"1475-2697","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,10,2]]}}}