{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,9,8]],"date-time":"2022-09-08T09:11:54Z","timestamp":1662628314161},"reference-count":49,"publisher":"Cambridge University Press (CUP)","issue":"3","license":[{"start":{"date-parts":[[2015,3,12]],"date-time":"2015-03-12T00:00:00Z","timestamp":1426118400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[2016,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Hymenolepis diminuta<\/jats:italic> is a natural parasite of the common brown rat <jats:italic>Rattus norvegicus<\/jats:italic>, and provides a convenient model system for the assessment of the anthelmintic activity of novel drugs against cestodes. The experiments described in this paper indicate that treatment of rats infected with <jats:italic>H. diminuta<\/jats:italic> with a supernatant extract of papaya latex, containing a mixture of four cysteine proteinases, was moderately efficacious, resulting in a significant, but relatively small, reduction in worm burden and biomass. However, faecal egg output was not affected by treatment. In our experiments these effects were only partially dose-dependent, although specific inhibition by E-64 confirmed the role of cysteine proteinases as the active principles in papaya latex affecting worm growth but not statistically reducing worm burden. Data collected for a further 7 days after treatment indicated that the effects of papaya latex supernatant on worm loss and on worm growth were not enhanced. Our findings provide a starting point for further refinement in formulation and delivery, or assessment of alternative natural plant-derived cysteine proteinases in efforts to develop these naturally occurring enzymes into broad-spectrum anthelmintics, with efficacy against cestodes as well as nematodes.<\/jats:p>","DOI":"10.1017\/s0022149x15000127","type":"journal-article","created":{"date-parts":[[2015,3,12]],"date-time":"2015-03-12T06:43:42Z","timestamp":1426142622000},"page":"284-293","source":"Crossref","is-referenced-by-count":4,"title":["The anthelmintic efficacy of natural plant cysteine proteinases against the rat tapeworm <i>Hymenolepis diminuta<\/i><i>in 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